Experiment: BHIS with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0958 and BT0959 are separated by 59 nucleotides BT0959 and BT0960 are separated by 12 nucleotides BT0960 and BT0961 are separated by 73 nucleotides BT0961 and BT0962 overlap by 1 nucleotides
BT0958: BT0958 - two-component system sensor histidine kinase/response regulator, hybrid ('one component system') (NCBI ptt file), at 1,169,220 to 1,173,209
BT0958
BT0959: BT0959 - conserved hypothetical protein (NCBI ptt file), at 1,173,269 to 1,173,778
BT0959
BT0960: BT0960 - putative TonB-dependent outer membrane receptor (NCBI ptt file), at 1,173,791 to 1,175,632
BT0960
BT0961: BT0961 - conserved hypothetical protein (NCBI ptt file), at 1,175,706 to 1,176,140
BT0961
BT0962: BT0962 - aldo/keto reductase (NCBI ptt file), at 1,176,140 to 1,177,543
BT0962
Position (kb)
1173
1174
1175
1176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1172.800 kb on + strand, within BT0958 at 1172.804 kb on + strand, within BT0958 at 1172.804 kb on + strand, within BT0958 at 1172.804 kb on + strand, within BT0958 at 1172.805 kb on - strand, within BT0958 at 1172.805 kb on - strand, within BT0958 at 1172.807 kb on - strand, within BT0958 at 1172.834 kb on + strand at 1172.841 kb on - strand at 1172.897 kb on + strand at 1172.939 kb on + strand at 1172.959 kb on - strand at 1172.977 kb on + strand at 1172.986 kb on + strand at 1172.987 kb on - strand at 1172.987 kb on - strand at 1173.004 kb on + strand at 1173.057 kb on - strand at 1173.131 kb on + strand at 1173.164 kb on + strand at 1173.215 kb on + strand at 1173.378 kb on + strand, within BT0959 at 1173.379 kb on - strand, within BT0959 at 1173.379 kb on - strand, within BT0959 at 1173.379 kb on - strand, within BT0959 at 1173.435 kb on - strand, within BT0959 at 1173.489 kb on - strand, within BT0959 at 1173.490 kb on - strand, within BT0959 at 1173.538 kb on + strand, within BT0959 at 1173.609 kb on + strand, within BT0959 at 1173.660 kb on - strand, within BT0959 at 1173.740 kb on - strand at 1173.785 kb on - strand at 1174.008 kb on + strand, within BT0960 at 1174.062 kb on + strand, within BT0960 at 1174.114 kb on + strand, within BT0960 at 1174.116 kb on + strand, within BT0960 at 1174.232 kb on - strand, within BT0960 at 1174.252 kb on + strand, within BT0960 at 1174.265 kb on - strand, within BT0960 at 1174.366 kb on + strand, within BT0960 at 1174.384 kb on + strand, within BT0960 at 1174.532 kb on + strand, within BT0960 at 1174.833 kb on + strand, within BT0960 at 1174.861 kb on + strand, within BT0960 at 1174.904 kb on + strand, within BT0960 at 1174.944 kb on - strand, within BT0960 at 1175.005 kb on - strand, within BT0960 at 1175.041 kb on + strand, within BT0960 at 1175.041 kb on + strand, within BT0960 at 1175.065 kb on + strand, within BT0960 at 1175.121 kb on + strand, within BT0960 at 1175.183 kb on + strand, within BT0960 at 1175.192 kb on + strand, within BT0960 at 1175.218 kb on - strand, within BT0960 at 1175.274 kb on + strand, within BT0960 at 1175.416 kb on + strand, within BT0960 at 1175.419 kb on + strand, within BT0960 at 1175.510 kb on + strand at 1175.521 kb on + strand at 1175.843 kb on + strand, within BT0961 at 1175.924 kb on + strand, within BT0961 at 1175.979 kb on + strand, within BT0961 at 1176.069 kb on - strand, within BT0961 at 1176.072 kb on + strand, within BT0961 at 1176.072 kb on + strand, within BT0961 at 1176.073 kb on - strand, within BT0961 at 1176.073 kb on - strand, within BT0961 at 1176.117 kb on + strand at 1176.141 kb on + strand at 1176.200 kb on + strand at 1176.203 kb on - strand at 1176.206 kb on + strand at 1176.375 kb on + strand, within BT0962 at 1176.456 kb on + strand, within BT0962 at 1176.529 kb on - strand, within BT0962 at 1176.560 kb on - strand, within BT0962 at 1176.561 kb on + strand, within BT0962 at 1176.587 kb on + strand, within BT0962 at 1176.587 kb on + strand, within BT0962 at 1176.630 kb on - strand, within BT0962
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.0001953125 mM remove 1,172,800 + BT0958 0.90 -3.2 1,172,804 + BT0958 0.90 -0.5 1,172,804 + BT0958 0.90 -1.1 1,172,804 + BT0958 0.90 +1.3 1,172,805 - BT0958 0.90 +1.1 1,172,805 - BT0958 0.90 +0.5 1,172,807 - BT0958 0.90 -1.8 1,172,834 + +0.5 1,172,841 - -1.1 1,172,897 + +0.5 1,172,939 + -0.5 1,172,959 - +1.3 1,172,977 + -0.5 1,172,986 + -3.4 1,172,987 - +0.5 1,172,987 - -1.5 1,173,004 + +3.0 1,173,057 - -0.9 1,173,131 + +0.5 1,173,164 + +0.8 1,173,215 + -3.5 1,173,378 + BT0959 0.21 -1.1 1,173,379 - BT0959 0.22 -1.1 1,173,379 - BT0959 0.22 -2.5 1,173,379 - BT0959 0.22 -2.3 1,173,435 - BT0959 0.33 -1.1 1,173,489 - BT0959 0.43 -3.7 1,173,490 - BT0959 0.43 +0.7 1,173,538 + BT0959 0.53 +0.5 1,173,609 + BT0959 0.67 -2.7 1,173,660 - BT0959 0.77 +1.7 1,173,740 - -0.5 1,173,785 - -0.5 1,174,008 + BT0960 0.12 -3.3 1,174,062 + BT0960 0.15 +0.7 1,174,114 + BT0960 0.18 -1.1 1,174,116 + BT0960 0.18 -1.1 1,174,232 - BT0960 0.24 +2.7 1,174,252 + BT0960 0.25 -1.7 1,174,265 - BT0960 0.26 -0.5 1,174,366 + BT0960 0.31 +0.5 1,174,384 + BT0960 0.32 +2.1 1,174,532 + BT0960 0.40 -0.5 1,174,833 + BT0960 0.57 -0.5 1,174,861 + BT0960 0.58 +0.9 1,174,904 + BT0960 0.60 -3.6 1,174,944 - BT0960 0.63 -1.1 1,175,005 - BT0960 0.66 -3.6 1,175,041 + BT0960 0.68 +0.5 1,175,041 + BT0960 0.68 -2.8 1,175,065 + BT0960 0.69 -1.5 1,175,121 + BT0960 0.72 +1.8 1,175,183 + BT0960 0.76 +0.5 1,175,192 + BT0960 0.76 +3.3 1,175,218 - BT0960 0.77 +0.5 1,175,274 + BT0960 0.81 -0.8 1,175,416 + BT0960 0.88 -1.1 1,175,419 + BT0960 0.88 +1.1 1,175,510 + -0.5 1,175,521 + +1.4 1,175,843 + BT0961 0.31 -0.9 1,175,924 + BT0961 0.50 -1.1 1,175,979 + BT0961 0.63 -1.8 1,176,069 - BT0961 0.83 -2.3 1,176,072 + BT0961 0.84 +0.5 1,176,072 + BT0961 0.84 -0.5 1,176,073 - BT0961 0.84 -1.1 1,176,073 - BT0961 0.84 +0.9 1,176,117 + -1.1 1,176,141 + +0.5 1,176,200 + -1.8 1,176,203 - -1.1 1,176,206 + -0.3 1,176,375 + BT0962 0.17 -0.5 1,176,456 + BT0962 0.23 -3.2 1,176,529 - BT0962 0.28 -2.3 1,176,560 - BT0962 0.30 -0.5 1,176,561 + BT0962 0.30 +0.5 1,176,587 + BT0962 0.32 -1.1 1,176,587 + BT0962 0.32 +0.5 1,176,630 - BT0962 0.35 +3.7
Or see this region's nucleotide sequence