Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1007 and BT1008 are separated by 66 nucleotides BT1008 and BT1009 are separated by 5 nucleotides
BT1007: BT1007 - conserved hypothetical protein (NCBI ptt file), at 1,254,189 to 1,254,980
BT1007
BT1008: BT1008 - xylanase (NCBI ptt file), at 1,255,047 to 1,255,835
BT1008
BT1009: BT1009 - dihydroorotate dehydrogenase (NCBI ptt file), at 1,255,841 to 1,256,779
BT1009
Position (kb)
1255
1256 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1254.060 kb on + strand at 1254.078 kb on - strand at 1254.092 kb on + strand at 1254.152 kb on + strand at 1254.191 kb on - strand at 1254.228 kb on + strand at 1254.248 kb on + strand at 1254.257 kb on + strand at 1254.302 kb on - strand, within BT1007 at 1254.306 kb on + strand, within BT1007 at 1254.307 kb on - strand, within BT1007 at 1254.310 kb on - strand, within BT1007 at 1254.332 kb on + strand, within BT1007 at 1254.345 kb on - strand, within BT1007 at 1254.368 kb on + strand, within BT1007 at 1254.369 kb on - strand, within BT1007 at 1254.420 kb on - strand, within BT1007 at 1254.424 kb on + strand, within BT1007 at 1254.427 kb on - strand, within BT1007 at 1254.516 kb on + strand, within BT1007 at 1254.517 kb on - strand, within BT1007 at 1254.525 kb on - strand, within BT1007 at 1254.607 kb on + strand, within BT1007 at 1254.609 kb on + strand, within BT1007 at 1254.630 kb on - strand, within BT1007 at 1254.630 kb on - strand, within BT1007 at 1254.634 kb on - strand, within BT1007 at 1254.778 kb on - strand, within BT1007 at 1254.780 kb on - strand, within BT1007 at 1254.803 kb on + strand, within BT1007 at 1254.919 kb on - strand at 1254.956 kb on + strand at 1254.956 kb on + strand at 1255.013 kb on + strand at 1255.013 kb on + strand at 1255.022 kb on + strand at 1255.097 kb on - strand at 1255.164 kb on - strand, within BT1008 at 1255.193 kb on + strand, within BT1008 at 1255.194 kb on - strand, within BT1008 at 1255.237 kb on - strand, within BT1008 at 1255.268 kb on + strand, within BT1008 at 1255.285 kb on + strand, within BT1008 at 1255.309 kb on + strand, within BT1008 at 1255.585 kb on + strand, within BT1008 at 1255.586 kb on - strand, within BT1008 at 1255.586 kb on - strand, within BT1008 at 1255.589 kb on + strand, within BT1008 at 1255.590 kb on - strand, within BT1008 at 1255.674 kb on - strand, within BT1008 at 1255.783 kb on + strand at 1255.784 kb on - strand at 1255.807 kb on + strand at 1255.815 kb on - strand at 1255.818 kb on - strand at 1255.895 kb on - strand at 1256.016 kb on + strand, within BT1009 at 1256.016 kb on + strand, within BT1009 at 1256.333 kb on + strand, within BT1009 at 1256.334 kb on - strand, within BT1009 at 1256.468 kb on + strand, within BT1009 at 1256.562 kb on + strand, within BT1009 at 1256.754 kb on + strand at 1256.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM remove 1,254,060 + -1.0 1,254,078 - -0.5 1,254,092 + -0.9 1,254,152 + -2.3 1,254,191 - -1.1 1,254,228 + +0.7 1,254,248 + -2.3 1,254,257 + -1.1 1,254,302 - BT1007 0.14 +0.0 1,254,306 + BT1007 0.15 -1.5 1,254,307 - BT1007 0.15 -0.5 1,254,310 - BT1007 0.15 -2.7 1,254,332 + BT1007 0.18 -0.5 1,254,345 - BT1007 0.20 +0.5 1,254,368 + BT1007 0.23 -1.1 1,254,369 - BT1007 0.23 -1.9 1,254,420 - BT1007 0.29 +0.5 1,254,424 + BT1007 0.30 -0.5 1,254,427 - BT1007 0.30 -2.8 1,254,516 + BT1007 0.41 -1.8 1,254,517 - BT1007 0.41 -1.1 1,254,525 - BT1007 0.42 -2.1 1,254,607 + BT1007 0.53 -3.1 1,254,609 + BT1007 0.53 -1.8 1,254,630 - BT1007 0.56 -3.4 1,254,630 - BT1007 0.56 +0.0 1,254,634 - BT1007 0.56 +0.5 1,254,778 - BT1007 0.74 +0.5 1,254,780 - BT1007 0.75 +3.6 1,254,803 + BT1007 0.78 -2.1 1,254,919 - -1.5 1,254,956 + +0.5 1,254,956 + +0.5 1,255,013 + +2.3 1,255,013 + -1.2 1,255,022 + +0.5 1,255,097 - -0.5 1,255,164 - BT1008 0.15 -2.2 1,255,193 + BT1008 0.19 -1.1 1,255,194 - BT1008 0.19 -0.5 1,255,237 - BT1008 0.24 -4.9 1,255,268 + BT1008 0.28 -3.0 1,255,285 + BT1008 0.30 -0.5 1,255,309 + BT1008 0.33 +0.5 1,255,585 + BT1008 0.68 +0.5 1,255,586 - BT1008 0.68 -0.5 1,255,586 - BT1008 0.68 -0.5 1,255,589 + BT1008 0.69 -1.8 1,255,590 - BT1008 0.69 +0.5 1,255,674 - BT1008 0.79 -0.5 1,255,783 + -1.1 1,255,784 - -1.8 1,255,807 + +0.5 1,255,815 - -2.3 1,255,818 - -1.1 1,255,895 - -0.5 1,256,016 + BT1009 0.19 +1.0 1,256,016 + BT1009 0.19 +3.2 1,256,333 + BT1009 0.52 +0.5 1,256,334 - BT1009 0.53 -1.5 1,256,468 + BT1009 0.67 -1.5 1,256,562 + BT1009 0.77 -2.7 1,256,754 + -2.5 1,256,787 - -0.1
Or see this region's nucleotide sequence