Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3311 and BT3312 are separated by 58 nucleotides BT3312 and BT3313 are separated by 22 nucleotides
BT3311: BT3311 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,251,580 to 4,253,100
BT3311
BT3312: BT3312 - glucosylceramidase precursor (NCBI ptt file), at 4,253,159 to 4,254,649
BT3312
BT3313: BT3313 - hypothetical protein (NCBI ptt file), at 4,254,672 to 4,256,675
BT3313
Position (kb)
4253
4254
4255 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4252.438 kb on - strand, within BT3311 at 4252.622 kb on + strand, within BT3311 at 4252.622 kb on + strand, within BT3311 at 4252.627 kb on + strand, within BT3311 at 4252.630 kb on + strand, within BT3311 at 4252.631 kb on - strand, within BT3311 at 4252.701 kb on + strand, within BT3311 at 4252.713 kb on + strand, within BT3311 at 4252.739 kb on + strand, within BT3311 at 4252.794 kb on + strand, within BT3311 at 4252.956 kb on - strand at 4252.976 kb on + strand at 4252.976 kb on + strand at 4252.978 kb on + strand at 4253.124 kb on - strand at 4253.124 kb on - strand at 4253.126 kb on - strand at 4253.163 kb on + strand at 4253.176 kb on + strand at 4253.209 kb on - strand at 4253.227 kb on + strand at 4253.330 kb on - strand, within BT3312 at 4253.419 kb on + strand, within BT3312 at 4253.420 kb on - strand, within BT3312 at 4253.431 kb on + strand, within BT3312 at 4253.523 kb on - strand, within BT3312 at 4253.523 kb on - strand, within BT3312 at 4253.567 kb on + strand, within BT3312 at 4253.569 kb on + strand, within BT3312 at 4253.569 kb on + strand, within BT3312 at 4253.570 kb on - strand, within BT3312 at 4253.628 kb on - strand, within BT3312 at 4253.629 kb on + strand, within BT3312 at 4253.679 kb on + strand, within BT3312 at 4253.780 kb on + strand, within BT3312 at 4253.781 kb on - strand, within BT3312 at 4253.793 kb on - strand, within BT3312 at 4253.802 kb on - strand, within BT3312 at 4253.850 kb on - strand, within BT3312 at 4253.850 kb on - strand, within BT3312 at 4253.857 kb on - strand, within BT3312 at 4253.903 kb on + strand, within BT3312 at 4254.011 kb on + strand, within BT3312 at 4254.039 kb on - strand, within BT3312 at 4254.100 kb on + strand, within BT3312 at 4254.135 kb on + strand, within BT3312 at 4254.136 kb on - strand, within BT3312 at 4254.136 kb on - strand, within BT3312 at 4254.136 kb on - strand, within BT3312 at 4254.146 kb on + strand, within BT3312 at 4254.181 kb on + strand, within BT3312 at 4254.232 kb on + strand, within BT3312 at 4254.243 kb on + strand, within BT3312 at 4254.244 kb on - strand, within BT3312 at 4254.271 kb on + strand, within BT3312 at 4254.272 kb on - strand, within BT3312 at 4254.279 kb on + strand, within BT3312 at 4254.279 kb on + strand, within BT3312 at 4254.280 kb on - strand, within BT3312 at 4254.347 kb on + strand, within BT3312 at 4254.357 kb on + strand, within BT3312 at 4254.378 kb on - strand, within BT3312 at 4254.390 kb on + strand, within BT3312 at 4254.405 kb on - strand, within BT3312 at 4254.521 kb on + strand at 4254.565 kb on - strand at 4254.657 kb on - strand at 4254.688 kb on - strand at 4254.688 kb on - strand at 4254.690 kb on - strand at 4254.690 kb on - strand at 4254.691 kb on + strand at 4254.692 kb on - strand at 4254.692 kb on - strand at 4254.933 kb on + strand, within BT3313 at 4254.936 kb on - strand, within BT3313 at 4254.958 kb on - strand, within BT3313 at 4254.961 kb on - strand, within BT3313 at 4254.961 kb on - strand, within BT3313 at 4254.968 kb on + strand, within BT3313 at 4255.001 kb on - strand, within BT3313 at 4255.010 kb on + strand, within BT3313 at 4255.019 kb on + strand, within BT3313 at 4255.019 kb on + strand, within BT3313 at 4255.084 kb on + strand at 4255.129 kb on - strand, within BT3313 at 4255.129 kb on - strand, within BT3313 at 4255.134 kb on + strand, within BT3313 at 4255.136 kb on + strand, within BT3313 at 4255.175 kb on + strand, within BT3313 at 4255.256 kb on + strand, within BT3313 at 4255.263 kb on - strand, within BT3313 at 4255.294 kb on - strand, within BT3313 at 4255.308 kb on - strand, within BT3313 at 4255.320 kb on + strand, within BT3313 at 4255.387 kb on + strand, within BT3313 at 4255.388 kb on - strand, within BT3313 at 4255.389 kb on + strand, within BT3313 at 4255.449 kb on + strand, within BT3313 at 4255.539 kb on - strand, within BT3313 at 4255.561 kb on - strand, within BT3313 at 4255.561 kb on - strand, within BT3313 at 4255.573 kb on + strand, within BT3313 at 4255.574 kb on - strand, within BT3313 at 4255.626 kb on - strand, within BT3313
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM remove 4,252,438 - BT3311 0.56 -1.2 4,252,622 + BT3311 0.69 -2.0 4,252,622 + BT3311 0.69 +0.6 4,252,627 + BT3311 0.69 -0.7 4,252,630 + BT3311 0.69 -2.5 4,252,631 - BT3311 0.69 -0.7 4,252,701 + BT3311 0.74 -2.7 4,252,713 + BT3311 0.74 -1.7 4,252,739 + BT3311 0.76 +0.3 4,252,794 + BT3311 0.80 -2.2 4,252,956 - +1.0 4,252,976 + -1.2 4,252,976 + -0.4 4,252,978 + -0.7 4,253,124 - -3.9 4,253,124 - -3.0 4,253,126 - -1.7 4,253,163 + -0.9 4,253,176 + -1.2 4,253,209 - +0.3 4,253,227 + -1.1 4,253,330 - BT3312 0.11 -2.5 4,253,419 + BT3312 0.17 -1.2 4,253,420 - BT3312 0.18 -0.3 4,253,431 + BT3312 0.18 -3.7 4,253,523 - BT3312 0.24 +0.3 4,253,523 - BT3312 0.24 -1.2 4,253,567 + BT3312 0.27 -1.7 4,253,569 + BT3312 0.27 -1.3 4,253,569 + BT3312 0.27 +1.1 4,253,570 - BT3312 0.28 +0.3 4,253,628 - BT3312 0.31 -4.1 4,253,629 + BT3312 0.32 -2.7 4,253,679 + BT3312 0.35 +0.3 4,253,780 + BT3312 0.42 -0.2 4,253,781 - BT3312 0.42 -1.1 4,253,793 - BT3312 0.43 -2.2 4,253,802 - BT3312 0.43 -1.2 4,253,850 - BT3312 0.46 -1.2 4,253,850 - BT3312 0.46 +0.6 4,253,857 - BT3312 0.47 +0.3 4,253,903 + BT3312 0.50 +0.2 4,254,011 + BT3312 0.57 -3.0 4,254,039 - BT3312 0.59 -0.7 4,254,100 + BT3312 0.63 +1.6 4,254,135 + BT3312 0.65 -1.2 4,254,136 - BT3312 0.66 +0.1 4,254,136 - BT3312 0.66 +0.3 4,254,136 - BT3312 0.66 +0.3 4,254,146 + BT3312 0.66 +1.9 4,254,181 + BT3312 0.69 -0.6 4,254,232 + BT3312 0.72 -0.7 4,254,243 + BT3312 0.73 +0.3 4,254,244 - BT3312 0.73 -0.2 4,254,271 + BT3312 0.75 +0.3 4,254,272 - BT3312 0.75 +0.1 4,254,279 + BT3312 0.75 +0.3 4,254,279 + BT3312 0.75 -1.2 4,254,280 - BT3312 0.75 -1.7 4,254,347 + BT3312 0.80 -2.7 4,254,357 + BT3312 0.80 +0.3 4,254,378 - BT3312 0.82 -2.0 4,254,390 + BT3312 0.83 -2.0 4,254,405 - BT3312 0.84 +0.8 4,254,521 + -1.7 4,254,565 - -0.7 4,254,657 - +0.9 4,254,688 - +1.9 4,254,688 - -0.7 4,254,690 - +1.9 4,254,690 - +1.2 4,254,691 + -0.7 4,254,692 - -0.2 4,254,692 - +0.7 4,254,933 + BT3313 0.13 -0.7 4,254,936 - BT3313 0.13 -0.7 4,254,958 - BT3313 0.14 -3.8 4,254,961 - BT3313 0.14 -1.2 4,254,961 - BT3313 0.14 -2.2 4,254,968 + BT3313 0.15 -0.6 4,255,001 - BT3313 0.16 -0.7 4,255,010 + BT3313 0.17 +0.3 4,255,019 + BT3313 0.17 -0.2 4,255,019 + BT3313 0.17 +0.9 4,255,084 + +0.3 4,255,129 - BT3313 0.23 -2.0 4,255,129 - BT3313 0.23 -2.8 4,255,134 + BT3313 0.23 +0.3 4,255,136 + BT3313 0.23 +0.3 4,255,175 + BT3313 0.25 -1.2 4,255,256 + BT3313 0.29 -2.2 4,255,263 - BT3313 0.29 -1.7 4,255,294 - BT3313 0.31 -1.3 4,255,308 - BT3313 0.32 -1.2 4,255,320 + BT3313 0.32 -2.5 4,255,387 + BT3313 0.36 -1.2 4,255,388 - BT3313 0.36 -3.0 4,255,389 + BT3313 0.36 -1.2 4,255,449 + BT3313 0.39 -2.0 4,255,539 - BT3313 0.43 -2.0 4,255,561 - BT3313 0.44 -1.7 4,255,561 - BT3313 0.44 +0.3 4,255,573 + BT3313 0.45 -1.2 4,255,574 - BT3313 0.45 +0.3 4,255,626 - BT3313 0.48 -3.1
Or see this region's nucleotide sequence