Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_15 and Pf1N1B4_16 are separated by 158 nucleotides Pf1N1B4_16 and Pf1N1B4_17 are separated by 105 nucleotides
Pf1N1B4_15 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 16,025 to 17,443
_15
Pf1N1B4_16 - Cys regulon transcriptional activator CysB, at 17,602 to 18,492
_16
Pf1N1B4_17 - Sensory box/GGDEF family protein, at 18,598 to 21,855
_17
Position (kb) 17
18
19 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.704 kb on + strand, within Pf1N1B4_15 at 16.774 kb on + strand, within Pf1N1B4_15 at 16.775 kb on - strand, within Pf1N1B4_15 at 17.165 kb on - strand, within Pf1N1B4_15 at 17.364 kb on + strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.365 kb on - strand at 17.471 kb on - strand at 17.823 kb on - strand, within Pf1N1B4_16 at 17.927 kb on + strand, within Pf1N1B4_16 at 17.931 kb on - strand, within Pf1N1B4_16 at 18.271 kb on + strand, within Pf1N1B4_16 at 18.272 kb on - strand at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.392 kb on + strand, within Pf1N1B4_16 at 18.398 kb on - strand at 18.398 kb on - strand, within Pf1N1B4_16 at 18.521 kb on + strand at 18.522 kb on - strand at 18.524 kb on - strand at 18.597 kb on + strand at 18.598 kb on - strand at 18.598 kb on - strand at 18.709 kb on + strand at 18.805 kb on + strand at 18.805 kb on + strand at 19.136 kb on - strand, within Pf1N1B4_17 at 19.136 kb on - strand, within Pf1N1B4_17 at 19.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 16,704 + Pf1N1B4_15 0.48 +0.0 16,704 + Pf1N1B4_15 0.48 +0.0 16,704 + Pf1N1B4_15 0.48 +0.0 16,774 + Pf1N1B4_15 0.53 +0.0 16,775 - Pf1N1B4_15 0.53 -4.2 17,165 - Pf1N1B4_15 0.80 -6.4 17,364 + -5.6 17,365 - +0.0 17,365 - -5.1 17,365 - +0.0 17,365 - -1.4 17,365 - +0.0 17,471 - +0.0 17,823 - Pf1N1B4_16 0.25 +0.0 17,927 + Pf1N1B4_16 0.36 +0.0 17,931 - Pf1N1B4_16 0.37 +0.0 18,271 + Pf1N1B4_16 0.75 +0.0 18,272 - +0.0 18,392 + Pf1N1B4_16 0.89 +0.0 18,392 + Pf1N1B4_16 0.89 -2.7 18,392 + Pf1N1B4_16 0.89 -3.4 18,398 - -1.2 18,398 - Pf1N1B4_16 0.89 -4.2 18,521 + +0.0 18,522 - -2.6 18,524 - -2.4 18,597 + -2.2 18,598 - +0.0 18,598 - +0.0 18,709 + +0.0 18,805 + +0.0 18,805 + -1.8 19,136 - Pf1N1B4_17 0.17 +0.0 19,136 - Pf1N1B4_17 0.17 +0.0 19,355 - +0.0
Or see this region's nucleotide sequence