Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1804

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1802 and Pf1N1B4_1803 are separated by 14 nucleotidesPf1N1B4_1803 and Pf1N1B4_1804 are separated by 199 nucleotidesPf1N1B4_1804 and Pf1N1B4_1805 are separated by 693 nucleotides Pf1N1B4_1802 - Membrane bound c-di-GMP receptor LapD, at 2,068,680 to 2,070,626 _1802 Pf1N1B4_1803 - T1SS associated transglutaminase-like cysteine proteinase LapP, at 2,070,641 to 2,071,333 _1803 Pf1N1B4_1804 - Predicted regulator PutR for proline utilization, GntR family, at 2,071,533 to 2,072,225 _1804 Pf1N1B4_1805 - PROBABLE REMNANT OF A TRANSPOSASE GENE PROTEIN, at 2,072,919 to 2,073,695 _1805 Position (kb) 2071 2072 2073Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2070.642 kb on + strandat 2070.642 kb on + strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.643 kb on - strandat 2070.646 kb on + strandat 2070.646 kb on + strandat 2070.647 kb on - strandat 2070.647 kb on - strandat 2070.647 kb on - strandat 2070.647 kb on - strandat 2070.647 kb on - strandat 2070.781 kb on - strand, within Pf1N1B4_1803at 2070.871 kb on - strand, within Pf1N1B4_1803at 2070.871 kb on - strand, within Pf1N1B4_1803at 2070.871 kb on - strand, within Pf1N1B4_1803at 2070.873 kb on + strand, within Pf1N1B4_1803at 2070.873 kb on + strand, within Pf1N1B4_1803at 2070.873 kb on + strand, within Pf1N1B4_1803at 2070.873 kb on + strandat 2070.874 kb on - strand, within Pf1N1B4_1803at 2070.918 kb on + strand, within Pf1N1B4_1803at 2070.919 kb on - strand, within Pf1N1B4_1803at 2070.919 kb on - strand, within Pf1N1B4_1803at 2070.919 kb on - strand, within Pf1N1B4_1803at 2070.969 kb on + strand, within Pf1N1B4_1803at 2070.969 kb on + strand, within Pf1N1B4_1803at 2070.970 kb on - strand, within Pf1N1B4_1803at 2070.984 kb on + strand, within Pf1N1B4_1803at 2070.985 kb on - strand, within Pf1N1B4_1803at 2071.013 kb on + strand, within Pf1N1B4_1803at 2071.200 kb on + strand, within Pf1N1B4_1803at 2071.201 kb on - strand, within Pf1N1B4_1803at 2071.201 kb on - strand, within Pf1N1B4_1803at 2071.201 kb on - strand, within Pf1N1B4_1803at 2071.429 kb on - strandat 2071.436 kb on - strandat 2071.436 kb on - strandat 2071.436 kb on - strandat 2071.441 kb on - strandat 2071.441 kb on - strandat 2071.441 kb on - strandat 2071.508 kb on - strandat 2071.508 kb on - strandat 2071.562 kb on + strandat 2071.563 kb on - strandat 2071.699 kb on + strand, within Pf1N1B4_1804at 2071.754 kb on + strand, within Pf1N1B4_1804at 2071.754 kb on + strand, within Pf1N1B4_1804at 2071.754 kb on + strand, within Pf1N1B4_1804at 2071.755 kb on - strand, within Pf1N1B4_1804at 2071.755 kb on - strand, within Pf1N1B4_1804at 2071.755 kb on - strand, within Pf1N1B4_1804at 2071.818 kb on + strand, within Pf1N1B4_1804at 2071.818 kb on + strand, within Pf1N1B4_1804at 2071.818 kb on + strand, within Pf1N1B4_1804at 2071.818 kb on + strand, within Pf1N1B4_1804at 2071.818 kb on + strand, within Pf1N1B4_1804at 2071.819 kb on - strand, within Pf1N1B4_1804at 2071.819 kb on - strand, within Pf1N1B4_1804at 2071.819 kb on - strand, within Pf1N1B4_1804at 2071.905 kb on + strand, within Pf1N1B4_1804at 2071.905 kb on + strand, within Pf1N1B4_1804at 2071.906 kb on - strand, within Pf1N1B4_1804at 2071.906 kb on - strand, within Pf1N1B4_1804at 2071.906 kb on - strand, within Pf1N1B4_1804at 2071.906 kb on - strand, within Pf1N1B4_1804at 2071.906 kb on - strand, within Pf1N1B4_1804at 2071.906 kb on - strand, within Pf1N1B4_1804at 2071.908 kb on - strand, within Pf1N1B4_1804at 2071.981 kb on - strandat 2071.995 kb on - strand, within Pf1N1B4_1804at 2071.995 kb on - strand, within Pf1N1B4_1804at 2072.199 kb on + strandat 2072.215 kb on + strandat 2072.215 kb on + strandat 2072.215 kb on + strandat 2072.216 kb on - strandat 2072.216 kb on - strandat 2072.216 kb on - strandat 2072.262 kb on + strandat 2072.263 kb on - strandat 2072.263 kb on - strandat 2072.263 kb on - strandat 2072.263 kb on - strandat 2072.263 kb on - strandat 2072.266 kb on + strandat 2072.267 kb on - strandat 2072.267 kb on - strandat 2072.288 kb on + strandat 2072.289 kb on - strandat 2072.289 kb on - strandat 2072.686 kb on + strandat 2072.761 kb on - strandat 2073.189 kb on + strand, within Pf1N1B4_1805at 2073.190 kb on - strand, within Pf1N1B4_1805at 2073.210 kb on + strand, within Pf1N1B4_1805at 2073.210 kb on + strand, within Pf1N1B4_1805at 2073.210 kb on + strand, within Pf1N1B4_1805at 2073.210 kb on + strand, within Pf1N1B4_1805at 2073.210 kb on + strand, within Pf1N1B4_1805at 2073.211 kb on - strand, within Pf1N1B4_1805

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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2,070,642 + +0.0
2,070,642 + +0.0
2,070,643 - -3.1
2,070,643 - -2.3
2,070,643 - +0.0
2,070,643 - -2.9
2,070,643 - -1.1
2,070,643 - -1.8
2,070,643 - -3.1
2,070,643 - +0.0
2,070,646 + +0.0
2,070,646 + +0.0
2,070,647 - -1.8
2,070,647 - -2.3
2,070,647 - -3.1
2,070,647 - +0.0
2,070,647 - -2.2
2,070,781 - Pf1N1B4_1803 0.20 +0.0
2,070,871 - Pf1N1B4_1803 0.33 +0.0
2,070,871 - Pf1N1B4_1803 0.33 -1.9
2,070,871 - Pf1N1B4_1803 0.33 +0.0
2,070,873 + Pf1N1B4_1803 0.33 +0.0
2,070,873 + Pf1N1B4_1803 0.33 -1.2
2,070,873 + Pf1N1B4_1803 0.33 -1.0
2,070,873 + -1.6
2,070,874 - Pf1N1B4_1803 0.34 +0.0
2,070,918 + Pf1N1B4_1803 0.40 +0.0
2,070,919 - Pf1N1B4_1803 0.40 -3.4
2,070,919 - Pf1N1B4_1803 0.40 +0.0
2,070,919 - Pf1N1B4_1803 0.40 +0.0
2,070,969 + Pf1N1B4_1803 0.47 +0.0
2,070,969 + Pf1N1B4_1803 0.47 -1.3
2,070,970 - Pf1N1B4_1803 0.47 +0.0
2,070,984 + Pf1N1B4_1803 0.49 +0.0
2,070,985 - Pf1N1B4_1803 0.50 +0.0
2,071,013 + Pf1N1B4_1803 0.54 +0.0
2,071,200 + Pf1N1B4_1803 0.81 +0.0
2,071,201 - Pf1N1B4_1803 0.81 +0.0
2,071,201 - Pf1N1B4_1803 0.81 -2.6
2,071,201 - Pf1N1B4_1803 0.81 -0.4
2,071,429 - +0.0
2,071,436 - +0.0
2,071,436 - -2.5
2,071,436 - +0.0
2,071,441 - -0.1
2,071,441 - +0.0
2,071,441 - +0.0
2,071,508 - +0.0
2,071,508 - +0.0
2,071,562 + +0.0
2,071,563 - +0.0
2,071,699 + Pf1N1B4_1804 0.24 -3.8
2,071,754 + Pf1N1B4_1804 0.32 -2.1
2,071,754 + Pf1N1B4_1804 0.32 +0.0
2,071,754 + Pf1N1B4_1804 0.32 -2.1
2,071,755 - Pf1N1B4_1804 0.32 -3.3
2,071,755 - Pf1N1B4_1804 0.32 -3.2
2,071,755 - Pf1N1B4_1804 0.32 +0.0
2,071,818 + Pf1N1B4_1804 0.41 +0.0
2,071,818 + Pf1N1B4_1804 0.41 -2.7
2,071,818 + Pf1N1B4_1804 0.41 +0.0
2,071,818 + Pf1N1B4_1804 0.41 +0.0
2,071,818 + Pf1N1B4_1804 0.41 -3.4
2,071,819 - Pf1N1B4_1804 0.41 -2.9
2,071,819 - Pf1N1B4_1804 0.41 +0.0
2,071,819 - Pf1N1B4_1804 0.41 +0.0
2,071,905 + Pf1N1B4_1804 0.54 +0.0
2,071,905 + Pf1N1B4_1804 0.54 -3.5
2,071,906 - Pf1N1B4_1804 0.54 +0.0
2,071,906 - Pf1N1B4_1804 0.54 +0.0
2,071,906 - Pf1N1B4_1804 0.54 +0.0
2,071,906 - Pf1N1B4_1804 0.54 +0.0
2,071,906 - Pf1N1B4_1804 0.54 +0.0
2,071,906 - Pf1N1B4_1804 0.54 -3.3
2,071,908 - Pf1N1B4_1804 0.54 +0.0
2,071,981 - +0.0
2,071,995 - Pf1N1B4_1804 0.67 -2.6
2,071,995 - Pf1N1B4_1804 0.67 -2.2
2,072,199 + +0.0
2,072,215 + +0.0
2,072,215 + +0.0
2,072,215 + +0.0
2,072,216 - -2.3
2,072,216 - +0.0
2,072,216 - +0.0
2,072,262 + -1.5
2,072,263 - +0.0
2,072,263 - +0.0
2,072,263 - +0.0
2,072,263 - +0.0
2,072,263 - -2.2
2,072,266 + -2.2
2,072,267 - -3.2
2,072,267 - -1.2
2,072,288 + +0.0
2,072,289 - +0.0
2,072,289 - +0.0
2,072,686 + +0.0
2,072,761 - -4.2
2,073,189 + Pf1N1B4_1805 0.35 -1.7
2,073,190 - Pf1N1B4_1805 0.35 +0.0
2,073,210 + Pf1N1B4_1805 0.37 -2.0
2,073,210 + Pf1N1B4_1805 0.37 +0.0
2,073,210 + Pf1N1B4_1805 0.37 -1.7
2,073,210 + Pf1N1B4_1805 0.37 +0.0
2,073,210 + Pf1N1B4_1805 0.37 +0.0
2,073,211 - Pf1N1B4_1805 0.38 +0.0

Or see this region's nucleotide sequence