Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4930 and Pf1N1B4_4931 are separated by 252 nucleotides Pf1N1B4_4931 and Pf1N1B4_4932 are separated by 40 nucleotides
Pf1N1B4_4930 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,463,792 to 5,464,949
_4930
Pf1N1B4_4931 - Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 5,465,202 to 5,466,659
_4931
Pf1N1B4_4932 - Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83), at 5,466,700 to 5,467,779
_4932
Position (kb)
5465
5466
5467 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5464.327 kb on - strand, within Pf1N1B4_4930 at 5464.831 kb on + strand at 5464.831 kb on + strand, within Pf1N1B4_4930 at 5464.831 kb on + strand, within Pf1N1B4_4930 at 5464.832 kb on - strand, within Pf1N1B4_4930 at 5465.170 kb on - strand at 5465.250 kb on + strand at 5465.251 kb on - strand at 5465.251 kb on - strand at 5465.251 kb on - strand at 5466.114 kb on + strand, within Pf1N1B4_4931 at 5466.115 kb on - strand, within Pf1N1B4_4931 at 5466.457 kb on - strand at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.606 kb on + strand at 5466.622 kb on - strand at 5466.646 kb on + strand at 5466.647 kb on - strand at 5466.647 kb on - strand at 5466.824 kb on + strand, within Pf1N1B4_4932 at 5466.847 kb on + strand at 5467.006 kb on + strand, within Pf1N1B4_4932 at 5467.117 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.568 kb on - strand, within Pf1N1B4_4932
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 5,464,327 - Pf1N1B4_4930 0.46 +0.1 5,464,831 + +0.1 5,464,831 + Pf1N1B4_4930 0.90 -2.7 5,464,831 + Pf1N1B4_4930 0.90 +0.1 5,464,832 - Pf1N1B4_4930 0.90 -1.8 5,465,170 - +0.1 5,465,250 + +0.1 5,465,251 - +0.1 5,465,251 - +0.1 5,465,251 - +0.1 5,466,114 + Pf1N1B4_4931 0.63 +0.1 5,466,115 - Pf1N1B4_4931 0.63 -5.1 5,466,457 - +0.1 5,466,460 - Pf1N1B4_4931 0.86 +0.1 5,466,460 - Pf1N1B4_4931 0.86 -3.2 5,466,606 + +0.1 5,466,622 - -2.7 5,466,646 + -1.3 5,466,647 - -1.4 5,466,647 - +0.1 5,466,824 + Pf1N1B4_4932 0.11 -2.2 5,466,847 + +0.1 5,467,006 + Pf1N1B4_4932 0.28 +0.1 5,467,117 + Pf1N1B4_4932 0.39 +0.1 5,467,567 + Pf1N1B4_4932 0.80 +0.1 5,467,567 + Pf1N1B4_4932 0.80 -2.3 5,467,568 - Pf1N1B4_4932 0.80 -0.6
Or see this region's nucleotide sequence