Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2975

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2973 and Pf1N1B4_2974 overlap by 4 nucleotidesPf1N1B4_2974 and Pf1N1B4_2975 are separated by 154 nucleotidesPf1N1B4_2975 and Pf1N1B4_2976 are separated by 43 nucleotides Pf1N1B4_2973 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,334,257 to 3,334,721 _2973 Pf1N1B4_2974 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 3,334,718 to 3,335,434 _2974 Pf1N1B4_2975 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 3,335,589 to 3,336,104 _2975 Pf1N1B4_2976 - DipZ protein, at 3,336,148 to 3,337,935 _2976 Position (kb) 3335 3336 3337Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3334.750 kb on - strandat 3334.849 kb on - strand, within Pf1N1B4_2974at 3334.929 kb on + strand, within Pf1N1B4_2974at 3334.929 kb on + strand, within Pf1N1B4_2974at 3334.929 kb on + strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3334.930 kb on - strand, within Pf1N1B4_2974at 3335.130 kb on + strand, within Pf1N1B4_2974at 3335.378 kb on - strandat 3335.494 kb on + strandat 3335.494 kb on + strandat 3335.495 kb on - strandat 3335.508 kb on + strandat 3335.544 kb on + strandat 3335.544 kb on + strandat 3335.545 kb on - strandat 3335.545 kb on - strandat 3335.656 kb on + strand, within Pf1N1B4_2975at 3335.657 kb on - strand, within Pf1N1B4_2975at 3335.657 kb on - strand, within Pf1N1B4_2975at 3335.806 kb on - strandat 3335.806 kb on - strand, within Pf1N1B4_2975at 3335.834 kb on + strand, within Pf1N1B4_2975at 3335.835 kb on - strand, within Pf1N1B4_2975at 3336.060 kb on + strandat 3336.102 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.147 kb on + strandat 3336.148 kb on - strandat 3336.148 kb on - strandat 3336.169 kb on + strandat 3336.170 kb on - strandat 3336.356 kb on - strand, within Pf1N1B4_2976at 3336.768 kb on - strand, within Pf1N1B4_2976at 3336.867 kb on + strand, within Pf1N1B4_2976at 3337.081 kb on + strand, within Pf1N1B4_2976at 3337.081 kb on + strand, within Pf1N1B4_2976at 3337.081 kb on + strand, within Pf1N1B4_2976at 3337.081 kb on + strand, within Pf1N1B4_2976at 3337.082 kb on - strand, within Pf1N1B4_2976at 3337.082 kb on - strand, within Pf1N1B4_2976

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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3,334,750 - -1.1
3,334,849 - Pf1N1B4_2974 0.18 -0.0
3,334,929 + Pf1N1B4_2974 0.29 -1.4
3,334,929 + Pf1N1B4_2974 0.29 -0.0
3,334,929 + Pf1N1B4_2974 0.29 +1.0
3,334,930 - Pf1N1B4_2974 0.30 -0.0
3,334,930 - Pf1N1B4_2974 0.30 -1.3
3,334,930 - Pf1N1B4_2974 0.30 -0.0
3,334,930 - Pf1N1B4_2974 0.30 -2.6
3,334,930 - Pf1N1B4_2974 0.30 -2.9
3,335,130 + Pf1N1B4_2974 0.57 -3.7
3,335,378 - -0.0
3,335,494 + -2.8
3,335,494 + -0.0
3,335,495 - -0.0
3,335,508 + -1.8
3,335,544 + -2.4
3,335,544 + -0.0
3,335,545 - -2.4
3,335,545 - -0.0
3,335,656 + Pf1N1B4_2975 0.13 -0.0
3,335,657 - Pf1N1B4_2975 0.13 -3.8
3,335,657 - Pf1N1B4_2975 0.13 -0.0
3,335,806 - -0.0
3,335,806 - Pf1N1B4_2975 0.42 -0.0
3,335,834 + Pf1N1B4_2975 0.47 -0.0
3,335,835 - Pf1N1B4_2975 0.48 -0.0
3,336,060 + -2.0
3,336,102 + -0.0
3,336,147 + -0.0
3,336,147 + -0.0
3,336,147 + -0.0
3,336,147 + -0.0
3,336,147 + -0.0
3,336,147 + -2.2
3,336,148 - -1.7
3,336,148 - -4.2
3,336,169 + -0.0
3,336,170 - -2.7
3,336,356 - Pf1N1B4_2976 0.12 -0.0
3,336,768 - Pf1N1B4_2976 0.35 -0.0
3,336,867 + Pf1N1B4_2976 0.40 -0.0
3,337,081 + Pf1N1B4_2976 0.52 -3.3
3,337,081 + Pf1N1B4_2976 0.52 -0.8
3,337,081 + Pf1N1B4_2976 0.52 -3.7
3,337,081 + Pf1N1B4_2976 0.52 -0.0
3,337,082 - Pf1N1B4_2976 0.52 -0.0
3,337,082 - Pf1N1B4_2976 0.52 -1.0

Or see this region's nucleotide sequence