Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_6043

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_6041 and Pf1N1B4_6042 are separated by 194 nucleotidesPf1N1B4_6042 and Pf1N1B4_6043 are separated by 113 nucleotidesPf1N1B4_6043 and Pf1N1B4_6044 are separated by 230 nucleotidesPf1N1B4_6044 and Pf1N1B4_6045 are separated by 243 nucleotides Pf1N1B4_6041 - Carbon storage regulator, at 504,393 to 504,587 _6041 Pf1N1B4_6042 - Probable transmembrane protein, at 504,782 to 505,237 _6042 Pf1N1B4_6043 - membrane protein, putative, at 505,351 to 506,565 _6043 Pf1N1B4_6044 - hypothetical protein, at 506,796 to 507,065 _6044 Pf1N1B4_6045 - CigR (Putative inner membrane protein), at 507,309 to 507,545 _6045 Position (kb) 505 506 507Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 504.369 kb on + strandat 504.370 kb on - strandat 504.400 kb on + strandat 504.427 kb on + strand, within Pf1N1B4_6041at 504.428 kb on - strand, within Pf1N1B4_6041at 504.446 kb on + strand, within Pf1N1B4_6041at 504.447 kb on - strand, within Pf1N1B4_6041at 504.711 kb on - strandat 504.854 kb on - strand, within Pf1N1B4_6042at 504.872 kb on + strandat 504.873 kb on - strand, within Pf1N1B4_6042at 504.873 kb on - strand, within Pf1N1B4_6042at 504.873 kb on - strand, within Pf1N1B4_6042at 505.015 kb on - strand, within Pf1N1B4_6042at 505.015 kb on - strand, within Pf1N1B4_6042at 505.018 kb on - strand, within Pf1N1B4_6042at 505.018 kb on - strand, within Pf1N1B4_6042at 505.018 kb on - strand, within Pf1N1B4_6042at 505.152 kb on - strand, within Pf1N1B4_6042at 505.152 kb on - strand, within Pf1N1B4_6042at 505.244 kb on - strandat 505.244 kb on - strandat 505.244 kb on - strandat 505.244 kb on - strandat 505.244 kb on - strandat 505.244 kb on - strandat 505.251 kb on - strandat 505.273 kb on + strandat 505.273 kb on + strandat 505.274 kb on - strandat 505.274 kb on - strandat 505.274 kb on - strandat 505.274 kb on - strandat 505.278 kb on + strandat 505.281 kb on + strandat 505.281 kb on + strandat 505.282 kb on - strandat 505.282 kb on - strandat 505.282 kb on - strandat 505.282 kb on - strandat 505.282 kb on - strandat 505.282 kb on - strandat 505.297 kb on - strandat 505.297 kb on - strandat 505.297 kb on - strandat 505.297 kb on - strandat 505.363 kb on + strandat 505.364 kb on - strandat 505.364 kb on - strandat 505.378 kb on - strandat 505.378 kb on - strandat 505.378 kb on - strandat 505.378 kb on - strandat 505.580 kb on - strand, within Pf1N1B4_6043at 505.616 kb on - strand, within Pf1N1B4_6043at 505.637 kb on - strand, within Pf1N1B4_6043at 505.637 kb on - strand, within Pf1N1B4_6043at 505.714 kb on + strandat 505.729 kb on + strand, within Pf1N1B4_6043at 505.730 kb on - strand, within Pf1N1B4_6043at 505.730 kb on - strand, within Pf1N1B4_6043at 505.735 kb on + strand, within Pf1N1B4_6043at 505.736 kb on - strand, within Pf1N1B4_6043at 505.736 kb on - strandat 505.753 kb on + strand, within Pf1N1B4_6043at 506.083 kb on + strand, within Pf1N1B4_6043at 506.084 kb on - strand, within Pf1N1B4_6043at 506.084 kb on - strand, within Pf1N1B4_6043at 506.104 kb on + strand, within Pf1N1B4_6043at 506.104 kb on + strand, within Pf1N1B4_6043at 506.104 kb on + strand, within Pf1N1B4_6043at 506.105 kb on - strand, within Pf1N1B4_6043at 506.105 kb on - strand, within Pf1N1B4_6043at 506.186 kb on + strandat 506.206 kb on - strand, within Pf1N1B4_6043at 506.206 kb on - strand, within Pf1N1B4_6043at 506.206 kb on - strand, within Pf1N1B4_6043at 506.206 kb on - strand, within Pf1N1B4_6043at 506.309 kb on + strand, within Pf1N1B4_6043at 506.309 kb on + strand, within Pf1N1B4_6043at 506.309 kb on + strand, within Pf1N1B4_6043at 506.310 kb on - strandat 506.310 kb on - strand, within Pf1N1B4_6043at 506.310 kb on - strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.311 kb on + strand, within Pf1N1B4_6043at 506.312 kb on - strand, within Pf1N1B4_6043at 506.312 kb on - strand, within Pf1N1B4_6043at 506.312 kb on - strandat 506.312 kb on - strand, within Pf1N1B4_6043at 506.312 kb on - strand, within Pf1N1B4_6043at 506.312 kb on - strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.315 kb on + strand, within Pf1N1B4_6043at 506.316 kb on - strand, within Pf1N1B4_6043at 506.316 kb on - strand, within Pf1N1B4_6043at 506.316 kb on - strand, within Pf1N1B4_6043at 506.316 kb on - strand, within Pf1N1B4_6043at 506.316 kb on - strand, within Pf1N1B4_6043at 506.563 kb on + strandat 506.563 kb on + strandat 506.563 kb on + strandat 506.563 kb on + strandat 506.563 kb on + strandat 506.564 kb on - strandat 506.666 kb on + strandat 506.667 kb on - strandat 507.153 kb on + strandat 507.165 kb on + strandat 507.165 kb on + strandat 507.165 kb on + strandat 507.166 kb on - strandat 507.166 kb on - strandat 507.261 kb on + strandat 507.261 kb on + strandat 507.261 kb on + strandat 507.262 kb on - strandat 507.262 kb on - strandat 507.272 kb on - strandat 507.272 kb on - strandat 507.337 kb on + strand, within Pf1N1B4_6045at 507.338 kb on - strand, within Pf1N1B4_6045at 507.406 kb on + strand, within Pf1N1B4_6045at 507.407 kb on - strandat 507.407 kb on - strand, within Pf1N1B4_6045at 507.407 kb on - strand, within Pf1N1B4_6045

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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504,369 + +0.0
504,370 - +1.6
504,400 + +0.0
504,427 + Pf1N1B4_6041 0.17 +0.0
504,428 - Pf1N1B4_6041 0.18 +0.0
504,446 + Pf1N1B4_6041 0.27 +0.0
504,447 - Pf1N1B4_6041 0.28 +0.0
504,711 - +0.0
504,854 - Pf1N1B4_6042 0.16 +0.0
504,872 + +1.0
504,873 - Pf1N1B4_6042 0.20 -3.5
504,873 - Pf1N1B4_6042 0.20 +0.0
504,873 - Pf1N1B4_6042 0.20 -1.4
505,015 - Pf1N1B4_6042 0.51 +0.0
505,015 - Pf1N1B4_6042 0.51 -3.3
505,018 - Pf1N1B4_6042 0.52 +0.0
505,018 - Pf1N1B4_6042 0.52 +0.0
505,018 - Pf1N1B4_6042 0.52 -3.7
505,152 - Pf1N1B4_6042 0.81 +0.0
505,152 - Pf1N1B4_6042 0.81 +0.0
505,244 - +0.0
505,244 - -3.6
505,244 - +0.0
505,244 - +0.0
505,244 - +0.0
505,244 - +0.0
505,251 - +0.0
505,273 + +0.0
505,273 + -2.2
505,274 - +0.0
505,274 - -5.2
505,274 - -0.9
505,274 - +0.0
505,278 + -3.3
505,281 + -2.9
505,281 + +0.0
505,282 - +0.0
505,282 - +0.0
505,282 - +0.0
505,282 - -3.6
505,282 - +0.0
505,282 - -3.9
505,297 - +0.0
505,297 - +0.0
505,297 - -3.7
505,297 - +0.7
505,363 + -2.0
505,364 - +0.0
505,364 - +0.0
505,378 - +0.0
505,378 - +0.0
505,378 - -1.8
505,378 - -2.3
505,580 - Pf1N1B4_6043 0.19 -2.8
505,616 - Pf1N1B4_6043 0.22 -2.5
505,637 - Pf1N1B4_6043 0.24 +0.0
505,637 - Pf1N1B4_6043 0.24 -1.7
505,714 + -0.9
505,729 + Pf1N1B4_6043 0.31 +0.0
505,730 - Pf1N1B4_6043 0.31 -3.1
505,730 - Pf1N1B4_6043 0.31 +0.0
505,735 + Pf1N1B4_6043 0.32 +0.0
505,736 - Pf1N1B4_6043 0.32 +0.0
505,736 - +0.0
505,753 + Pf1N1B4_6043 0.33 -1.8
506,083 + Pf1N1B4_6043 0.60 +0.0
506,084 - Pf1N1B4_6043 0.60 -2.1
506,084 - Pf1N1B4_6043 0.60 +0.0
506,104 + Pf1N1B4_6043 0.62 +0.0
506,104 + Pf1N1B4_6043 0.62 +0.0
506,104 + Pf1N1B4_6043 0.62 +0.0
506,105 - Pf1N1B4_6043 0.62 -3.0
506,105 - Pf1N1B4_6043 0.62 +0.0
506,186 + -3.1
506,206 - Pf1N1B4_6043 0.70 +0.0
506,206 - Pf1N1B4_6043 0.70 -2.0
506,206 - Pf1N1B4_6043 0.70 +0.0
506,206 - Pf1N1B4_6043 0.70 +0.0
506,309 + Pf1N1B4_6043 0.79 +0.0
506,309 + Pf1N1B4_6043 0.79 -3.5
506,309 + Pf1N1B4_6043 0.79 -3.8
506,310 - -3.3
506,310 - Pf1N1B4_6043 0.79 -0.6
506,310 - Pf1N1B4_6043 0.79 -2.8
506,311 + Pf1N1B4_6043 0.79 +0.0
506,311 + Pf1N1B4_6043 0.79 -1.7
506,311 + Pf1N1B4_6043 0.79 +0.0
506,311 + Pf1N1B4_6043 0.79 +0.0
506,311 + Pf1N1B4_6043 0.79 +0.0
506,311 + Pf1N1B4_6043 0.79 +0.0
506,311 + Pf1N1B4_6043 0.79 +0.0
506,311 + Pf1N1B4_6043 0.79 +0.0
506,312 - Pf1N1B4_6043 0.79 +0.0
506,312 - Pf1N1B4_6043 0.79 -2.3
506,312 - -4.5
506,312 - Pf1N1B4_6043 0.79 -2.2
506,312 - Pf1N1B4_6043 0.79 +0.0
506,312 - Pf1N1B4_6043 0.79 +0.0
506,315 + Pf1N1B4_6043 0.79 -2.7
506,315 + Pf1N1B4_6043 0.79 +0.0
506,315 + Pf1N1B4_6043 0.79 +0.0
506,315 + Pf1N1B4_6043 0.79 +0.0
506,315 + Pf1N1B4_6043 0.79 -1.3
506,315 + Pf1N1B4_6043 0.79 +0.0
506,315 + Pf1N1B4_6043 0.79 +0.0
506,316 - Pf1N1B4_6043 0.79 -1.6
506,316 - Pf1N1B4_6043 0.79 +0.0
506,316 - Pf1N1B4_6043 0.79 -3.5
506,316 - Pf1N1B4_6043 0.79 +0.0
506,316 - Pf1N1B4_6043 0.79 -2.1
506,563 + -2.4
506,563 + +0.0
506,563 + +0.0
506,563 + +0.0
506,563 + +0.0
506,564 - +0.0
506,666 + +0.0
506,667 - -1.3
507,153 + +0.0
507,165 + -1.9
507,165 + +0.0
507,165 + +0.0
507,166 - -2.0
507,166 - +0.0
507,261 + +0.0
507,261 + +0.0
507,261 + +0.0
507,262 - -4.0
507,262 - +0.0
507,272 - -2.0
507,272 - +0.0
507,337 + Pf1N1B4_6045 0.12 -2.6
507,338 - Pf1N1B4_6045 0.12 +0.0
507,406 + Pf1N1B4_6045 0.41 -4.7
507,407 - +0.0
507,407 - Pf1N1B4_6045 0.41 -2.5
507,407 - Pf1N1B4_6045 0.41 -1.4

Or see this region's nucleotide sequence