Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2183 and Pf1N1B4_2184 are separated by 461 nucleotides Pf1N1B4_2184 and Pf1N1B4_2185 are separated by 126 nucleotides
Pf1N1B4_2183 - Argininosuccinate lyase (EC 4.3.2.1), at 2,494,623 to 2,496,017
_2183
Pf1N1B4_2184 - Autolysis response regulater LytR, at 2,496,479 to 2,497,225
_2184
Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293
_2185
Position (kb)
2496
2497
2498 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2496.072 kb on + strand at 2496.072 kb on + strand at 2496.073 kb on - strand at 2496.073 kb on - strand at 2496.172 kb on - strand at 2496.172 kb on - strand at 2496.172 kb on - strand at 2496.190 kb on - strand at 2496.209 kb on - strand at 2496.255 kb on - strand at 2496.255 kb on - strand at 2496.256 kb on + strand at 2496.256 kb on + strand at 2496.256 kb on + strand at 2496.256 kb on + strand at 2496.257 kb on - strand at 2496.257 kb on - strand at 2496.257 kb on - strand at 2496.257 kb on - strand at 2496.257 kb on - strand at 2496.257 kb on - strand at 2496.257 kb on - strand at 2496.277 kb on + strand at 2496.277 kb on + strand at 2496.277 kb on + strand at 2496.277 kb on + strand at 2496.277 kb on + strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.278 kb on - strand at 2496.284 kb on + strand at 2496.284 kb on + strand at 2496.392 kb on + strand at 2496.393 kb on - strand at 2496.436 kb on + strand at 2496.478 kb on + strand at 2496.479 kb on - strand at 2496.479 kb on - strand at 2496.479 kb on - strand at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.753 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.805 kb on - strand, within Pf1N1B4_2184 at 2496.805 kb on - strand, within Pf1N1B4_2184 at 2496.816 kb on + strand, within Pf1N1B4_2184 at 2496.957 kb on - strand, within Pf1N1B4_2184 at 2496.957 kb on - strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2497.243 kb on + strand at 2497.243 kb on + strand at 2497.243 kb on + strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.286 kb on + strand at 2497.286 kb on + strand at 2497.286 kb on + strand at 2497.287 kb on - strand at 2497.287 kb on - strand at 2497.316 kb on + strand at 2497.316 kb on + strand at 2497.317 kb on - strand at 2497.319 kb on + strand at 2497.319 kb on + strand at 2497.319 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 2,496,072 + +0.0 2,496,072 + -0.5 2,496,073 - +0.0 2,496,073 - -2.7 2,496,172 - +0.0 2,496,172 - +0.0 2,496,172 - +0.0 2,496,190 - -1.8 2,496,209 - +0.0 2,496,255 - -2.7 2,496,255 - -3.8 2,496,256 + +0.0 2,496,256 + +0.0 2,496,256 + +0.0 2,496,256 + -0.5 2,496,257 - -2.8 2,496,257 - -3.6 2,496,257 - -2.5 2,496,257 - -2.8 2,496,257 - -2.9 2,496,257 - +0.0 2,496,257 - -2.5 2,496,277 + +0.0 2,496,277 + +0.0 2,496,277 + -1.0 2,496,277 + +0.0 2,496,277 + -2.9 2,496,278 - +0.0 2,496,278 - +0.0 2,496,278 - +0.0 2,496,278 - +0.0 2,496,278 - +0.0 2,496,278 - +0.0 2,496,278 - -3.9 2,496,278 - -2.9 2,496,284 + -2.7 2,496,284 + +0.0 2,496,392 + +0.0 2,496,393 - -4.1 2,496,436 + +0.0 2,496,478 + +0.0 2,496,479 - +0.0 2,496,479 - +0.0 2,496,479 - -3.1 2,496,554 + Pf1N1B4_2184 0.10 -3.5 2,496,554 + Pf1N1B4_2184 0.10 -3.8 2,496,554 + Pf1N1B4_2184 0.10 +0.0 2,496,554 + Pf1N1B4_2184 0.10 +0.0 2,496,554 + Pf1N1B4_2184 0.10 -2.3 2,496,555 - Pf1N1B4_2184 0.10 +0.0 2,496,555 - Pf1N1B4_2184 0.10 +0.0 2,496,555 - Pf1N1B4_2184 0.10 -4.9 2,496,555 - Pf1N1B4_2184 0.10 +0.0 2,496,753 + Pf1N1B4_2184 0.37 +0.0 2,496,804 + Pf1N1B4_2184 0.44 +0.0 2,496,804 + Pf1N1B4_2184 0.44 -2.5 2,496,804 + Pf1N1B4_2184 0.44 +0.0 2,496,804 + Pf1N1B4_2184 0.44 +0.0 2,496,805 - Pf1N1B4_2184 0.44 +0.0 2,496,805 - Pf1N1B4_2184 0.44 +0.0 2,496,816 + Pf1N1B4_2184 0.45 +0.0 2,496,957 - Pf1N1B4_2184 0.64 -4.9 2,496,957 - Pf1N1B4_2184 0.64 +0.0 2,496,977 + Pf1N1B4_2184 0.67 +0.0 2,496,977 + Pf1N1B4_2184 0.67 +0.0 2,496,977 + Pf1N1B4_2184 0.67 +0.0 2,496,977 + Pf1N1B4_2184 0.67 -4.4 2,496,978 - Pf1N1B4_2184 0.67 -4.7 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,496,978 - Pf1N1B4_2184 0.67 +0.0 2,497,243 + +0.0 2,497,243 + +0.0 2,497,243 + +0.0 2,497,244 - +0.0 2,497,244 - +0.0 2,497,244 - +0.0 2,497,244 - +0.0 2,497,286 + +0.0 2,497,286 + +0.0 2,497,286 + +0.0 2,497,287 - +0.0 2,497,287 - -1.9 2,497,316 + +0.0 2,497,316 + +0.0 2,497,317 - -1.7 2,497,319 + +0.0 2,497,319 + +0.0 2,497,319 + -1.4 2,497,332 + +0.0 2,497,332 + +0.0 2,497,332 + +0.0 2,497,332 + -2.2 2,497,332 + -2.2 2,497,332 + +0.0
Or see this region's nucleotide sequence