Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0992 and BT0993 are separated by 51 nucleotides BT0993 and BT0994 are separated by 355 nucleotides
BT0992: BT0992 - beta-galactosidase (NCBI ptt file), at 1,222,940 to 1,225,792
BT0992
BT0993: BT0993 - beta-galactosidase (NCBI ptt file), at 1,225,844 to 1,229,122
BT0993
BT0994: BT0994 - two-component system histidine kinase (NCBI ptt file), at 1,229,478 to 1,230,977
BT0994
Position (kb)
1225
1226
1227
1228
1229
1230 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1225.263 kb on + strand, within BT0992 at 1225.514 kb on - strand at 1225.516 kb on - strand at 1225.581 kb on + strand at 1225.581 kb on + strand at 1225.676 kb on + strand at 1225.676 kb on + strand at 1225.676 kb on + strand at 1225.677 kb on - strand at 1225.681 kb on + strand at 1225.814 kb on - strand at 1225.824 kb on + strand at 1225.863 kb on - strand at 1225.873 kb on + strand at 1225.908 kb on - strand at 1225.927 kb on + strand at 1225.957 kb on + strand at 1226.012 kb on + strand at 1226.027 kb on - strand at 1226.151 kb on + strand at 1226.156 kb on + strand at 1226.156 kb on + strand at 1226.157 kb on - strand at 1226.157 kb on - strand at 1226.252 kb on - strand, within BT0993 at 1226.258 kb on - strand, within BT0993 at 1226.450 kb on + strand, within BT0993 at 1226.789 kb on - strand, within BT0993 at 1226.866 kb on + strand, within BT0993 at 1226.937 kb on - strand, within BT0993 at 1226.941 kb on + strand, within BT0993 at 1227.045 kb on - strand, within BT0993 at 1227.051 kb on - strand, within BT0993 at 1227.232 kb on + strand, within BT0993 at 1227.338 kb on - strand, within BT0993 at 1227.365 kb on - strand, within BT0993 at 1227.493 kb on - strand, within BT0993 at 1227.579 kb on + strand, within BT0993 at 1227.808 kb on - strand, within BT0993 at 1227.902 kb on + strand, within BT0993 at 1227.902 kb on + strand, within BT0993 at 1227.903 kb on - strand, within BT0993 at 1228.025 kb on + strand, within BT0993 at 1228.026 kb on - strand, within BT0993 at 1228.062 kb on - strand, within BT0993 at 1228.098 kb on - strand, within BT0993 at 1228.259 kb on + strand, within BT0993 at 1228.303 kb on + strand, within BT0993 at 1228.304 kb on - strand, within BT0993 at 1228.331 kb on + strand, within BT0993 at 1228.336 kb on + strand, within BT0993 at 1228.336 kb on + strand, within BT0993 at 1228.405 kb on + strand, within BT0993 at 1228.432 kb on + strand, within BT0993 at 1228.450 kb on + strand, within BT0993 at 1228.516 kb on - strand, within BT0993 at 1228.529 kb on + strand, within BT0993 at 1228.588 kb on + strand, within BT0993 at 1228.597 kb on + strand, within BT0993 at 1228.740 kb on + strand at 1228.815 kb on + strand at 1228.880 kb on - strand at 1229.106 kb on - strand at 1229.110 kb on + strand at 1229.110 kb on + strand at 1229.191 kb on + strand at 1229.255 kb on - strand at 1229.280 kb on + strand at 1229.399 kb on - strand at 1229.432 kb on + strand at 1229.444 kb on + strand at 1229.528 kb on - strand at 1229.569 kb on + strand at 1229.667 kb on - strand, within BT0994 at 1229.669 kb on + strand, within BT0994 at 1229.769 kb on - strand, within BT0994 at 1229.875 kb on - strand, within BT0994 at 1229.961 kb on + strand, within BT0994
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM remove 1,225,263 + BT0992 0.81 -0.2 1,225,514 - -0.5 1,225,516 - -0.5 1,225,581 + -3.0 1,225,581 + -1.5 1,225,676 + +0.5 1,225,676 + -2.7 1,225,676 + -1.5 1,225,677 - -1.8 1,225,681 + -0.5 1,225,814 - +1.3 1,225,824 + +0.5 1,225,863 - +0.5 1,225,873 + -1.5 1,225,908 - -1.5 1,225,927 + +2.8 1,225,957 + -2.1 1,226,012 + -0.5 1,226,027 - -1.5 1,226,151 + -1.5 1,226,156 + -0.5 1,226,156 + -0.5 1,226,157 - +0.5 1,226,157 - -2.1 1,226,252 - BT0993 0.12 +0.5 1,226,258 - BT0993 0.13 -1.1 1,226,450 + BT0993 0.18 -0.5 1,226,789 - BT0993 0.29 -1.5 1,226,866 + BT0993 0.31 +1.2 1,226,937 - BT0993 0.33 -0.5 1,226,941 + BT0993 0.33 +0.5 1,227,045 - BT0993 0.37 -1.2 1,227,051 - BT0993 0.37 -2.3 1,227,232 + BT0993 0.42 -1.1 1,227,338 - BT0993 0.46 +0.5 1,227,365 - BT0993 0.46 -0.5 1,227,493 - BT0993 0.50 +0.5 1,227,579 + BT0993 0.53 -2.1 1,227,808 - BT0993 0.60 +1.2 1,227,902 + BT0993 0.63 -3.0 1,227,902 + BT0993 0.63 -3.8 1,227,903 - BT0993 0.63 +0.5 1,228,025 + BT0993 0.67 -1.1 1,228,026 - BT0993 0.67 -0.5 1,228,062 - BT0993 0.68 +0.5 1,228,098 - BT0993 0.69 -2.1 1,228,259 + BT0993 0.74 -0.5 1,228,303 + BT0993 0.75 +0.5 1,228,304 - BT0993 0.75 -0.5 1,228,331 + BT0993 0.76 +0.5 1,228,336 + BT0993 0.76 -2.3 1,228,336 + BT0993 0.76 -1.8 1,228,405 + BT0993 0.78 -0.5 1,228,432 + BT0993 0.79 -1.5 1,228,450 + BT0993 0.79 -1.0 1,228,516 - BT0993 0.81 -0.7 1,228,529 + BT0993 0.82 -2.5 1,228,588 + BT0993 0.84 +0.5 1,228,597 + BT0993 0.84 +0.5 1,228,740 + -1.5 1,228,815 + -1.5 1,228,880 - -2.7 1,229,106 - +3.3 1,229,110 + -0.4 1,229,110 + -1.5 1,229,191 + -0.5 1,229,255 - -2.5 1,229,280 + -3.0 1,229,399 - -1.5 1,229,432 + -3.5 1,229,444 + +0.5 1,229,528 - -2.3 1,229,569 + -0.5 1,229,667 - BT0994 0.13 -1.7 1,229,669 + BT0994 0.13 -2.3 1,229,769 - BT0994 0.19 +0.5 1,229,875 - BT0994 0.26 -0.5 1,229,961 + BT0994 0.32 -1.5
Or see this region's nucleotide sequence