Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2212 and BT2213 are separated by 11 nucleotides BT2213 and BT2214 overlap by 4 nucleotides BT2214 and BT2215 are separated by 20 nucleotides
BT2212: BT2212 - magnesium chelatase, subunit I, putative ATPase (NCBI ptt file), at 2,780,158 to 2,781,132
BT2212
BT2213: BT2213 - hypothetical protein (NCBI ptt file), at 2,781,144 to 2,782,418
BT2213
BT2214: BT2214 - hypothetical protein (NCBI ptt file), at 2,782,415 to 2,783,029
BT2214
BT2215: BT2215 - hypothetical protein (NCBI ptt file), at 2,783,050 to 2,783,982
BT2215
Position (kb)
2781
2782
2783 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2780.379 kb on - strand, within BT2212 at 2780.380 kb on + strand, within BT2212 at 2780.424 kb on - strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.443 kb on + strand, within BT2212 at 2780.524 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.582 kb on + strand, within BT2212 at 2780.676 kb on + strand, within BT2212 at 2780.681 kb on + strand, within BT2212 at 2780.682 kb on - strand, within BT2212 at 2780.698 kb on - strand, within BT2212 at 2780.719 kb on + strand, within BT2212 at 2780.720 kb on - strand, within BT2212 at 2780.795 kb on - strand, within BT2212 at 2780.848 kb on - strand, within BT2212 at 2780.987 kb on - strand, within BT2212 at 2781.132 kb on + strand at 2781.132 kb on + strand at 2781.136 kb on + strand at 2781.146 kb on - strand at 2781.146 kb on - strand at 2781.180 kb on - strand at 2781.367 kb on + strand, within BT2213 at 2781.438 kb on - strand, within BT2213 at 2781.503 kb on + strand, within BT2213 at 2781.503 kb on + strand, within BT2213 at 2781.541 kb on + strand, within BT2213 at 2781.542 kb on - strand, within BT2213 at 2781.617 kb on + strand, within BT2213 at 2781.617 kb on + strand, within BT2213 at 2781.619 kb on + strand at 2781.676 kb on + strand, within BT2213 at 2781.787 kb on + strand, within BT2213 at 2781.788 kb on - strand, within BT2213 at 2781.788 kb on - strand, within BT2213 at 2781.968 kb on - strand, within BT2213 at 2782.034 kb on - strand, within BT2213 at 2782.055 kb on + strand, within BT2213 at 2782.055 kb on + strand, within BT2213 at 2782.100 kb on + strand, within BT2213 at 2782.100 kb on + strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.101 kb on - strand, within BT2213 at 2782.134 kb on + strand, within BT2213 at 2782.134 kb on + strand, within BT2213 at 2782.135 kb on - strand, within BT2213 at 2782.135 kb on - strand, within BT2213 at 2782.184 kb on + strand, within BT2213 at 2782.238 kb on + strand, within BT2213 at 2782.239 kb on - strand, within BT2213 at 2782.330 kb on - strand at 2782.356 kb on + strand at 2782.560 kb on + strand, within BT2214 at 2782.576 kb on + strand, within BT2214 at 2782.577 kb on - strand, within BT2214 at 2782.578 kb on + strand, within BT2214 at 2782.742 kb on - strand, within BT2214 at 2782.763 kb on + strand, within BT2214 at 2782.764 kb on - strand, within BT2214 at 2782.764 kb on - strand, within BT2214 at 2782.781 kb on - strand at 2782.813 kb on + strand, within BT2214 at 2782.814 kb on - strand, within BT2214 at 2782.826 kb on + strand, within BT2214 at 2782.827 kb on - strand, within BT2214 at 2782.827 kb on - strand, within BT2214 at 2782.827 kb on - strand, within BT2214 at 2782.830 kb on - strand, within BT2214 at 2782.832 kb on + strand, within BT2214 at 2782.833 kb on - strand, within BT2214 at 2782.934 kb on + strand, within BT2214 at 2782.950 kb on + strand, within BT2214 at 2783.031 kb on + strand at 2783.041 kb on + strand at 2783.045 kb on + strand at 2783.045 kb on + strand at 2783.045 kb on + strand at 2783.051 kb on + strand at 2783.067 kb on + strand at 2783.089 kb on + strand at 2783.094 kb on + strand at 2783.098 kb on + strand at 2783.099 kb on - strand at 2783.179 kb on + strand, within BT2215 at 2783.180 kb on - strand, within BT2215 at 2783.185 kb on - strand, within BT2215 at 2783.209 kb on - strand, within BT2215 at 2783.211 kb on - strand, within BT2215 at 2783.249 kb on - strand, within BT2215 at 2783.261 kb on + strand, within BT2215 at 2783.283 kb on - strand, within BT2215 at 2783.296 kb on - strand, within BT2215 at 2783.312 kb on - strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.316 kb on + strand, within BT2215 at 2783.317 kb on - strand, within BT2215 at 2783.322 kb on + strand, within BT2215 at 2783.323 kb on - strand, within BT2215 at 2783.323 kb on - strand, within BT2215 at 2783.323 kb on - strand, within BT2215 at 2783.409 kb on + strand, within BT2215
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM remove 2,780,379 - BT2212 0.23 +1.5 2,780,380 + BT2212 0.23 -3.3 2,780,424 - BT2212 0.27 -1.2 2,780,436 + BT2212 0.29 -0.6 2,780,436 + BT2212 0.29 +1.6 2,780,443 + BT2212 0.29 -3.0 2,780,524 + BT2212 0.38 -1.2 2,780,538 + BT2212 0.39 +0.4 2,780,538 + BT2212 0.39 -0.6 2,780,582 + BT2212 0.43 +1.2 2,780,676 + BT2212 0.53 -2.4 2,780,681 + BT2212 0.54 +0.2 2,780,682 - BT2212 0.54 +0.4 2,780,698 - BT2212 0.55 -3.9 2,780,719 + BT2212 0.58 -1.6 2,780,720 - BT2212 0.58 -2.2 2,780,795 - BT2212 0.65 +0.4 2,780,848 - BT2212 0.71 -0.6 2,780,987 - BT2212 0.85 +0.0 2,781,132 + -0.6 2,781,132 + -1.9 2,781,136 + -0.3 2,781,146 - -3.8 2,781,146 - -0.6 2,781,180 - -0.6 2,781,367 + BT2213 0.17 -0.5 2,781,438 - BT2213 0.23 +0.3 2,781,503 + BT2213 0.28 -0.6 2,781,503 + BT2213 0.28 -1.0 2,781,541 + BT2213 0.31 -0.9 2,781,542 - BT2213 0.31 +0.9 2,781,617 + BT2213 0.37 +1.1 2,781,617 + BT2213 0.37 -0.6 2,781,619 + -0.6 2,781,676 + BT2213 0.42 -0.1 2,781,787 + BT2213 0.50 +0.4 2,781,788 - BT2213 0.51 -3.7 2,781,788 - BT2213 0.51 -2.9 2,781,968 - BT2213 0.65 -2.7 2,782,034 - BT2213 0.70 -1.6 2,782,055 + BT2213 0.71 -1.3 2,782,055 + BT2213 0.71 +1.7 2,782,100 + BT2213 0.75 +1.6 2,782,100 + BT2213 0.75 -1.9 2,782,101 - BT2213 0.75 +1.0 2,782,101 - BT2213 0.75 +0.4 2,782,101 - BT2213 0.75 +1.2 2,782,134 + BT2213 0.78 -1.9 2,782,134 + BT2213 0.78 +1.4 2,782,135 - BT2213 0.78 -2.2 2,782,135 - BT2213 0.78 +0.4 2,782,184 + BT2213 0.82 -2.6 2,782,238 + BT2213 0.86 +0.3 2,782,239 - BT2213 0.86 +0.4 2,782,330 - -0.6 2,782,356 + -0.3 2,782,560 + BT2214 0.24 -0.2 2,782,576 + BT2214 0.26 -2.7 2,782,577 - BT2214 0.26 -3.7 2,782,578 + BT2214 0.27 -0.3 2,782,742 - BT2214 0.53 +1.8 2,782,763 + BT2214 0.57 -1.2 2,782,764 - BT2214 0.57 -1.1 2,782,764 - BT2214 0.57 +0.4 2,782,781 - -1.6 2,782,813 + BT2214 0.65 -1.3 2,782,814 - BT2214 0.65 -1.2 2,782,826 + BT2214 0.67 -0.6 2,782,827 - BT2214 0.67 -0.1 2,782,827 - BT2214 0.67 +0.4 2,782,827 - BT2214 0.67 -1.2 2,782,830 - BT2214 0.67 -0.6 2,782,832 + BT2214 0.68 -1.0 2,782,833 - BT2214 0.68 -2.2 2,782,934 + BT2214 0.84 -1.9 2,782,950 + BT2214 0.87 +1.0 2,783,031 + -1.9 2,783,041 + -0.6 2,783,045 + +1.4 2,783,045 + -0.5 2,783,045 + -3.3 2,783,051 + +0.4 2,783,067 + -3.5 2,783,089 + +0.4 2,783,094 + -1.5 2,783,098 + -1.2 2,783,099 - +0.4 2,783,179 + BT2215 0.14 +1.0 2,783,180 - BT2215 0.14 -1.5 2,783,185 - BT2215 0.14 -0.8 2,783,209 - BT2215 0.17 -1.9 2,783,211 - BT2215 0.17 -1.2 2,783,249 - BT2215 0.21 -0.6 2,783,261 + BT2215 0.23 +0.4 2,783,283 - BT2215 0.25 -1.6 2,783,296 - BT2215 0.26 -0.6 2,783,312 - BT2215 0.28 +0.4 2,783,316 + BT2215 0.29 -2.2 2,783,316 + BT2215 0.29 -2.0 2,783,316 + BT2215 0.29 +0.9 2,783,316 + BT2215 0.29 -2.4 2,783,316 + BT2215 0.29 -2.9 2,783,317 - BT2215 0.29 -3.0 2,783,322 + BT2215 0.29 +0.4 2,783,323 - BT2215 0.29 -2.8 2,783,323 - BT2215 0.29 -2.6 2,783,323 - BT2215 0.29 +1.3 2,783,409 + BT2215 0.38 -1.6
Or see this region's nucleotide sequence