Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3283 and Pf1N1B4_3284 are separated by 56 nucleotides Pf1N1B4_3284 and Pf1N1B4_3285 are separated by 36 nucleotides Pf1N1B4_3285 and Pf1N1B4_3286 are separated by 265 nucleotides
Pf1N1B4_3283 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 3,670,248 to 3,671,018
_3283
Pf1N1B4_3284 - Antitoxin 1, at 3,671,075 to 3,671,482
_3284
Pf1N1B4_3285 - FIG00954883: hypothetical protein, at 3,671,519 to 3,671,698
_3285
Pf1N1B4_3286 - hypothetical protein, at 3,671,964 to 3,672,299
_3286
Position (kb)
3671
3672 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3670.101 kb on + strand at 3670.101 kb on + strand at 3670.101 kb on + strand at 3670.102 kb on - strand at 3670.102 kb on - strand at 3670.102 kb on - strand at 3670.102 kb on - strand at 3670.102 kb on - strand at 3670.189 kb on + strand at 3670.189 kb on + strand at 3670.190 kb on - strand at 3670.247 kb on + strand at 3670.269 kb on + strand at 3670.269 kb on + strand at 3670.269 kb on + strand at 3670.270 kb on - strand at 3670.274 kb on - strand at 3670.274 kb on - strand at 3670.274 kb on - strand at 3670.373 kb on + strand, within Pf1N1B4_3283 at 3670.374 kb on - strand, within Pf1N1B4_3283 at 3670.755 kb on + strand, within Pf1N1B4_3283 at 3670.755 kb on + strand, within Pf1N1B4_3283 at 3670.755 kb on + strand, within Pf1N1B4_3283 at 3670.756 kb on - strand, within Pf1N1B4_3283 at 3670.758 kb on + strand, within Pf1N1B4_3283 at 3670.758 kb on + strand, within Pf1N1B4_3283 at 3670.758 kb on + strand, within Pf1N1B4_3283 at 3670.759 kb on - strand, within Pf1N1B4_3283 at 3670.759 kb on - strand, within Pf1N1B4_3283 at 3670.795 kb on + strand, within Pf1N1B4_3283 at 3670.795 kb on + strand, within Pf1N1B4_3283 at 3670.795 kb on + strand, within Pf1N1B4_3283 at 3670.795 kb on + strand, within Pf1N1B4_3283 at 3670.795 kb on + strand, within Pf1N1B4_3283 at 3670.796 kb on - strand, within Pf1N1B4_3283 at 3670.796 kb on - strand, within Pf1N1B4_3283 at 3670.796 kb on - strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.809 kb on + strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.810 kb on - strand, within Pf1N1B4_3283 at 3670.812 kb on + strand, within Pf1N1B4_3283 at 3670.812 kb on + strand, within Pf1N1B4_3283 at 3670.812 kb on + strand, within Pf1N1B4_3283 at 3670.812 kb on + strand, within Pf1N1B4_3283 at 3670.813 kb on - strand, within Pf1N1B4_3283 at 3670.813 kb on - strand, within Pf1N1B4_3283 at 3670.813 kb on - strand, within Pf1N1B4_3283 at 3670.870 kb on + strand, within Pf1N1B4_3283 at 3670.914 kb on + strand, within Pf1N1B4_3283 at 3670.914 kb on + strand, within Pf1N1B4_3283 at 3670.914 kb on + strand, within Pf1N1B4_3283 at 3670.914 kb on + strand, within Pf1N1B4_3283 at 3670.915 kb on - strand, within Pf1N1B4_3283 at 3670.926 kb on + strand, within Pf1N1B4_3283 at 3670.926 kb on + strand, within Pf1N1B4_3283 at 3671.021 kb on + strand at 3671.021 kb on + strand at 3671.021 kb on + strand at 3671.021 kb on + strand at 3671.021 kb on + strand at 3671.021 kb on + strand at 3671.022 kb on - strand at 3671.022 kb on - strand at 3671.022 kb on - strand at 3671.150 kb on + strand, within Pf1N1B4_3284 at 3671.150 kb on + strand at 3671.151 kb on - strand, within Pf1N1B4_3284 at 3671.151 kb on - strand, within Pf1N1B4_3284 at 3671.151 kb on - strand at 3671.151 kb on - strand, within Pf1N1B4_3284 at 3671.151 kb on - strand at 3671.151 kb on - strand, within Pf1N1B4_3284 at 3671.151 kb on - strand, within Pf1N1B4_3284 at 3671.151 kb on - strand, within Pf1N1B4_3284 at 3671.154 kb on + strand, within Pf1N1B4_3284 at 3671.154 kb on + strand, within Pf1N1B4_3284 at 3671.154 kb on + strand, within Pf1N1B4_3284 at 3671.155 kb on - strand, within Pf1N1B4_3284 at 3671.155 kb on - strand, within Pf1N1B4_3284 at 3671.179 kb on - strand, within Pf1N1B4_3284 at 3671.220 kb on + strand, within Pf1N1B4_3284 at 3671.221 kb on - strand at 3671.221 kb on - strand, within Pf1N1B4_3284 at 3671.522 kb on + strand at 3671.522 kb on + strand at 3671.522 kb on + strand at 3671.522 kb on + strand at 3671.522 kb on + strand at 3671.523 kb on - strand at 3671.523 kb on - strand at 3671.523 kb on - strand at 3671.523 kb on - strand at 3671.523 kb on - strand at 3671.542 kb on + strand, within Pf1N1B4_3285 at 3671.543 kb on - strand, within Pf1N1B4_3285 at 3671.653 kb on - strand, within Pf1N1B4_3285 at 3671.727 kb on - strand at 3671.729 kb on + strand at 3671.729 kb on + strand at 3671.729 kb on + strand at 3671.729 kb on + strand at 3671.730 kb on - strand at 3671.732 kb on - strand at 3671.732 kb on - strand at 3671.811 kb on + strand at 3671.811 kb on + strand at 3671.812 kb on - strand at 3671.812 kb on - strand at 3671.855 kb on - strand at 3671.908 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.959 kb on - strand at 3671.965 kb on + strand at 3671.966 kb on - strand at 3671.966 kb on - strand at 3671.966 kb on - strand at 3671.997 kb on - strand at 3671.997 kb on - strand at 3671.998 kb on + strand, within Pf1N1B4_3286 at 3671.998 kb on + strand, within Pf1N1B4_3286 at 3671.998 kb on + strand, within Pf1N1B4_3286 at 3671.998 kb on + strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3671.999 kb on - strand, within Pf1N1B4_3286 at 3672.065 kb on - strand, within Pf1N1B4_3286 at 3672.065 kb on - strand, within Pf1N1B4_3286 at 3672.065 kb on - strand, within Pf1N1B4_3286 at 3672.065 kb on - strand, within Pf1N1B4_3286 at 3672.098 kb on - strand, within Pf1N1B4_3286 at 3672.334 kb on - strand at 3672.334 kb on - strand at 3672.334 kb on - strand at 3672.334 kb on - strand at 3672.334 kb on - strand at 3672.334 kb on - strand at 3672.334 kb on - strand at 3672.399 kb on - strand at 3672.399 kb on - strand at 3672.400 kb on + strand at 3672.401 kb on - strand at 3672.455 kb on - strand at 3672.469 kb on + strand at 3672.469 kb on + strand at 3672.470 kb on - strand at 3672.470 kb on - strand at 3672.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 3,670,101 + -1.7 3,670,101 + -0.0 3,670,101 + -0.0 3,670,102 - -0.0 3,670,102 - -1.6 3,670,102 - -0.0 3,670,102 - -0.0 3,670,102 - -1.5 3,670,189 + +0.0 3,670,189 + +0.0 3,670,190 - +0.0 3,670,247 + -3.8 3,670,269 + +0.0 3,670,269 + -1.9 3,670,269 + +0.0 3,670,270 - +0.0 3,670,274 - +0.0 3,670,274 - +0.0 3,670,274 - +0.0 3,670,373 + Pf1N1B4_3283 0.16 +0.0 3,670,374 - Pf1N1B4_3283 0.16 +0.0 3,670,755 + Pf1N1B4_3283 0.66 +0.0 3,670,755 + Pf1N1B4_3283 0.66 +0.0 3,670,755 + Pf1N1B4_3283 0.66 +0.0 3,670,756 - Pf1N1B4_3283 0.66 -2.9 3,670,758 + Pf1N1B4_3283 0.66 -1.2 3,670,758 + Pf1N1B4_3283 0.66 -1.7 3,670,758 + Pf1N1B4_3283 0.66 -2.7 3,670,759 - Pf1N1B4_3283 0.66 -1.9 3,670,759 - Pf1N1B4_3283 0.66 -2.8 3,670,795 + Pf1N1B4_3283 0.71 +0.0 3,670,795 + Pf1N1B4_3283 0.71 -3.1 3,670,795 + Pf1N1B4_3283 0.71 -2.0 3,670,795 + Pf1N1B4_3283 0.71 +0.0 3,670,795 + Pf1N1B4_3283 0.71 +0.0 3,670,796 - Pf1N1B4_3283 0.71 -3.1 3,670,796 - Pf1N1B4_3283 0.71 +0.0 3,670,796 - Pf1N1B4_3283 0.71 +0.0 3,670,809 + Pf1N1B4_3283 0.73 -2.7 3,670,809 + Pf1N1B4_3283 0.73 -0.5 3,670,809 + Pf1N1B4_3283 0.73 -1.0 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 -0.3 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 -0.8 3,670,809 + Pf1N1B4_3283 0.73 -1.9 3,670,809 + Pf1N1B4_3283 0.73 -1.4 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 +0.0 3,670,809 + Pf1N1B4_3283 0.73 -1.5 3,670,810 - Pf1N1B4_3283 0.73 +0.0 3,670,810 - Pf1N1B4_3283 0.73 +0.0 3,670,810 - Pf1N1B4_3283 0.73 +0.0 3,670,810 - Pf1N1B4_3283 0.73 +0.0 3,670,810 - Pf1N1B4_3283 0.73 -1.8 3,670,810 - Pf1N1B4_3283 0.73 +0.0 3,670,810 - Pf1N1B4_3283 0.73 -2.9 3,670,810 - Pf1N1B4_3283 0.73 +0.0 3,670,812 + Pf1N1B4_3283 0.73 -2.2 3,670,812 + Pf1N1B4_3283 0.73 +0.0 3,670,812 + Pf1N1B4_3283 0.73 +0.0 3,670,812 + Pf1N1B4_3283 0.73 -0.7 3,670,813 - Pf1N1B4_3283 0.73 +0.0 3,670,813 - Pf1N1B4_3283 0.73 +0.0 3,670,813 - Pf1N1B4_3283 0.73 -3.5 3,670,870 + Pf1N1B4_3283 0.81 +0.0 3,670,914 + Pf1N1B4_3283 0.86 +0.0 3,670,914 + Pf1N1B4_3283 0.86 -2.4 3,670,914 + Pf1N1B4_3283 0.86 -2.4 3,670,914 + Pf1N1B4_3283 0.86 +0.0 3,670,915 - Pf1N1B4_3283 0.87 +0.0 3,670,926 + Pf1N1B4_3283 0.88 +0.0 3,670,926 + Pf1N1B4_3283 0.88 +0.0 3,671,021 + +0.0 3,671,021 + +0.0 3,671,021 + +0.0 3,671,021 + +0.0 3,671,021 + +0.0 3,671,021 + -2.0 3,671,022 - +0.0 3,671,022 - +0.0 3,671,022 - -4.1 3,671,150 + Pf1N1B4_3284 0.18 +0.0 3,671,150 + +0.0 3,671,151 - Pf1N1B4_3284 0.19 +0.0 3,671,151 - Pf1N1B4_3284 0.19 -1.9 3,671,151 - -3.6 3,671,151 - Pf1N1B4_3284 0.19 +0.0 3,671,151 - -0.7 3,671,151 - Pf1N1B4_3284 0.19 +0.0 3,671,151 - Pf1N1B4_3284 0.19 -1.6 3,671,151 - Pf1N1B4_3284 0.19 -0.5 3,671,154 + Pf1N1B4_3284 0.19 +0.0 3,671,154 + Pf1N1B4_3284 0.19 +1.0 3,671,154 + Pf1N1B4_3284 0.19 -2.6 3,671,155 - Pf1N1B4_3284 0.20 -2.5 3,671,155 - Pf1N1B4_3284 0.20 -5.3 3,671,179 - Pf1N1B4_3284 0.25 -2.1 3,671,220 + Pf1N1B4_3284 0.36 +0.0 3,671,221 - +0.0 3,671,221 - Pf1N1B4_3284 0.36 +0.0 3,671,522 + -1.7 3,671,522 + +0.0 3,671,522 + +0.0 3,671,522 + -2.9 3,671,522 + +0.0 3,671,523 - +0.0 3,671,523 - -2.9 3,671,523 - -1.5 3,671,523 - -2.3 3,671,523 - +0.0 3,671,542 + Pf1N1B4_3285 0.13 -2.0 3,671,543 - Pf1N1B4_3285 0.13 -2.7 3,671,653 - Pf1N1B4_3285 0.74 -3.1 3,671,727 - -2.2 3,671,729 + +0.0 3,671,729 + +0.0 3,671,729 + -2.2 3,671,729 + +0.0 3,671,730 - -2.4 3,671,732 - -3.5 3,671,732 - -3.5 3,671,811 + +0.0 3,671,811 + -3.9 3,671,812 - -2.0 3,671,812 - +0.0 3,671,855 - +0.0 3,671,908 - +0.0 3,671,959 - +0.0 3,671,959 - +0.0 3,671,959 - -2.9 3,671,959 - -1.1 3,671,959 - +0.0 3,671,959 - +0.0 3,671,959 - -1.4 3,671,959 - +0.0 3,671,959 - -2.2 3,671,965 + -1.8 3,671,966 - +0.0 3,671,966 - -1.6 3,671,966 - +0.0 3,671,997 - +0.0 3,671,997 - +0.0 3,671,998 + Pf1N1B4_3286 0.10 +0.0 3,671,998 + Pf1N1B4_3286 0.10 +0.0 3,671,998 + Pf1N1B4_3286 0.10 -2.0 3,671,998 + Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 -0.9 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - -2.9 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,671,999 - Pf1N1B4_3286 0.10 +0.0 3,672,065 - Pf1N1B4_3286 0.30 +0.0 3,672,065 - Pf1N1B4_3286 0.30 +0.0 3,672,065 - Pf1N1B4_3286 0.30 +0.0 3,672,065 - Pf1N1B4_3286 0.30 +0.0 3,672,098 - Pf1N1B4_3286 0.40 -2.7 3,672,334 - +0.0 3,672,334 - +0.0 3,672,334 - +0.0 3,672,334 - +0.0 3,672,334 - -2.1 3,672,334 - +0.0 3,672,334 - +0.0 3,672,399 - +0.0 3,672,399 - +0.0 3,672,400 + -1.6 3,672,401 - +0.0 3,672,455 - +0.0 3,672,469 + -3.2 3,672,469 + +0.0 3,672,470 - +0.0 3,672,470 - +0.0 3,672,470 - +0.0
Or see this region's nucleotide sequence