Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5859 and Pf1N1B4_5860 are separated by 213 nucleotides Pf1N1B4_5860 and Pf1N1B4_5861 are separated by 555 nucleotides
Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657
_5859
Pf1N1B4_5860 - Two-component response regulator, at 293,871 to 294,806
_5860
Pf1N1B4_5861 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 295,362 to 296,288
_5861
Position (kb) 293
294
295 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 293.067 kb on + strand, within Pf1N1B4_5859 at 293.068 kb on - strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.201 kb on - strand at 293.201 kb on - strand, within Pf1N1B4_5859 at 293.534 kb on - strand at 293.695 kb on + strand at 293.695 kb on + strand at 293.793 kb on + strand at 293.793 kb on + strand at 294.061 kb on + strand, within Pf1N1B4_5860 at 294.198 kb on + strand, within Pf1N1B4_5860 at 294.199 kb on - strand, within Pf1N1B4_5860 at 294.251 kb on + strand, within Pf1N1B4_5860 at 294.252 kb on - strand, within Pf1N1B4_5860 at 294.499 kb on + strand, within Pf1N1B4_5860 at 294.562 kb on - strand at 295.007 kb on + strand at 295.051 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.139 kb on + strand at 295.140 kb on - strand at 295.140 kb on - strand at 295.440 kb on + strand at 295.441 kb on - strand at 295.575 kb on + strand, within Pf1N1B4_5861 at 295.641 kb on - strand, within Pf1N1B4_5861 at 295.756 kb on + strand, within Pf1N1B4_5861 at 295.757 kb on - strand, within Pf1N1B4_5861
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 293,067 + Pf1N1B4_5859 0.52 -3.5 293,068 - Pf1N1B4_5859 0.52 -3.3 293,200 + Pf1N1B4_5859 0.63 +0.0 293,200 + Pf1N1B4_5859 0.63 +0.0 293,200 + Pf1N1B4_5859 0.63 -2.3 293,200 + Pf1N1B4_5859 0.63 +0.0 293,200 + Pf1N1B4_5859 0.63 -1.6 293,200 + Pf1N1B4_5859 0.63 -2.4 293,201 - +0.0 293,201 - Pf1N1B4_5859 0.63 +0.0 293,534 - +0.0 293,695 + +0.0 293,695 + +0.0 293,793 + +0.0 293,793 + +0.0 294,061 + Pf1N1B4_5860 0.20 -1.6 294,198 + Pf1N1B4_5860 0.35 +0.0 294,199 - Pf1N1B4_5860 0.35 -0.8 294,251 + Pf1N1B4_5860 0.41 +0.0 294,252 - Pf1N1B4_5860 0.41 +0.0 294,499 + Pf1N1B4_5860 0.67 +0.0 294,562 - +0.0 295,007 + +0.0 295,051 + +0.0 295,093 + -3.7 295,093 + +0.0 295,093 + -3.9 295,093 + +0.0 295,093 + +0.0 295,093 + +0.0 295,093 + +0.0 295,093 + +0.0 295,093 + +0.0 295,093 + -4.6 295,094 - -1.6 295,094 - +0.0 295,094 - +0.0 295,094 - -2.8 295,094 - -2.2 295,094 - +0.0 295,094 - +0.0 295,094 - +0.0 295,094 - +0.0 295,139 + -4.8 295,140 - +0.0 295,140 - -1.5 295,440 + +0.0 295,441 - -3.0 295,575 + Pf1N1B4_5861 0.23 +0.0 295,641 - Pf1N1B4_5861 0.30 -1.2 295,756 + Pf1N1B4_5861 0.43 -1.5 295,757 - Pf1N1B4_5861 0.43 +0.0
Or see this region's nucleotide sequence