Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotides Pf1N1B4_150 and Pf1N1B4_151 are separated by 42 nucleotides
Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797
_149
Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085
_150
Pf1N1B4_151 - Thioesterase in siderophore biosynthesis gene cluster, at 193,128 to 193,868
_151
Position (kb) 192
193
194 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.584 kb on - strand at 191.584 kb on - strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.638 kb on - strand at 191.883 kb on + strand at 191.906 kb on - strand at 192.305 kb on - strand, within Pf1N1B4_150 at 192.598 kb on + strand at 192.653 kb on - strand, within Pf1N1B4_150 at 192.653 kb on - strand, within Pf1N1B4_150 at 192.712 kb on + strand, within Pf1N1B4_150 at 192.713 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.848 kb on - strand, within Pf1N1B4_150 at 192.848 kb on - strand, within Pf1N1B4_150 at 193.111 kb on - strand at 193.190 kb on + strand at 193.813 kb on + strand at 193.813 kb on + strand at 193.875 kb on + strand at 193.894 kb on + strand at 193.895 kb on - strand at 193.925 kb on + strand at 193.959 kb on + strand at 193.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 191,193 + Pf1N1B4_149 0.82 +0.0 191,193 + Pf1N1B4_149 0.82 -3.6 191,194 - Pf1N1B4_149 0.82 +0.0 191,194 - Pf1N1B4_149 0.82 -5.1 191,584 - -1.3 191,584 - +0.0 191,637 + +0.0 191,637 + +0.0 191,637 + +0.0 191,638 - +0.0 191,883 + -2.0 191,906 - +0.0 192,305 - Pf1N1B4_150 0.21 -3.0 192,598 + -1.3 192,653 - Pf1N1B4_150 0.56 +0.0 192,653 - Pf1N1B4_150 0.56 +0.0 192,712 + Pf1N1B4_150 0.62 +0.0 192,713 - Pf1N1B4_150 0.62 -2.7 192,746 - Pf1N1B4_150 0.65 +0.0 192,746 - Pf1N1B4_150 0.65 -2.6 192,746 - Pf1N1B4_150 0.65 -4.6 192,848 - Pf1N1B4_150 0.76 +0.0 192,848 - Pf1N1B4_150 0.76 +0.0 193,111 - +0.0 193,190 + +0.0 193,813 + -2.5 193,813 + -2.4 193,875 + +0.0 193,894 + -1.7 193,895 - +0.0 193,925 + -2.2 193,959 + -2.6 193,960 - -1.7
Or see this region's nucleotide sequence