Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2688 and Pf1N1B4_2689 are separated by 1 nucleotides Pf1N1B4_2689 and Pf1N1B4_2690 are separated by 72 nucleotides Pf1N1B4_2690 and Pf1N1B4_2691 are separated by 9 nucleotides
Pf1N1B4_2688 - Gamma-glutamyl phosphate reductase (EC 1.2.1.41), at 3,025,180 to 3,026,427
_2688
Pf1N1B4_2689 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 3,026,429 to 3,027,118
_2689
Pf1N1B4_2690 - Iojap protein, at 3,027,191 to 3,027,685
_2690
Pf1N1B4_2691 - LSU m3Psi1915 methyltransferase RlmH, at 3,027,695 to 3,028,162
_2691
Position (kb)
3026
3027
3028 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3025.531 kb on + strand, within Pf1N1B4_2688 at 3025.753 kb on + strand, within Pf1N1B4_2688 at 3025.858 kb on + strand, within Pf1N1B4_2688 at 3025.858 kb on + strand, within Pf1N1B4_2688 at 3025.858 kb on + strand, within Pf1N1B4_2688 at 3025.918 kb on + strand, within Pf1N1B4_2688 at 3026.043 kb on + strand at 3026.098 kb on + strand, within Pf1N1B4_2688 at 3026.157 kb on + strand, within Pf1N1B4_2688 at 3026.157 kb on + strand, within Pf1N1B4_2688 at 3026.157 kb on + strand, within Pf1N1B4_2688 at 3026.160 kb on + strand, within Pf1N1B4_2688 at 3026.262 kb on + strand, within Pf1N1B4_2688 at 3026.322 kb on + strand at 3026.380 kb on + strand at 3026.380 kb on + strand at 3026.380 kb on + strand at 3026.380 kb on + strand at 3026.380 kb on + strand at 3027.152 kb on + strand at 3027.761 kb on + strand, within Pf1N1B4_2691
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 3,025,531 + Pf1N1B4_2688 0.28 -0.0 3,025,753 + Pf1N1B4_2688 0.46 -3.4 3,025,858 + Pf1N1B4_2688 0.54 -0.0 3,025,858 + Pf1N1B4_2688 0.54 -3.6 3,025,858 + Pf1N1B4_2688 0.54 -0.0 3,025,918 + Pf1N1B4_2688 0.59 -0.0 3,026,043 + -0.0 3,026,098 + Pf1N1B4_2688 0.74 -1.0 3,026,157 + Pf1N1B4_2688 0.78 -0.0 3,026,157 + Pf1N1B4_2688 0.78 -0.0 3,026,157 + Pf1N1B4_2688 0.78 -0.0 3,026,160 + Pf1N1B4_2688 0.79 -0.0 3,026,262 + Pf1N1B4_2688 0.87 -0.0 3,026,322 + -0.0 3,026,380 + -3.5 3,026,380 + -0.0 3,026,380 + -0.0 3,026,380 + -3.0 3,026,380 + -0.2 3,027,152 + -0.7 3,027,761 + Pf1N1B4_2691 0.14 -2.2
Or see this region's nucleotide sequence