Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT_t28

Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1591 and tRNA-Cys are separated by 488 nucleotidestRNA-Cys and BT1592 are separated by 263 nucleotides BT1591: BT1591 - hypothetical protein (NCBI ptt file), at 1,952,372 to 1,955,104 BT1591 BT_t28: tRNA-Cys - tRNA-Cys (RefSeq), at 1,955,593 to 1,955,664 tRNA-Cys BT1592: BT1592 - putative ferrous iron transport protein (NCBI ptt file), at 1,955,928 to 1,958,414 BT1592 Position (kb) 1955 1956Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1954.772 kb on + strand, within BT1591at 1954.773 kb on - strand, within BT1591at 1954.773 kb on - strand, within BT1591at 1954.777 kb on - strand, within BT1591at 1954.777 kb on - strand, within BT1591at 1954.800 kb on - strand, within BT1591at 1954.854 kb on + strandat 1954.855 kb on - strandat 1954.960 kb on + strandat 1954.964 kb on - strandat 1954.966 kb on - strandat 1955.011 kb on + strandat 1955.011 kb on + strandat 1955.012 kb on - strandat 1955.155 kb on - strandat 1955.231 kb on + strandat 1955.347 kb on - strandat 1955.398 kb on + strandat 1955.398 kb on + strandat 1955.497 kb on - strandat 1955.504 kb on + strandat 1955.784 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM
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1,954,772 + BT1591 0.88 +0.4
1,954,773 - BT1591 0.88 -0.6
1,954,773 - BT1591 0.88 -1.9
1,954,777 - BT1591 0.88 +0.7
1,954,777 - BT1591 0.88 -0.6
1,954,800 - BT1591 0.89 +0.4
1,954,854 + -4.0
1,954,855 - +1.3
1,954,960 + +1.0
1,954,964 - -1.6
1,954,966 - -2.1
1,955,011 + +0.4
1,955,011 + -2.6
1,955,012 - -2.7
1,955,155 - -0.2
1,955,231 + -1.1
1,955,347 - -0.6
1,955,398 + -0.9
1,955,398 + +0.4
1,955,497 - -2.1
1,955,504 + -1.9
1,955,784 + +2.0

Or see this region's nucleotide sequence