Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3077 and Pf1N1B4_3078 are separated by 33 nucleotides Pf1N1B4_3078 and Pf1N1B4_3079 are separated by 265 nucleotides
Pf1N1B4_3077 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,459,950 to 3,460,984
_3077
Pf1N1B4_3078 - FIG138056: a glutathione-dependent thiol reductase, at 3,461,018 to 3,461,380
_3078
Pf1N1B4_3079 - Transcription-repair coupling factor (superfamily II helicase), at 3,461,646 to 3,462,377
_3079
Position (kb)
3461
3462 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3460.997 kb on - strand at 3461.020 kb on - strand at 3461.020 kb on - strand at 3461.020 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.284 kb on - strand, within Pf1N1B4_3078 at 3461.284 kb on - strand, within Pf1N1B4_3078 at 3461.410 kb on + strand at 3461.411 kb on - strand at 3461.411 kb on - strand at 3461.411 kb on - strand at 3461.425 kb on + strand at 3461.426 kb on - strand at 3461.426 kb on - strand at 3461.473 kb on + strand at 3461.473 kb on + strand at 3461.559 kb on - strand at 3461.559 kb on - strand at 3461.559 kb on - strand at 3461.591 kb on + strand at 3461.591 kb on + strand at 3461.591 kb on + strand at 3461.592 kb on - strand at 3461.592 kb on - strand at 3461.592 kb on - strand at 3461.691 kb on - strand at 3461.777 kb on - strand, within Pf1N1B4_3079 at 3461.777 kb on - strand, within Pf1N1B4_3079 at 3461.784 kb on - strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.891 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.069 kb on - strand, within Pf1N1B4_3079 at 3462.134 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.137 kb on - strand, within Pf1N1B4_3079 at 3462.200 kb on + strand, within Pf1N1B4_3079 at 3462.312 kb on - strand at 3462.322 kb on + strand at 3462.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 3,460,997 - -0.0 3,461,020 - -0.0 3,461,020 - -0.0 3,461,020 - -2.3 3,461,038 - -0.0 3,461,038 - -1.2 3,461,038 - -0.0 3,461,038 - -0.0 3,461,042 - -1.8 3,461,042 - -0.0 3,461,042 - -2.1 3,461,042 - -2.8 3,461,042 - -0.0 3,461,042 - -0.0 3,461,042 - -0.0 3,461,269 - Pf1N1B4_3078 0.69 -0.0 3,461,269 - Pf1N1B4_3078 0.69 -3.7 3,461,269 - Pf1N1B4_3078 0.69 -0.0 3,461,269 - Pf1N1B4_3078 0.69 -3.9 3,461,284 - Pf1N1B4_3078 0.73 -0.0 3,461,284 - Pf1N1B4_3078 0.73 -0.0 3,461,410 + -3.7 3,461,411 - -0.0 3,461,411 - -2.0 3,461,411 - -0.0 3,461,425 + -0.4 3,461,426 - -1.8 3,461,426 - -1.3 3,461,473 + -0.0 3,461,473 + -3.3 3,461,559 - -2.9 3,461,559 - -2.0 3,461,559 - -1.6 3,461,591 + -1.7 3,461,591 + -0.0 3,461,591 + -0.0 3,461,592 - -2.7 3,461,592 - -0.0 3,461,592 - -0.0 3,461,691 - -1.7 3,461,777 - Pf1N1B4_3079 0.18 -2.6 3,461,777 - Pf1N1B4_3079 0.18 -0.0 3,461,784 - Pf1N1B4_3079 0.19 -3.5 3,461,785 + Pf1N1B4_3079 0.19 -0.0 3,461,785 + Pf1N1B4_3079 0.19 +1.4 3,461,785 + Pf1N1B4_3079 0.19 -0.0 3,461,785 + Pf1N1B4_3079 0.19 -0.0 3,461,786 - Pf1N1B4_3079 0.19 -0.0 3,461,786 - Pf1N1B4_3079 0.19 -0.0 3,461,786 - Pf1N1B4_3079 0.19 -0.0 3,461,879 - Pf1N1B4_3079 0.32 -0.0 3,461,879 - Pf1N1B4_3079 0.32 -0.0 3,461,879 - Pf1N1B4_3079 0.32 -0.0 3,461,891 + Pf1N1B4_3079 0.33 -2.3 3,462,065 + Pf1N1B4_3079 0.57 -0.0 3,462,065 + Pf1N1B4_3079 0.57 -0.0 3,462,065 + Pf1N1B4_3079 0.57 -2.1 3,462,066 - Pf1N1B4_3079 0.57 -0.0 3,462,066 - Pf1N1B4_3079 0.57 -0.0 3,462,066 - Pf1N1B4_3079 0.57 -2.7 3,462,066 - Pf1N1B4_3079 0.57 -2.0 3,462,066 - Pf1N1B4_3079 0.57 -0.0 3,462,066 - Pf1N1B4_3079 0.57 -0.0 3,462,066 - Pf1N1B4_3079 0.57 -1.5 3,462,066 - Pf1N1B4_3079 0.57 -1.6 3,462,069 - Pf1N1B4_3079 0.58 -0.0 3,462,134 + Pf1N1B4_3079 0.67 -2.5 3,462,136 + Pf1N1B4_3079 0.67 -0.0 3,462,136 + Pf1N1B4_3079 0.67 -0.0 3,462,136 + Pf1N1B4_3079 0.67 -2.5 3,462,136 + Pf1N1B4_3079 0.67 -0.0 3,462,136 + Pf1N1B4_3079 0.67 -0.0 3,462,137 - Pf1N1B4_3079 0.67 -2.0 3,462,200 + Pf1N1B4_3079 0.76 -0.0 3,462,312 - -2.4 3,462,322 + -1.9 3,462,378 + -2.3
Or see this region's nucleotide sequence