Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2681 and BT2682 overlap by 14 nucleotides BT2682 and BT2683 are separated by 28 nucleotides BT2683 and BT2684 are separated by 24 nucleotides BT2684 and BT2685 overlap by 4 nucleotides BT2685 and BT2686 are separated by 12 nucleotides
BT2681: BT2681 - alginate O-acetylation protein (NCBI ptt file), at 3,341,531 to 3,343,018
BT2681
BT2682: BT2682 - putative periplasmic protein (NCBI ptt file), at 3,343,005 to 3,343,871
BT2682
BT2683: BT2683 - putative periplasmic protein (NCBI ptt file), at 3,343,900 to 3,345,294
BT2683
BT2684: BT2684 - hypothetical protein (NCBI ptt file), at 3,345,319 to 3,345,519
BT2684
BT2685: BT2685 - hypothetical protein (NCBI ptt file), at 3,345,516 to 3,345,809
BT2685
BT2686: BT2686 - putative cation efflux transporter (NCBI ptt file), at 3,345,822 to 3,348,980
BT2686
Position (kb)
3343
3344
3345
3346 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3342.934 kb on + strand at 3342.973 kb on + strand at 3342.973 kb on + strand at 3342.973 kb on + strand at 3342.974 kb on - strand at 3342.974 kb on - strand at 3343.018 kb on + strand at 3343.027 kb on + strand at 3343.027 kb on + strand at 3343.051 kb on - strand at 3343.070 kb on + strand at 3343.073 kb on - strand at 3343.073 kb on - strand at 3343.158 kb on + strand, within BT2682 at 3343.159 kb on - strand, within BT2682 at 3343.166 kb on - strand, within BT2682 at 3343.367 kb on + strand, within BT2682 at 3343.369 kb on + strand, within BT2682 at 3343.512 kb on - strand, within BT2682 at 3343.517 kb on + strand, within BT2682 at 3343.518 kb on - strand, within BT2682 at 3343.519 kb on + strand, within BT2682 at 3343.611 kb on - strand, within BT2682 at 3343.642 kb on - strand, within BT2682 at 3343.692 kb on + strand, within BT2682 at 3343.692 kb on + strand, within BT2682 at 3343.751 kb on + strand, within BT2682 at 3343.884 kb on - strand at 3343.902 kb on - strand at 3343.914 kb on - strand at 3343.931 kb on + strand at 3343.932 kb on - strand at 3343.947 kb on - strand at 3343.948 kb on + strand at 3343.949 kb on - strand at 3343.949 kb on - strand at 3343.961 kb on + strand at 3344.007 kb on + strand at 3344.007 kb on + strand at 3344.008 kb on - strand at 3344.184 kb on + strand, within BT2683 at 3344.186 kb on + strand, within BT2683 at 3344.187 kb on - strand, within BT2683 at 3344.281 kb on - strand, within BT2683 at 3344.343 kb on - strand, within BT2683 at 3344.449 kb on - strand, within BT2683 at 3344.468 kb on + strand, within BT2683 at 3344.478 kb on + strand, within BT2683 at 3344.574 kb on + strand, within BT2683 at 3344.575 kb on - strand, within BT2683 at 3344.652 kb on + strand, within BT2683 at 3344.652 kb on + strand, within BT2683 at 3344.653 kb on - strand, within BT2683 at 3344.653 kb on - strand, within BT2683 at 3344.669 kb on + strand, within BT2683 at 3344.670 kb on - strand, within BT2683 at 3344.696 kb on + strand, within BT2683 at 3344.696 kb on + strand, within BT2683 at 3344.697 kb on - strand, within BT2683 at 3344.721 kb on - strand, within BT2683 at 3344.721 kb on - strand, within BT2683 at 3344.733 kb on + strand, within BT2683 at 3344.756 kb on + strand, within BT2683 at 3344.840 kb on - strand, within BT2683 at 3344.922 kb on + strand, within BT2683 at 3344.947 kb on - strand, within BT2683 at 3344.950 kb on + strand, within BT2683 at 3344.950 kb on + strand, within BT2683 at 3344.986 kb on - strand, within BT2683 at 3345.039 kb on - strand, within BT2683 at 3345.104 kb on - strand, within BT2683 at 3345.267 kb on - strand at 3345.276 kb on - strand at 3345.297 kb on + strand at 3345.300 kb on - strand at 3345.310 kb on - strand at 3345.466 kb on + strand, within BT2684 at 3345.471 kb on + strand, within BT2684 at 3345.478 kb on - strand, within BT2684 at 3345.553 kb on - strand, within BT2685 at 3345.574 kb on - strand, within BT2685 at 3345.594 kb on - strand, within BT2685 at 3345.615 kb on - strand, within BT2685 at 3345.650 kb on + strand, within BT2685 at 3345.651 kb on - strand, within BT2685 at 3345.810 kb on - strand at 3345.846 kb on - strand at 3345.891 kb on - strand at 3345.891 kb on - strand at 3345.965 kb on + strand at 3346.067 kb on + strand at 3346.067 kb on + strand at 3346.162 kb on + strand, within BT2686 at 3346.179 kb on - strand, within BT2686 at 3346.230 kb on + strand, within BT2686
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM remove 3,342,934 + -2.2 3,342,973 + -1.6 3,342,973 + +0.4 3,342,973 + -0.6 3,342,974 - -0.6 3,342,974 - -1.2 3,343,018 + -3.8 3,343,027 + -1.6 3,343,027 + +0.1 3,343,051 - -1.2 3,343,070 + -4.0 3,343,073 - +0.7 3,343,073 - -0.5 3,343,158 + BT2682 0.18 -1.2 3,343,159 - BT2682 0.18 -3.6 3,343,166 - BT2682 0.19 -0.3 3,343,367 + BT2682 0.42 -0.8 3,343,369 + BT2682 0.42 -0.6 3,343,512 - BT2682 0.58 +0.4 3,343,517 + BT2682 0.59 +0.4 3,343,518 - BT2682 0.59 +0.8 3,343,519 + BT2682 0.59 -2.2 3,343,611 - BT2682 0.70 +0.4 3,343,642 - BT2682 0.73 -0.6 3,343,692 + BT2682 0.79 +0.4 3,343,692 + BT2682 0.79 -0.1 3,343,751 + BT2682 0.86 -0.0 3,343,884 - +0.0 3,343,902 - -0.4 3,343,914 - -4.2 3,343,931 + -2.2 3,343,932 - -2.2 3,343,947 - -1.6 3,343,948 + -0.6 3,343,949 - -3.1 3,343,949 - -0.2 3,343,961 + -0.6 3,344,007 + -5.0 3,344,007 + +0.4 3,344,008 - -0.4 3,344,184 + BT2683 0.20 -0.6 3,344,186 + BT2683 0.21 -2.1 3,344,187 - BT2683 0.21 -2.5 3,344,281 - BT2683 0.27 -3.2 3,344,343 - BT2683 0.32 +0.4 3,344,449 - BT2683 0.39 -3.2 3,344,468 + BT2683 0.41 -4.5 3,344,478 + BT2683 0.41 -1.6 3,344,574 + BT2683 0.48 -1.6 3,344,575 - BT2683 0.48 +0.4 3,344,652 + BT2683 0.54 -2.4 3,344,652 + BT2683 0.54 -0.5 3,344,653 - BT2683 0.54 -2.6 3,344,653 - BT2683 0.54 -0.6 3,344,669 + BT2683 0.55 +0.4 3,344,670 - BT2683 0.55 -3.7 3,344,696 + BT2683 0.57 -1.4 3,344,696 + BT2683 0.57 +0.4 3,344,697 - BT2683 0.57 +0.4 3,344,721 - BT2683 0.59 -4.1 3,344,721 - BT2683 0.59 -0.6 3,344,733 + BT2683 0.60 -3.0 3,344,756 + BT2683 0.61 +0.4 3,344,840 - BT2683 0.67 -0.6 3,344,922 + BT2683 0.73 -2.0 3,344,947 - BT2683 0.75 +0.3 3,344,950 + BT2683 0.75 +0.4 3,344,950 + BT2683 0.75 -4.2 3,344,986 - BT2683 0.78 -3.0 3,345,039 - BT2683 0.82 +0.4 3,345,104 - BT2683 0.86 -1.6 3,345,267 - -2.4 3,345,276 - -3.2 3,345,297 + -4.0 3,345,300 - -0.6 3,345,310 - +0.3 3,345,466 + BT2684 0.73 -1.2 3,345,471 + BT2684 0.76 -0.4 3,345,478 - BT2684 0.79 +2.4 3,345,553 - BT2685 0.13 -4.1 3,345,574 - BT2685 0.20 +0.7 3,345,594 - BT2685 0.27 -2.0 3,345,615 - BT2685 0.34 +2.5 3,345,650 + BT2685 0.46 -1.2 3,345,651 - BT2685 0.46 -1.6 3,345,810 - +1.4 3,345,846 - -2.2 3,345,891 - -0.6 3,345,891 - -1.2 3,345,965 + -1.0 3,346,067 + -0.6 3,346,067 + +0.4 3,346,162 + BT2686 0.11 -0.6 3,346,179 - BT2686 0.11 +0.4 3,346,230 + BT2686 0.13 -1.2
Or see this region's nucleotide sequence