Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_124 and Pf1N1B4_125 are separated by 189 nucleotides Pf1N1B4_125 and Pf1N1B4_126 are separated by 127 nucleotides
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Pf1N1B4_125 - Nicotinamidase family protein YcaC, at 137,213 to 137,839
_125
Pf1N1B4_126 - Pirin, at 137,967 to 138,833
_126
Position (kb) 137
138 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 137.040 kb on + strand at 137.041 kb on - strand at 137.043 kb on + strand at 137.044 kb on - strand at 137.044 kb on - strand at 137.060 kb on + strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.128 kb on - strand at 137.189 kb on + strand at 137.189 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.214 kb on + strand at 137.215 kb on - strand at 137.238 kb on + strand at 137.239 kb on - strand at 137.239 kb on - strand at 137.695 kb on - strand, within Pf1N1B4_125 at 137.834 kb on - strand at 137.834 kb on - strand at 137.924 kb on + strand at 137.955 kb on - strand at 137.955 kb on - strand at 138.289 kb on - strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.811 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 136,647 + Pf1N1B4_124 0.74 +0.0 136,647 + Pf1N1B4_124 0.74 +0.0 136,648 - Pf1N1B4_124 0.74 +0.0 136,648 - Pf1N1B4_124 0.74 +0.0 137,040 + +0.0 137,041 - +0.0 137,043 + -1.9 137,044 - -1.7 137,044 - -2.5 137,060 + +0.0 137,061 - -3.3 137,061 - +0.0 137,061 - +0.0 137,128 - +0.0 137,189 + +0.0 137,189 + +0.0 137,197 + -3.2 137,197 + +0.0 137,197 + +0.0 137,197 + -3.3 137,197 + +0.0 137,198 - +0.0 137,198 - +0.0 137,198 - -3.7 137,198 - -4.7 137,198 - +0.0 137,214 + +0.0 137,215 - -2.6 137,238 + -1.0 137,239 - +0.0 137,239 - +0.0 137,695 - Pf1N1B4_125 0.77 -1.9 137,834 - -1.0 137,834 - -0.8 137,924 + -2.2 137,955 - +0.0 137,955 - +0.0 138,289 - Pf1N1B4_126 0.37 +0.0 138,616 + Pf1N1B4_126 0.75 -3.8 138,616 + Pf1N1B4_126 0.75 +0.0 138,616 + Pf1N1B4_126 0.75 -2.6 138,811 + +0.0
Or see this region's nucleotide sequence