Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_47 and Pf1N1B4_48 are separated by 145 nucleotides Pf1N1B4_48 and Pf1N1B4_49 are separated by 30 nucleotides Pf1N1B4_49 and Pf1N1B4_50 are separated by 163 nucleotides Pf1N1B4_50 and Pf1N1B4_51 are separated by 89 nucleotides
Pf1N1B4_47 - Copper resistance protein D, at 54,921 to 55,850
_47
Pf1N1B4_48 - hypothetical protein, at 55,996 to 56,406
_48
Pf1N1B4_49 - hypothetical protein, at 56,437 to 56,643
_49
Pf1N1B4_50 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 56,807 to 56,989
_50
Pf1N1B4_51 - hypothetical protein, at 57,079 to 57,348
_51
Position (kb) 55
56
57 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 55.006 kb on - strand at 55.006 kb on - strand at 55.014 kb on - strand, within Pf1N1B4_47 at 55.276 kb on + strand, within Pf1N1B4_47 at 55.277 kb on - strand, within Pf1N1B4_47 at 55.341 kb on - strand, within Pf1N1B4_47 at 55.499 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.612 kb on - strand, within Pf1N1B4_47 at 55.848 kb on + strand at 55.848 kb on + strand at 55.863 kb on + strand at 55.863 kb on + strand at 55.864 kb on - strand at 55.870 kb on + strand at 55.980 kb on + strand at 55.980 kb on + strand at 55.981 kb on - strand at 56.005 kb on + strand at 56.006 kb on - strand at 56.516 kb on + strand, within Pf1N1B4_49 at 56.517 kb on - strand, within Pf1N1B4_49 at 56.520 kb on - strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.560 kb on - strand, within Pf1N1B4_49 at 56.560 kb on - strand at 56.560 kb on - strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.767 kb on + strand at 56.784 kb on + strand at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.896 kb on - strand at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 57.074 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 55,006 - -2.6 55,006 - +1.1 55,014 - Pf1N1B4_47 0.10 +0.1 55,276 + Pf1N1B4_47 0.38 +0.1 55,277 - Pf1N1B4_47 0.38 +0.1 55,341 - Pf1N1B4_47 0.45 +0.1 55,499 + Pf1N1B4_47 0.62 +0.1 55,611 + Pf1N1B4_47 0.74 +0.1 55,611 + Pf1N1B4_47 0.74 -1.1 55,611 + Pf1N1B4_47 0.74 -1.2 55,611 + Pf1N1B4_47 0.74 -3.3 55,612 - Pf1N1B4_47 0.74 +0.1 55,848 + +0.1 55,848 + +0.1 55,863 + +0.1 55,863 + +0.1 55,864 - +0.1 55,870 + +0.1 55,980 + +0.1 55,980 + -4.7 55,981 - +0.1 56,005 + +0.1 56,006 - +0.1 56,516 + Pf1N1B4_49 0.38 +0.1 56,517 - Pf1N1B4_49 0.39 +0.1 56,520 - Pf1N1B4_49 0.40 +0.1 56,559 + Pf1N1B4_49 0.59 -3.0 56,559 + Pf1N1B4_49 0.59 +0.1 56,560 - Pf1N1B4_49 0.59 +0.1 56,560 - +0.1 56,560 - Pf1N1B4_49 0.59 +0.1 56,588 + Pf1N1B4_49 0.73 -2.4 56,588 + Pf1N1B4_49 0.73 -2.6 56,767 + -0.4 56,784 + +0.1 56,837 + Pf1N1B4_50 0.16 +0.1 56,837 + Pf1N1B4_50 0.16 +0.1 56,837 + Pf1N1B4_50 0.16 -1.9 56,837 + Pf1N1B4_50 0.16 +0.1 56,838 - Pf1N1B4_50 0.17 +0.1 56,838 - Pf1N1B4_50 0.17 +0.1 56,838 - Pf1N1B4_50 0.17 -2.7 56,838 - -2.7 56,895 + Pf1N1B4_50 0.48 +0.1 56,895 + +0.1 56,895 + Pf1N1B4_50 0.48 -2.4 56,895 + Pf1N1B4_50 0.48 +0.1 56,895 + Pf1N1B4_50 0.48 -3.4 56,895 + Pf1N1B4_50 0.48 +0.1 56,896 - -3.5 56,896 - Pf1N1B4_50 0.49 +0.1 56,896 - Pf1N1B4_50 0.49 +0.1 56,896 - Pf1N1B4_50 0.49 -2.3 56,896 - Pf1N1B4_50 0.49 +0.1 57,074 + +0.1 57,087 + -1.9 57,087 + +0.1 57,087 + +0.1 57,087 + +0.1 57,221 + Pf1N1B4_51 0.53 +0.1 57,221 + Pf1N1B4_51 0.53 +0.1 57,221 + -3.4 57,221 + Pf1N1B4_51 0.53 +0.1 57,221 + Pf1N1B4_51 0.53 +0.1 57,221 + Pf1N1B4_51 0.53 -2.7 57,221 + Pf1N1B4_51 0.53 -1.4 57,222 - Pf1N1B4_51 0.53 +0.1 57,222 - Pf1N1B4_51 0.53 +0.1
Or see this region's nucleotide sequence