Experiment: Nitrate reduction, replicate A t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_116 and Pf1N1B4_117 are separated by 88 nucleotides Pf1N1B4_117 and Pf1N1B4_118 are separated by 40 nucleotides Pf1N1B4_118 and Pf1N1B4_119 are separated by 277 nucleotides
Pf1N1B4_116 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 121,293 to 123,725
_116
Pf1N1B4_117 - Iron siderophore sensor protein, at 123,814 to 124,788
_117
Pf1N1B4_118 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis @ FIG006045: Sigma factor, ECF subfamily, at 124,829 to 125,329
_118
Pf1N1B4_119 - Putative haemolysin/cytolysin secreted via TPS pathway, at 125,607 to 130,118
_119
Position (kb) 123
124
125 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 122.818 kb on + strand, within Pf1N1B4_116 at 122.819 kb on - strand, within Pf1N1B4_116 at 122.819 kb on - strand, within Pf1N1B4_116 at 123.609 kb on - strand at 123.786 kb on - strand at 123.786 kb on - strand at 123.826 kb on + strand at 123.826 kb on + strand at 123.826 kb on + strand at 123.827 kb on - strand at 123.827 kb on - strand at 124.074 kb on + strand, within Pf1N1B4_117 at 124.074 kb on + strand at 124.076 kb on + strand, within Pf1N1B4_117 at 124.077 kb on - strand, within Pf1N1B4_117 at 124.246 kb on - strand, within Pf1N1B4_117 at 124.436 kb on + strand, within Pf1N1B4_117 at 124.437 kb on - strand, within Pf1N1B4_117 at 124.448 kb on + strand, within Pf1N1B4_117 at 124.449 kb on - strand, within Pf1N1B4_117 at 124.505 kb on - strand, within Pf1N1B4_117 at 124.505 kb on - strand, within Pf1N1B4_117 at 124.839 kb on - strand at 124.839 kb on - strand at 124.839 kb on - strand at 124.883 kb on - strand at 124.883 kb on - strand, within Pf1N1B4_118 at 124.883 kb on - strand, within Pf1N1B4_118 at 124.884 kb on + strand, within Pf1N1B4_118 at 124.884 kb on + strand, within Pf1N1B4_118 at 124.884 kb on + strand, within Pf1N1B4_118 at 124.885 kb on - strand, within Pf1N1B4_118 at 124.885 kb on - strand, within Pf1N1B4_118 at 124.885 kb on - strand at 125.406 kb on + strand at 125.406 kb on + strand at 125.407 kb on - strand at 125.407 kb on - strand at 125.437 kb on + strand at 125.441 kb on + strand at 125.441 kb on + strand at 125.441 kb on + strand at 125.442 kb on - strand at 125.456 kb on + strand at 125.456 kb on + strand at 125.456 kb on + strand at 125.457 kb on - strand at 125.478 kb on + strand at 125.484 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.608 kb on + strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.609 kb on - strand at 125.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t2 remove 122,818 + Pf1N1B4_116 0.63 +0.0 122,819 - Pf1N1B4_116 0.63 +0.0 122,819 - Pf1N1B4_116 0.63 +0.0 123,609 - +0.0 123,786 - -2.8 123,786 - +0.0 123,826 + -2.0 123,826 + +0.0 123,826 + -2.9 123,827 - +0.0 123,827 - -2.2 124,074 + Pf1N1B4_117 0.27 +0.0 124,074 + +0.0 124,076 + Pf1N1B4_117 0.27 -2.6 124,077 - Pf1N1B4_117 0.27 +0.0 124,246 - Pf1N1B4_117 0.44 -3.1 124,436 + Pf1N1B4_117 0.64 +0.0 124,437 - Pf1N1B4_117 0.64 +0.0 124,448 + Pf1N1B4_117 0.65 -3.2 124,449 - Pf1N1B4_117 0.65 -2.5 124,505 - Pf1N1B4_117 0.71 -1.9 124,505 - Pf1N1B4_117 0.71 -3.9 124,839 - +0.0 124,839 - +0.0 124,839 - +0.0 124,883 - -1.1 124,883 - Pf1N1B4_118 0.11 +0.0 124,883 - Pf1N1B4_118 0.11 +0.0 124,884 + Pf1N1B4_118 0.11 +0.0 124,884 + Pf1N1B4_118 0.11 -2.6 124,884 + Pf1N1B4_118 0.11 +0.0 124,885 - Pf1N1B4_118 0.11 +0.0 124,885 - Pf1N1B4_118 0.11 +0.0 124,885 - -1.3 125,406 + +0.0 125,406 + -2.7 125,407 - +0.0 125,407 - +0.0 125,437 + +0.0 125,441 + +0.0 125,441 + +0.0 125,441 + +0.0 125,442 - +0.0 125,456 + +0.0 125,456 + -1.5 125,456 + +0.0 125,457 - +0.0 125,478 + +0.0 125,484 + -0.5 125,608 + -1.1 125,608 + +0.0 125,608 + -2.9 125,608 + +0.0 125,608 + -2.7 125,609 - -3.4 125,609 - +0.0 125,609 - +0.0 125,609 - +0.0 125,609 - +0.0 125,720 + +0.0
Or see this region's nucleotide sequence