Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_135

Experiment: Nitrate reduction, replicate A t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_134 and Pf1N1B4_135 are separated by 0 nucleotidesPf1N1B4_135 and Pf1N1B4_136 are separated by 7 nucleotides Pf1N1B4_134 - pyoverdine-specific efflux macA-like protein, at 147,967 to 149,127 _134 Pf1N1B4_135 - Pyoverdine efflux carrier and ATP binding protein, at 149,128 to 151,101 _135 Pf1N1B4_136 - Outer membrane pyoverdine eflux protein, at 151,109 to 152,503 _136 Position (kb) 149 150 151 152Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 148.147 kb on + strand, within Pf1N1B4_134at 148.147 kb on + strand, within Pf1N1B4_134at 148.148 kb on - strand, within Pf1N1B4_134at 148.148 kb on - strand, within Pf1N1B4_134at 148.148 kb on - strand, within Pf1N1B4_134at 148.148 kb on - strand, within Pf1N1B4_134at 148.241 kb on + strand, within Pf1N1B4_134at 148.241 kb on + strand, within Pf1N1B4_134at 148.241 kb on + strand, within Pf1N1B4_134at 148.241 kb on + strand, within Pf1N1B4_134at 148.242 kb on - strand, within Pf1N1B4_134at 148.352 kb on - strand, within Pf1N1B4_134at 148.526 kb on - strand, within Pf1N1B4_134at 148.526 kb on - strand, within Pf1N1B4_134at 148.526 kb on - strand, within Pf1N1B4_134at 148.834 kb on + strand, within Pf1N1B4_134at 148.899 kb on - strand, within Pf1N1B4_134at 148.899 kb on - strand, within Pf1N1B4_134at 149.326 kb on + strand, within Pf1N1B4_135at 149.470 kb on + strand, within Pf1N1B4_135at 149.470 kb on + strand, within Pf1N1B4_135at 149.924 kb on + strand, within Pf1N1B4_135at 149.924 kb on + strand, within Pf1N1B4_135at 150.129 kb on + strand, within Pf1N1B4_135at 150.129 kb on + strand, within Pf1N1B4_135at 150.256 kb on + strand, within Pf1N1B4_135at 150.256 kb on + strand, within Pf1N1B4_135at 150.256 kb on + strand, within Pf1N1B4_135at 150.257 kb on - strand, within Pf1N1B4_135at 150.319 kb on + strand, within Pf1N1B4_135at 150.320 kb on - strandat 150.524 kb on - strand, within Pf1N1B4_135at 151.108 kb on + strandat 151.109 kb on - strandat 151.448 kb on + strand, within Pf1N1B4_136at 151.490 kb on + strand, within Pf1N1B4_136at 151.494 kb on - strand, within Pf1N1B4_136at 151.640 kb on + strand, within Pf1N1B4_136at 151.641 kb on - strand, within Pf1N1B4_136at 151.641 kb on - strand, within Pf1N1B4_136at 151.641 kb on - strand, within Pf1N1B4_136

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2
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148,147 + Pf1N1B4_134 0.16 -2.5
148,147 + Pf1N1B4_134 0.16 -1.4
148,148 - Pf1N1B4_134 0.16 -3.4
148,148 - Pf1N1B4_134 0.16 +0.0
148,148 - Pf1N1B4_134 0.16 +0.0
148,148 - Pf1N1B4_134 0.16 -2.8
148,241 + Pf1N1B4_134 0.24 +0.0
148,241 + Pf1N1B4_134 0.24 -1.3
148,241 + Pf1N1B4_134 0.24 +0.0
148,241 + Pf1N1B4_134 0.24 +0.0
148,242 - Pf1N1B4_134 0.24 +0.0
148,352 - Pf1N1B4_134 0.33 +0.0
148,526 - Pf1N1B4_134 0.48 +0.0
148,526 - Pf1N1B4_134 0.48 +0.0
148,526 - Pf1N1B4_134 0.48 -3.8
148,834 + Pf1N1B4_134 0.75 -1.8
148,899 - Pf1N1B4_134 0.80 -1.8
148,899 - Pf1N1B4_134 0.80 +0.0
149,326 + Pf1N1B4_135 0.10 +0.0
149,470 + Pf1N1B4_135 0.17 +0.0
149,470 + Pf1N1B4_135 0.17 +0.0
149,924 + Pf1N1B4_135 0.40 +0.0
149,924 + Pf1N1B4_135 0.40 -0.5
150,129 + Pf1N1B4_135 0.51 +0.0
150,129 + Pf1N1B4_135 0.51 +0.0
150,256 + Pf1N1B4_135 0.57 -1.8
150,256 + Pf1N1B4_135 0.57 +0.0
150,256 + Pf1N1B4_135 0.57 +0.0
150,257 - Pf1N1B4_135 0.57 +0.0
150,319 + Pf1N1B4_135 0.60 +0.0
150,320 - +0.0
150,524 - Pf1N1B4_135 0.71 -1.4
151,108 + -2.5
151,109 - -2.6
151,448 + Pf1N1B4_136 0.24 -1.3
151,490 + Pf1N1B4_136 0.27 +0.0
151,494 - Pf1N1B4_136 0.28 -3.1
151,640 + Pf1N1B4_136 0.38 +0.0
151,641 - Pf1N1B4_136 0.38 -3.2
151,641 - Pf1N1B4_136 0.38 +0.0
151,641 - Pf1N1B4_136 0.38 -2.6

Or see this region's nucleotide sequence