Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2805 and Pf1N1B4_2806 overlap by 8 nucleotides Pf1N1B4_2806 and Pf1N1B4_2807 overlap by 8 nucleotides Pf1N1B4_2807 and Pf1N1B4_2808 are separated by 192 nucleotides
Pf1N1B4_2805 - Isoleucyl-tRNA synthetase (EC 6.1.1.5), at 3,148,257 to 3,151,088
_2805
Pf1N1B4_2806 - Lipoprotein signal peptidase (EC 3.4.23.36), at 3,151,081 to 3,151,593
_2806
Pf1N1B4_2807 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 3,151,586 to 3,152,038
_2807
Pf1N1B4_2808 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2), at 3,152,231 to 3,153,178
_2808
Position (kb)
3151
3152
3153 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.600 kb on - strand at 3151.684 kb on + strand, within Pf1N1B4_2807 at 3151.684 kb on + strand, within Pf1N1B4_2807 at 3151.684 kb on + strand, within Pf1N1B4_2807 at 3151.685 kb on - strand, within Pf1N1B4_2807 at 3151.685 kb on - strand at 3151.861 kb on + strand, within Pf1N1B4_2807 at 3151.915 kb on + strand, within Pf1N1B4_2807 at 3151.990 kb on + strand, within Pf1N1B4_2807 at 3152.108 kb on + strand at 3152.108 kb on + strand at 3152.149 kb on + strand at 3152.197 kb on + strand at 3152.200 kb on + strand at 3152.200 kb on + strand at 3152.200 kb on + strand at 3152.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 3,151,551 + -0.0 3,151,551 + -0.0 3,151,551 + -0.0 3,151,551 + -2.4 3,151,551 + -0.0 3,151,599 + -0.0 3,151,599 + -0.0 3,151,599 + -0.0 3,151,599 + -4.3 3,151,599 + -4.7 3,151,599 + -0.0 3,151,600 - -0.0 3,151,684 + Pf1N1B4_2807 0.22 -1.0 3,151,684 + Pf1N1B4_2807 0.22 -2.3 3,151,684 + Pf1N1B4_2807 0.22 -5.4 3,151,685 - Pf1N1B4_2807 0.22 -0.0 3,151,685 - -1.8 3,151,861 + Pf1N1B4_2807 0.61 -0.0 3,151,915 + Pf1N1B4_2807 0.73 -3.8 3,151,990 + Pf1N1B4_2807 0.89 -0.0 3,152,108 + -0.0 3,152,108 + -0.0 3,152,149 + -0.0 3,152,197 + -0.6 3,152,200 + -1.1 3,152,200 + -0.0 3,152,200 + -0.0 3,152,200 + -0.0
Or see this region's nucleotide sequence