Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2841

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2839 and Pf1N1B4_2840 are separated by 8 nucleotidesPf1N1B4_2840 and Pf1N1B4_2841 are separated by 1 nucleotidesPf1N1B4_2841 and Pf1N1B4_2842 are separated by 2 nucleotides Pf1N1B4_2839 - Dephospho-CoA kinase (EC 2.7.1.24), at 3,183,777 to 3,184,388 _2839 Pf1N1B4_2840 - Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-), at 3,184,397 to 3,185,269 _2840 Pf1N1B4_2841 - Type IV fimbrial assembly protein PilC, at 3,185,271 to 3,186,488 _2841 Pf1N1B4_2842 - Type IV fimbrial assembly, ATPase PilB, at 3,186,491 to 3,188,191 _2842 Position (kb) 3185 3186 3187Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3184.395 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.423 kb on - strandat 3184.434 kb on - strandat 3184.434 kb on - strandat 3184.480 kb on - strandat 3184.673 kb on + strand, within Pf1N1B4_2840at 3185.001 kb on + strand, within Pf1N1B4_2840at 3185.001 kb on + strand, within Pf1N1B4_2840at 3185.002 kb on - strandat 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.002 kb on - strand, within Pf1N1B4_2840at 3185.073 kb on - strand, within Pf1N1B4_2840at 3185.082 kb on + strand, within Pf1N1B4_2840at 3185.082 kb on + strand, within Pf1N1B4_2840at 3185.082 kb on + strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.083 kb on - strand, within Pf1N1B4_2840at 3185.269 kb on + strandat 3185.269 kb on + strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.270 kb on - strandat 3185.305 kb on + strandat 3185.305 kb on + strandat 3185.306 kb on - strandat 3185.343 kb on + strandat 3185.344 kb on - strandat 3185.346 kb on + strandat 3185.346 kb on + strandat 3185.347 kb on - strandat 3185.347 kb on - strandat 3185.410 kb on + strand, within Pf1N1B4_2841at 3185.410 kb on + strand, within Pf1N1B4_2841at 3185.411 kb on - strand, within Pf1N1B4_2841at 3185.411 kb on - strand, within Pf1N1B4_2841at 3185.496 kb on + strand, within Pf1N1B4_2841at 3185.496 kb on + strand, within Pf1N1B4_2841at 3185.496 kb on + strandat 3185.496 kb on + strand, within Pf1N1B4_2841at 3185.497 kb on - strand, within Pf1N1B4_2841at 3185.497 kb on - strand, within Pf1N1B4_2841at 3185.497 kb on - strand, within Pf1N1B4_2841at 3185.497 kb on - strand, within Pf1N1B4_2841at 3185.497 kb on - strand, within Pf1N1B4_2841at 3185.497 kb on - strand, within Pf1N1B4_2841at 3185.498 kb on + strand, within Pf1N1B4_2841at 3185.498 kb on + strand, within Pf1N1B4_2841at 3185.553 kb on + strand, within Pf1N1B4_2841at 3185.553 kb on + strand, within Pf1N1B4_2841at 3185.559 kb on + strand, within Pf1N1B4_2841at 3185.559 kb on + strand, within Pf1N1B4_2841at 3185.559 kb on + strand, within Pf1N1B4_2841at 3185.559 kb on + strandat 3185.562 kb on + strand, within Pf1N1B4_2841at 3185.562 kb on + strand, within Pf1N1B4_2841at 3185.563 kb on - strand, within Pf1N1B4_2841at 3185.686 kb on + strand, within Pf1N1B4_2841at 3185.686 kb on + strand, within Pf1N1B4_2841at 3185.687 kb on - strand, within Pf1N1B4_2841at 3185.687 kb on - strandat 3185.687 kb on - strand, within Pf1N1B4_2841at 3185.971 kb on + strand, within Pf1N1B4_2841at 3185.971 kb on + strand, within Pf1N1B4_2841at 3185.972 kb on - strand, within Pf1N1B4_2841at 3186.022 kb on + strand, within Pf1N1B4_2841at 3186.022 kb on + strand, within Pf1N1B4_2841at 3186.022 kb on + strand, within Pf1N1B4_2841at 3186.022 kb on + strand, within Pf1N1B4_2841at 3186.023 kb on - strand, within Pf1N1B4_2841at 3186.023 kb on - strand, within Pf1N1B4_2841at 3186.023 kb on - strand, within Pf1N1B4_2841at 3186.023 kb on - strand, within Pf1N1B4_2841at 3186.023 kb on - strand, within Pf1N1B4_2841at 3186.091 kb on + strand, within Pf1N1B4_2841at 3186.091 kb on + strand, within Pf1N1B4_2841at 3186.091 kb on + strand, within Pf1N1B4_2841at 3186.091 kb on + strandat 3186.091 kb on + strand, within Pf1N1B4_2841at 3186.106 kb on + strand, within Pf1N1B4_2841at 3186.106 kb on + strand, within Pf1N1B4_2841at 3186.107 kb on - strand, within Pf1N1B4_2841at 3186.107 kb on - strand, within Pf1N1B4_2841at 3186.166 kb on - strand, within Pf1N1B4_2841at 3186.240 kb on + strand, within Pf1N1B4_2841at 3186.324 kb on - strand, within Pf1N1B4_2841at 3186.372 kb on + strandat 3186.457 kb on + strandat 3186.457 kb on + strandat 3186.457 kb on + strandat 3186.457 kb on + strandat 3186.458 kb on - strandat 3186.458 kb on - strandat 3186.458 kb on - strandat 3186.458 kb on - strandat 3186.458 kb on - strandat 3186.458 kb on - strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.459 kb on + strandat 3186.460 kb on - strandat 3186.460 kb on - strandat 3186.460 kb on - strandat 3186.460 kb on - strandat 3186.460 kb on - strandat 3186.460 kb on - strandat 3186.460 kb on - strandat 3186.562 kb on + strandat 3186.660 kb on - strandat 3186.669 kb on + strand, within Pf1N1B4_2842at 3186.669 kb on + strand, within Pf1N1B4_2842at 3186.669 kb on + strand, within Pf1N1B4_2842at 3186.669 kb on + strand, within Pf1N1B4_2842at 3186.669 kb on + strand, within Pf1N1B4_2842at 3186.670 kb on - strand, within Pf1N1B4_2842at 3186.670 kb on - strand, within Pf1N1B4_2842at 3186.670 kb on - strand, within Pf1N1B4_2842at 3186.690 kb on + strand, within Pf1N1B4_2842at 3186.690 kb on + strand, within Pf1N1B4_2842at 3186.690 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.726 kb on + strand, within Pf1N1B4_2842at 3186.727 kb on - strand, within Pf1N1B4_2842at 3186.727 kb on - strandat 3186.727 kb on - strand, within Pf1N1B4_2842at 3186.727 kb on - strand, within Pf1N1B4_2842at 3186.727 kb on - strand, within Pf1N1B4_2842at 3186.727 kb on - strand, within Pf1N1B4_2842at 3187.144 kb on + strand, within Pf1N1B4_2842at 3187.144 kb on + strand, within Pf1N1B4_2842at 3187.144 kb on + strand, within Pf1N1B4_2842at 3187.144 kb on + strand, within Pf1N1B4_2842at 3187.145 kb on - strand, within Pf1N1B4_2842at 3187.182 kb on + strand, within Pf1N1B4_2842at 3187.183 kb on - strandat 3187.183 kb on - strandat 3187.183 kb on - strand, within Pf1N1B4_2842at 3187.183 kb on - strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strandat 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.260 kb on + strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.261 kb on - strand, within Pf1N1B4_2842at 3187.334 kb on + strand, within Pf1N1B4_2842at 3187.334 kb on + strand, within Pf1N1B4_2842at 3187.334 kb on + strand, within Pf1N1B4_2842at 3187.334 kb on + strandat 3187.334 kb on + strand, within Pf1N1B4_2842at 3187.337 kb on + strand, within Pf1N1B4_2842at 3187.338 kb on - strand, within Pf1N1B4_2842

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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3,184,395 - +0.0
3,184,423 - -0.6
3,184,423 - +0.0
3,184,423 - -1.8
3,184,423 - -2.8
3,184,423 - +0.0
3,184,434 - -1.6
3,184,434 - +0.0
3,184,480 - +0.0
3,184,673 + Pf1N1B4_2840 0.32 +0.0
3,185,001 + Pf1N1B4_2840 0.69 +0.0
3,185,001 + Pf1N1B4_2840 0.69 -4.8
3,185,002 - -4.3
3,185,002 - Pf1N1B4_2840 0.69 +0.0
3,185,002 - Pf1N1B4_2840 0.69 +0.0
3,185,002 - Pf1N1B4_2840 0.69 +0.0
3,185,002 - Pf1N1B4_2840 0.69 -4.5
3,185,073 - Pf1N1B4_2840 0.77 -2.7
3,185,082 + Pf1N1B4_2840 0.78 -3.1
3,185,082 + Pf1N1B4_2840 0.78 -4.9
3,185,082 + Pf1N1B4_2840 0.78 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,083 - Pf1N1B4_2840 0.79 +0.0
3,185,269 + +0.0
3,185,269 + +0.0
3,185,270 - +0.0
3,185,270 - +0.0
3,185,270 - -1.6
3,185,270 - +0.0
3,185,270 - +0.0
3,185,270 - -1.6
3,185,305 + +0.0
3,185,305 + +0.0
3,185,306 - +0.0
3,185,343 + -1.4
3,185,344 - -2.5
3,185,346 + -2.9
3,185,346 + +0.0
3,185,347 - -1.6
3,185,347 - -4.0
3,185,410 + Pf1N1B4_2841 0.11 +0.0
3,185,410 + Pf1N1B4_2841 0.11 +0.0
3,185,411 - Pf1N1B4_2841 0.11 -1.2
3,185,411 - Pf1N1B4_2841 0.11 +0.0
3,185,496 + Pf1N1B4_2841 0.18 +0.0
3,185,496 + Pf1N1B4_2841 0.18 -1.8
3,185,496 + +0.0
3,185,496 + Pf1N1B4_2841 0.18 +0.0
3,185,497 - Pf1N1B4_2841 0.19 +0.0
3,185,497 - Pf1N1B4_2841 0.19 -2.3
3,185,497 - Pf1N1B4_2841 0.19 +0.0
3,185,497 - Pf1N1B4_2841 0.19 +0.0
3,185,497 - Pf1N1B4_2841 0.19 -3.0
3,185,497 - Pf1N1B4_2841 0.19 +0.0
3,185,498 + Pf1N1B4_2841 0.19 -2.6
3,185,498 + Pf1N1B4_2841 0.19 -1.8
3,185,553 + Pf1N1B4_2841 0.23 +0.0
3,185,553 + Pf1N1B4_2841 0.23 +0.0
3,185,559 + Pf1N1B4_2841 0.24 +0.0
3,185,559 + Pf1N1B4_2841 0.24 -2.9
3,185,559 + Pf1N1B4_2841 0.24 +0.0
3,185,559 + +0.0
3,185,562 + Pf1N1B4_2841 0.24 +0.0
3,185,562 + Pf1N1B4_2841 0.24 +0.0
3,185,563 - Pf1N1B4_2841 0.24 -0.3
3,185,686 + Pf1N1B4_2841 0.34 +0.0
3,185,686 + Pf1N1B4_2841 0.34 +0.0
3,185,687 - Pf1N1B4_2841 0.34 +1.0
3,185,687 - +0.0
3,185,687 - Pf1N1B4_2841 0.34 +0.0
3,185,971 + Pf1N1B4_2841 0.57 +0.0
3,185,971 + Pf1N1B4_2841 0.57 +0.0
3,185,972 - Pf1N1B4_2841 0.58 +0.0
3,186,022 + Pf1N1B4_2841 0.62 +0.0
3,186,022 + Pf1N1B4_2841 0.62 -2.2
3,186,022 + Pf1N1B4_2841 0.62 +0.0
3,186,022 + Pf1N1B4_2841 0.62 +0.0
3,186,023 - Pf1N1B4_2841 0.62 +0.0
3,186,023 - Pf1N1B4_2841 0.62 +0.0
3,186,023 - Pf1N1B4_2841 0.62 -3.0
3,186,023 - Pf1N1B4_2841 0.62 +0.0
3,186,023 - Pf1N1B4_2841 0.62 +0.0
3,186,091 + Pf1N1B4_2841 0.67 +0.0
3,186,091 + Pf1N1B4_2841 0.67 -3.9
3,186,091 + Pf1N1B4_2841 0.67 +0.0
3,186,091 + -2.1
3,186,091 + Pf1N1B4_2841 0.67 +0.0
3,186,106 + Pf1N1B4_2841 0.69 -2.7
3,186,106 + Pf1N1B4_2841 0.69 +0.0
3,186,107 - Pf1N1B4_2841 0.69 +0.0
3,186,107 - Pf1N1B4_2841 0.69 +0.0
3,186,166 - Pf1N1B4_2841 0.73 -3.3
3,186,240 + Pf1N1B4_2841 0.80 +0.0
3,186,324 - Pf1N1B4_2841 0.86 +0.0
3,186,372 + -1.1
3,186,457 + -1.9
3,186,457 + +0.0
3,186,457 + -3.2
3,186,457 + -1.9
3,186,458 - +0.0
3,186,458 - +0.0
3,186,458 - +0.0
3,186,458 - -2.3
3,186,458 - +0.0
3,186,458 - +0.0
3,186,459 + +0.0
3,186,459 + +0.0
3,186,459 + -5.0
3,186,459 + +0.0
3,186,459 + +0.0
3,186,459 + +0.0
3,186,459 + +0.0
3,186,459 + -2.5
3,186,459 + +0.0
3,186,459 + -4.2
3,186,460 - +0.0
3,186,460 - -3.2
3,186,460 - +0.0
3,186,460 - -1.9
3,186,460 - -0.5
3,186,460 - -3.3
3,186,460 - +0.0
3,186,562 + -2.9
3,186,660 - -1.6
3,186,669 + Pf1N1B4_2842 0.10 +0.0
3,186,669 + Pf1N1B4_2842 0.10 +0.0
3,186,669 + Pf1N1B4_2842 0.10 +0.0
3,186,669 + Pf1N1B4_2842 0.10 -2.4
3,186,669 + Pf1N1B4_2842 0.10 -1.0
3,186,670 - Pf1N1B4_2842 0.11 +0.0
3,186,670 - Pf1N1B4_2842 0.11 +0.0
3,186,670 - Pf1N1B4_2842 0.11 +0.0
3,186,690 + Pf1N1B4_2842 0.12 +0.0
3,186,690 + Pf1N1B4_2842 0.12 -2.6
3,186,690 + Pf1N1B4_2842 0.12 +0.0
3,186,726 + Pf1N1B4_2842 0.14 +0.0
3,186,726 + Pf1N1B4_2842 0.14 -2.5
3,186,726 + Pf1N1B4_2842 0.14 +0.0
3,186,726 + Pf1N1B4_2842 0.14 -3.7
3,186,726 + Pf1N1B4_2842 0.14 -3.1
3,186,726 + Pf1N1B4_2842 0.14 +0.0
3,186,726 + Pf1N1B4_2842 0.14 +0.0
3,186,726 + Pf1N1B4_2842 0.14 -2.2
3,186,726 + Pf1N1B4_2842 0.14 +0.0
3,186,727 - Pf1N1B4_2842 0.14 +0.0
3,186,727 - +0.0
3,186,727 - Pf1N1B4_2842 0.14 +0.0
3,186,727 - Pf1N1B4_2842 0.14 +0.0
3,186,727 - Pf1N1B4_2842 0.14 +0.0
3,186,727 - Pf1N1B4_2842 0.14 +0.0
3,187,144 + Pf1N1B4_2842 0.38 -1.7
3,187,144 + Pf1N1B4_2842 0.38 -3.4
3,187,144 + Pf1N1B4_2842 0.38 -1.0
3,187,144 + Pf1N1B4_2842 0.38 +0.0
3,187,145 - Pf1N1B4_2842 0.38 -1.1
3,187,182 + Pf1N1B4_2842 0.41 +0.0
3,187,183 - +0.0
3,187,183 - -2.0
3,187,183 - Pf1N1B4_2842 0.41 +0.0
3,187,183 - Pf1N1B4_2842 0.41 +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + Pf1N1B4_2842 0.45 +0.0
3,187,260 + Pf1N1B4_2842 0.45 -1.9
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 -2.3
3,187,261 - Pf1N1B4_2842 0.45 -1.8
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,261 - Pf1N1B4_2842 0.45 +0.0
3,187,334 + Pf1N1B4_2842 0.50 +0.0
3,187,334 + Pf1N1B4_2842 0.50 -1.9
3,187,334 + Pf1N1B4_2842 0.50 +0.0
3,187,334 + -3.5
3,187,334 + Pf1N1B4_2842 0.50 -1.3
3,187,337 + Pf1N1B4_2842 0.50 +0.0
3,187,338 - Pf1N1B4_2842 0.50 -2.2

Or see this region's nucleotide sequence