Experiment: BG11 with Chlorate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psaJ and psaF are separated by 25 nucleotides psaF and Synpcc7942_1251 are separated by 177 nucleotides Synpcc7942_1251 and Synpcc7942_1252 are separated by 68 nucleotides
Synpcc7942_1249: psaJ - photosystem I reaction center subunit IX, at 1,273,621 to 1,273,755
psaJ
Synpcc7942_1250: psaF - photosystem I reaction center subunit III precursor, at 1,273,781 to 1,274,260
psaF
Synpcc7942_1251: Synpcc7942_1251 - O-sialoglycoprotein endopeptidase, at 1,274,438 to 1,275,487
_1251
Synpcc7942_1252: Synpcc7942_1252 - DNA repair protein RAD32-like, at 1,275,556 to 1,276,833
_1252
Position (kb)
1274
1275
1276 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1273.469 kb on + strand at 1273.587 kb on - strand at 1273.962 kb on - strand, within psaF at 1274.169 kb on - strand, within psaF at 1274.193 kb on + strand, within psaF at 1274.218 kb on + strand at 1274.271 kb on + strand at 1274.285 kb on + strand at 1274.285 kb on + strand at 1274.293 kb on - strand at 1275.466 kb on + strand at 1275.482 kb on + strand at 1275.488 kb on - strand at 1275.490 kb on + strand at 1275.492 kb on + strand at 1275.499 kb on - strand at 1275.500 kb on - strand at 1275.500 kb on - strand at 1275.500 kb on - strand at 1275.505 kb on - strand at 1275.507 kb on + strand at 1275.524 kb on + strand at 1275.530 kb on - strand at 1275.643 kb on + strand at 1275.683 kb on - strand at 1275.760 kb on + strand, within Synpcc7942_1252 at 1275.761 kb on + strand, within Synpcc7942_1252 at 1275.762 kb on + strand, within Synpcc7942_1252 at 1275.768 kb on - strand, within Synpcc7942_1252 at 1275.781 kb on + strand, within Synpcc7942_1252 at 1275.789 kb on - strand, within Synpcc7942_1252 at 1275.833 kb on - strand, within Synpcc7942_1252 at 1275.833 kb on - strand, within Synpcc7942_1252 at 1275.833 kb on - strand, within Synpcc7942_1252 at 1275.852 kb on + strand, within Synpcc7942_1252 at 1275.929 kb on - strand, within Synpcc7942_1252 at 1275.970 kb on - strand, within Synpcc7942_1252 at 1275.980 kb on - strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.070 kb on + strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.078 kb on - strand, within Synpcc7942_1252 at 1276.124 kb on + strand, within Synpcc7942_1252 at 1276.135 kb on + strand, within Synpcc7942_1252 at 1276.158 kb on - strand, within Synpcc7942_1252 at 1276.169 kb on + strand, within Synpcc7942_1252 at 1276.179 kb on + strand, within Synpcc7942_1252 at 1276.204 kb on - strand, within Synpcc7942_1252 at 1276.273 kb on + strand, within Synpcc7942_1252 at 1276.281 kb on - strand, within Synpcc7942_1252 at 1276.295 kb on - strand, within Synpcc7942_1252 at 1276.299 kb on + strand, within Synpcc7942_1252 at 1276.311 kb on + strand, within Synpcc7942_1252 at 1276.321 kb on + strand, within Synpcc7942_1252 at 1276.321 kb on + strand, within Synpcc7942_1252 at 1276.321 kb on + strand, within Synpcc7942_1252 at 1276.329 kb on - strand, within Synpcc7942_1252 at 1276.367 kb on + strand, within Synpcc7942_1252 at 1276.484 kb on + strand, within Synpcc7942_1252
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chlorate 5 mM remove 1,273,469 + -2.1 1,273,587 - -0.6 1,273,962 - psaF Synpcc7942_1250 0.38 -1.4 1,274,169 - psaF Synpcc7942_1250 0.81 -0.8 1,274,193 + psaF Synpcc7942_1250 0.86 -1.6 1,274,218 + -0.8 1,274,271 + -1.2 1,274,285 + -0.6 1,274,285 + +1.8 1,274,293 - -1.1 1,275,466 + +1.8 1,275,482 + -0.4 1,275,488 - -1.5 1,275,490 + -0.9 1,275,492 + +0.9 1,275,499 - -0.6 1,275,500 - -0.6 1,275,500 - -0.8 1,275,500 - +1.2 1,275,505 - -0.0 1,275,507 + -0.5 1,275,524 + +0.4 1,275,530 - +1.0 1,275,643 + -0.8 1,275,683 - -1.3 1,275,760 + Synpcc7942_1252 0.16 -1.1 1,275,761 + Synpcc7942_1252 0.16 -1.2 1,275,762 + Synpcc7942_1252 0.16 -0.6 1,275,768 - Synpcc7942_1252 0.17 -1.8 1,275,781 + Synpcc7942_1252 0.18 -1.4 1,275,789 - Synpcc7942_1252 0.18 -0.1 1,275,833 - Synpcc7942_1252 0.22 -1.8 1,275,833 - Synpcc7942_1252 0.22 -1.9 1,275,833 - Synpcc7942_1252 0.22 -0.8 1,275,852 + Synpcc7942_1252 0.23 -2.3 1,275,929 - Synpcc7942_1252 0.29 -2.2 1,275,970 - Synpcc7942_1252 0.32 -1.4 1,275,980 - Synpcc7942_1252 0.33 -0.6 1,276,070 + Synpcc7942_1252 0.40 -1.9 1,276,070 + Synpcc7942_1252 0.40 -1.1 1,276,070 + Synpcc7942_1252 0.40 -0.6 1,276,070 + Synpcc7942_1252 0.40 -1.5 1,276,070 + Synpcc7942_1252 0.40 -0.6 1,276,070 + Synpcc7942_1252 0.40 -0.8 1,276,078 - Synpcc7942_1252 0.41 -2.5 1,276,078 - Synpcc7942_1252 0.41 -3.2 1,276,078 - Synpcc7942_1252 0.41 -2.1 1,276,078 - Synpcc7942_1252 0.41 -1.1 1,276,078 - Synpcc7942_1252 0.41 -0.5 1,276,078 - Synpcc7942_1252 0.41 -1.2 1,276,124 + Synpcc7942_1252 0.44 -1.6 1,276,135 + Synpcc7942_1252 0.45 -1.0 1,276,158 - Synpcc7942_1252 0.47 -1.7 1,276,169 + Synpcc7942_1252 0.48 +1.2 1,276,179 + Synpcc7942_1252 0.49 -3.5 1,276,204 - Synpcc7942_1252 0.51 -0.6 1,276,273 + Synpcc7942_1252 0.56 -1.9 1,276,281 - Synpcc7942_1252 0.57 -4.2 1,276,295 - Synpcc7942_1252 0.58 -1.5 1,276,299 + Synpcc7942_1252 0.58 -1.2 1,276,311 + Synpcc7942_1252 0.59 -2.1 1,276,321 + Synpcc7942_1252 0.60 -2.0 1,276,321 + Synpcc7942_1252 0.60 -0.1 1,276,321 + Synpcc7942_1252 0.60 -1.4 1,276,329 - Synpcc7942_1252 0.60 -1.5 1,276,367 + Synpcc7942_1252 0.63 -3.6 1,276,484 + Synpcc7942_1252 0.73 -0.6
Or see this region's nucleotide sequence