Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_99

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_98 and Pf1N1B4_99 are separated by 24 nucleotidesPf1N1B4_99 and Pf1N1B4_100 are separated by 308 nucleotidesPf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotides Pf1N1B4_98 - Nucleoside-specific channel-forming protein Tsx precursor, at 103,531 to 104,487 _98 Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546 _99 Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082 _100 Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311 _101 Position (kb) 104 105 106Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 103.529 kb on + strandat 103.705 kb on + strand, within Pf1N1B4_98at 103.789 kb on + strand, within Pf1N1B4_98at 103.798 kb on + strand, within Pf1N1B4_98at 103.798 kb on + strand, within Pf1N1B4_98at 103.799 kb on - strand, within Pf1N1B4_98at 103.804 kb on + strand, within Pf1N1B4_98at 103.804 kb on + strand, within Pf1N1B4_98at 103.804 kb on + strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strand, within Pf1N1B4_98at 103.805 kb on - strandat 103.834 kb on + strand, within Pf1N1B4_98at 103.993 kb on + strand, within Pf1N1B4_98at 103.993 kb on + strand, within Pf1N1B4_98at 103.993 kb on + strandat 103.993 kb on + strand, within Pf1N1B4_98at 103.994 kb on - strand, within Pf1N1B4_98at 103.994 kb on - strand, within Pf1N1B4_98at 104.014 kb on + strand, within Pf1N1B4_98at 104.014 kb on + strand, within Pf1N1B4_98at 104.015 kb on - strand, within Pf1N1B4_98at 104.015 kb on - strand, within Pf1N1B4_98at 104.056 kb on + strand, within Pf1N1B4_98at 104.056 kb on + strandat 104.056 kb on + strand, within Pf1N1B4_98at 104.057 kb on - strand, within Pf1N1B4_98at 104.116 kb on + strand, within Pf1N1B4_98at 104.159 kb on - strand, within Pf1N1B4_98at 104.191 kb on + strand, within Pf1N1B4_98at 104.191 kb on + strand, within Pf1N1B4_98at 104.191 kb on + strandat 104.192 kb on - strand, within Pf1N1B4_98at 104.192 kb on - strand, within Pf1N1B4_98at 104.192 kb on - strand, within Pf1N1B4_98at 104.192 kb on - strand, within Pf1N1B4_98at 104.192 kb on - strand, within Pf1N1B4_98at 104.236 kb on + strand, within Pf1N1B4_98at 104.236 kb on + strand, within Pf1N1B4_98at 104.236 kb on + strand, within Pf1N1B4_98at 104.236 kb on + strand, within Pf1N1B4_98at 104.237 kb on - strand, within Pf1N1B4_98at 104.332 kb on + strand, within Pf1N1B4_98at 104.461 kb on + strandat 104.485 kb on + strandat 104.485 kb on + strandat 104.512 kb on - strandat 104.715 kb on + strand, within Pf1N1B4_99at 104.830 kb on + strand, within Pf1N1B4_99at 104.902 kb on + strand, within Pf1N1B4_99at 104.902 kb on + strand, within Pf1N1B4_99at 104.903 kb on - strand, within Pf1N1B4_99at 104.903 kb on - strandat 105.020 kb on - strand, within Pf1N1B4_99at 105.192 kb on + strand, within Pf1N1B4_99at 105.192 kb on + strand, within Pf1N1B4_99at 105.255 kb on + strand, within Pf1N1B4_99at 105.268 kb on + strand, within Pf1N1B4_99at 105.268 kb on + strand, within Pf1N1B4_99at 105.269 kb on - strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.302 kb on - strandat 105.302 kb on - strand, within Pf1N1B4_99at 105.428 kb on - strandat 105.657 kb on + strandat 105.657 kb on + strandat 105.657 kb on + strandat 105.658 kb on - strandat 105.791 kb on - strandat 105.828 kb on - strandat 105.828 kb on - strandat 105.829 kb on + strandat 105.830 kb on - strandat 105.830 kb on - strandat 105.833 kb on + strandat 105.833 kb on + strandat 105.833 kb on + strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.845 kb on + strandat 105.846 kb on - strandat 105.854 kb on + strandat 105.885 kb on + strand, within Pf1N1B4_100at 105.885 kb on + strand, within Pf1N1B4_100at 105.885 kb on + strand, within Pf1N1B4_100at 105.886 kb on - strand, within Pf1N1B4_100at 106.226 kb on + strand, within Pf1N1B4_101at 106.226 kb on + strand, within Pf1N1B4_101at 106.227 kb on - strand, within Pf1N1B4_101at 106.293 kb on + strand, within Pf1N1B4_101at 106.424 kb on + strandat 106.424 kb on + strandat 106.424 kb on + strandat 106.424 kb on + strandat 106.428 kb on - strandat 106.428 kb on - strandat 106.501 kb on + strandat 106.502 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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103,529 + +0.1
103,705 + Pf1N1B4_98 0.18 +0.1
103,789 + Pf1N1B4_98 0.27 -1.3
103,798 + Pf1N1B4_98 0.28 +0.1
103,798 + Pf1N1B4_98 0.28 +0.1
103,799 - Pf1N1B4_98 0.28 +0.1
103,804 + Pf1N1B4_98 0.29 -2.5
103,804 + Pf1N1B4_98 0.29 -1.2
103,804 + Pf1N1B4_98 0.29 -1.3
103,805 - Pf1N1B4_98 0.29 +0.1
103,805 - Pf1N1B4_98 0.29 -3.1
103,805 - Pf1N1B4_98 0.29 -4.6
103,805 - Pf1N1B4_98 0.29 +0.1
103,805 - -3.2
103,834 + Pf1N1B4_98 0.32 -2.2
103,993 + Pf1N1B4_98 0.48 +0.1
103,993 + Pf1N1B4_98 0.48 -3.1
103,993 + -2.7
103,993 + Pf1N1B4_98 0.48 -2.3
103,994 - Pf1N1B4_98 0.48 -2.6
103,994 - Pf1N1B4_98 0.48 +0.1
104,014 + Pf1N1B4_98 0.50 -1.2
104,014 + Pf1N1B4_98 0.50 -1.2
104,015 - Pf1N1B4_98 0.51 -2.5
104,015 - Pf1N1B4_98 0.51 -1.5
104,056 + Pf1N1B4_98 0.55 -1.5
104,056 + -2.5
104,056 + Pf1N1B4_98 0.55 +0.1
104,057 - Pf1N1B4_98 0.55 -0.2
104,116 + Pf1N1B4_98 0.61 +0.1
104,159 - Pf1N1B4_98 0.66 +0.1
104,191 + Pf1N1B4_98 0.69 +0.1
104,191 + Pf1N1B4_98 0.69 -2.2
104,191 + +0.1
104,192 - Pf1N1B4_98 0.69 +0.1
104,192 - Pf1N1B4_98 0.69 +0.1
104,192 - Pf1N1B4_98 0.69 +0.1
104,192 - Pf1N1B4_98 0.69 +0.1
104,192 - Pf1N1B4_98 0.69 +0.1
104,236 + Pf1N1B4_98 0.74 +0.1
104,236 + Pf1N1B4_98 0.74 +0.1
104,236 + Pf1N1B4_98 0.74 +0.1
104,236 + Pf1N1B4_98 0.74 +0.1
104,237 - Pf1N1B4_98 0.74 +0.1
104,332 + Pf1N1B4_98 0.84 +0.1
104,461 + +0.1
104,485 + +0.1
104,485 + -2.3
104,512 - -2.3
104,715 + Pf1N1B4_99 0.20 +0.1
104,830 + Pf1N1B4_99 0.31 -1.8
104,902 + Pf1N1B4_99 0.38 +0.1
104,902 + Pf1N1B4_99 0.38 -2.5
104,903 - Pf1N1B4_99 0.38 -3.4
104,903 - +0.1
105,020 - Pf1N1B4_99 0.49 +0.1
105,192 + Pf1N1B4_99 0.66 -1.5
105,192 + Pf1N1B4_99 0.66 -2.3
105,255 + Pf1N1B4_99 0.72 +0.1
105,268 + Pf1N1B4_99 0.73 -2.4
105,268 + Pf1N1B4_99 0.73 +0.1
105,269 - Pf1N1B4_99 0.73 -1.2
105,301 + Pf1N1B4_99 0.76 +0.1
105,301 + Pf1N1B4_99 0.76 +0.1
105,301 + Pf1N1B4_99 0.76 +0.1
105,301 + Pf1N1B4_99 0.76 +0.1
105,301 + Pf1N1B4_99 0.76 -2.6
105,302 - +0.1
105,302 - Pf1N1B4_99 0.76 -1.9
105,428 - -2.3
105,657 + -2.8
105,657 + -3.6
105,657 + +0.1
105,658 - -3.2
105,791 - +0.1
105,828 - +0.1
105,828 - -1.3
105,829 + +0.1
105,830 - -4.0
105,830 - -3.9
105,833 + +0.1
105,833 + +0.1
105,833 + +0.1
105,834 - +0.1
105,834 - -1.6
105,834 - +0.1
105,834 - +0.1
105,834 - -1.8
105,834 - -2.3
105,834 - +0.1
105,834 - +0.1
105,845 + +0.1
105,846 - +0.1
105,854 + -2.8
105,885 + Pf1N1B4_100 0.13 -1.0
105,885 + Pf1N1B4_100 0.13 +0.1
105,885 + Pf1N1B4_100 0.13 +0.1
105,886 - Pf1N1B4_100 0.14 +0.1
106,226 + Pf1N1B4_101 0.48 -3.2
106,226 + Pf1N1B4_101 0.48 +0.1
106,227 - Pf1N1B4_101 0.48 -2.5
106,293 + Pf1N1B4_101 0.88 -2.9
106,424 + +0.1
106,424 + +0.1
106,424 + -3.7
106,424 + +0.1
106,428 - -2.2
106,428 - -3.0
106,501 + -2.0
106,502 - +0.1

Or see this region's nucleotide sequence