Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5764

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5763 and Pf1N1B4_5764 are separated by 199 nucleotidesPf1N1B4_5764 and Pf1N1B4_5765 are separated by 24 nucleotidesPf1N1B4_5765 and Pf1N1B4_5766 are separated by 16 nucleotidesPf1N1B4_5766 and Pf1N1B4_5767 overlap by 4 nucleotides Pf1N1B4_5763 - Nitric oxide reductase activation protein NorQ, at 202,142 to 202,933 _5763 Pf1N1B4_5764 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 203,133 to 204,824 _5764 Pf1N1B4_5765 - membrane c-type cytochrome cy, at 204,849 to 205,163 _5765 Pf1N1B4_5766 - Cytochrome c55X precursor NirC, at 205,180 to 205,506 _5766 Pf1N1B4_5767 - Heme d1 biosynthesis protein NirF, at 205,503 to 206,681 _5767 Position (kb) 203 204 205Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 202.270 kb on + strand, within Pf1N1B4_5763at 202.501 kb on - strand, within Pf1N1B4_5763at 202.764 kb on + strand, within Pf1N1B4_5763at 202.902 kb on + strandat 202.902 kb on + strandat 202.903 kb on - strandat 202.903 kb on - strandat 203.053 kb on - strandat 203.053 kb on - strandat 203.069 kb on - strandat 203.436 kb on + strand, within Pf1N1B4_5764at 203.437 kb on - strand, within Pf1N1B4_5764at 203.437 kb on - strand, within Pf1N1B4_5764at 203.908 kb on - strand, within Pf1N1B4_5764at 203.908 kb on - strand, within Pf1N1B4_5764at 203.908 kb on - strand, within Pf1N1B4_5764at 204.019 kb on - strand, within Pf1N1B4_5764at 204.148 kb on - strandat 204.148 kb on - strand, within Pf1N1B4_5764at 204.148 kb on - strand, within Pf1N1B4_5764at 204.148 kb on - strand, within Pf1N1B4_5764at 204.148 kb on - strand, within Pf1N1B4_5764at 204.148 kb on - strand, within Pf1N1B4_5764at 204.334 kb on - strand, within Pf1N1B4_5764at 204.334 kb on - strand, within Pf1N1B4_5764at 204.498 kb on + strand, within Pf1N1B4_5764at 204.499 kb on - strand, within Pf1N1B4_5764at 204.648 kb on + strand, within Pf1N1B4_5764at 204.648 kb on + strand, within Pf1N1B4_5764at 204.648 kb on + strand, within Pf1N1B4_5764at 204.754 kb on + strandat 204.755 kb on - strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.819 kb on + strandat 204.820 kb on - strandat 204.820 kb on - strandat 204.820 kb on - strandat 204.820 kb on - strandat 204.857 kb on + strandat 205.201 kb on - strandat 205.526 kb on - strandat 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strand, within Pf1N1B4_5767at 205.725 kb on + strandat 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.726 kb on - strand, within Pf1N1B4_5767at 205.796 kb on - strand, within Pf1N1B4_5767

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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202,270 + Pf1N1B4_5763 0.16 +0.0
202,501 - Pf1N1B4_5763 0.45 -5.1
202,764 + Pf1N1B4_5763 0.79 +0.0
202,902 + -4.1
202,902 + -6.6
202,903 - -4.3
202,903 - -3.8
203,053 - +0.0
203,053 - +0.0
203,069 - +0.0
203,436 + Pf1N1B4_5764 0.18 +0.4
203,437 - Pf1N1B4_5764 0.18 +1.2
203,437 - Pf1N1B4_5764 0.18 +0.5
203,908 - Pf1N1B4_5764 0.46 +0.0
203,908 - Pf1N1B4_5764 0.46 +0.0
203,908 - Pf1N1B4_5764 0.46 +1.8
204,019 - Pf1N1B4_5764 0.52 +1.4
204,148 - +0.0
204,148 - Pf1N1B4_5764 0.60 +0.0
204,148 - Pf1N1B4_5764 0.60 -1.6
204,148 - Pf1N1B4_5764 0.60 +0.0
204,148 - Pf1N1B4_5764 0.60 -0.0
204,148 - Pf1N1B4_5764 0.60 +0.1
204,334 - Pf1N1B4_5764 0.71 -0.2
204,334 - Pf1N1B4_5764 0.71 +1.1
204,498 + Pf1N1B4_5764 0.81 +0.4
204,499 - Pf1N1B4_5764 0.81 +1.1
204,648 + Pf1N1B4_5764 0.90 +0.0
204,648 + Pf1N1B4_5764 0.90 +0.0
204,648 + Pf1N1B4_5764 0.90 +0.2
204,754 + +0.0
204,755 - +0.3
204,819 + +0.0
204,819 + +0.0
204,819 + -0.8
204,819 + +0.0
204,820 - +0.0
204,820 - +0.0
204,820 - +1.5
204,820 - +0.0
204,857 + -1.0
205,201 - +0.0
205,526 - +0.0
205,725 + Pf1N1B4_5767 0.19 +0.7
205,725 + Pf1N1B4_5767 0.19 +0.0
205,725 + Pf1N1B4_5767 0.19 +0.0
205,725 + +0.0
205,726 - Pf1N1B4_5767 0.19 +1.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.0
205,726 - Pf1N1B4_5767 0.19 +0.4
205,796 - Pf1N1B4_5767 0.25 +0.0

Or see this region's nucleotide sequence