Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4651 and Pf1N1B4_4652 are separated by 70 nucleotides Pf1N1B4_4652 and Pf1N1B4_4653 are separated by 126 nucleotides Pf1N1B4_4653 and Pf1N1B4_4654 are separated by 15 nucleotides Pf1N1B4_4654 and Pf1N1B4_4655 are separated by 15 nucleotides Pf1N1B4_4655 and Pf1N1B4_4656 are separated by 213 nucleotides
Pf1N1B4_4651 - Transcriptional regulator, AraC family, at 5,130,336 to 5,131,112
_4651
Pf1N1B4_4652 - FIG00958406: hypothetical protein, at 5,131,183 to 5,131,677
_4652
Pf1N1B4_4653 - FIG00960119: hypothetical protein, at 5,131,804 to 5,132,025
_4653
Pf1N1B4_4654 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 5,132,041 to 5,133,507
_4654
Pf1N1B4_4655 - hypothetical protein, at 5,133,523 to 5,133,636
_4655
Pf1N1B4_4656 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 5,133,850 to 5,135,523
_4656
Position (kb)
5132
5133
5134 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5131.111 kb on - strand at 5131.222 kb on + strand at 5131.222 kb on + strand at 5131.222 kb on + strand at 5131.370 kb on - strand, within Pf1N1B4_4652 at 5132.003 kb on - strand at 5132.109 kb on - strand at 5132.282 kb on + strand, within Pf1N1B4_4654 at 5132.379 kb on - strand, within Pf1N1B4_4654 at 5132.454 kb on - strand, within Pf1N1B4_4654 at 5132.516 kb on + strand, within Pf1N1B4_4654 at 5132.569 kb on - strand, within Pf1N1B4_4654 at 5132.715 kb on - strand, within Pf1N1B4_4654 at 5132.715 kb on - strand, within Pf1N1B4_4654 at 5132.762 kb on + strand, within Pf1N1B4_4654 at 5132.762 kb on + strand at 5132.795 kb on + strand, within Pf1N1B4_4654 at 5132.795 kb on + strand at 5132.795 kb on + strand, within Pf1N1B4_4654 at 5132.796 kb on - strand, within Pf1N1B4_4654 at 5132.798 kb on + strand at 5132.798 kb on + strand, within Pf1N1B4_4654 at 5132.798 kb on + strand, within Pf1N1B4_4654 at 5132.799 kb on - strand, within Pf1N1B4_4654 at 5132.799 kb on - strand, within Pf1N1B4_4654 at 5132.799 kb on - strand at 5132.799 kb on - strand, within Pf1N1B4_4654 at 5132.853 kb on - strand, within Pf1N1B4_4654 at 5132.888 kb on + strand, within Pf1N1B4_4654 at 5132.993 kb on + strand, within Pf1N1B4_4654 at 5132.993 kb on + strand, within Pf1N1B4_4654 at 5133.230 kb on + strand, within Pf1N1B4_4654 at 5133.233 kb on + strand, within Pf1N1B4_4654 at 5133.233 kb on + strand, within Pf1N1B4_4654 at 5133.233 kb on + strand, within Pf1N1B4_4654 at 5133.402 kb on + strand at 5133.403 kb on - strand at 5133.403 kb on - strand at 5133.434 kb on + strand at 5133.434 kb on + strand at 5133.434 kb on + strand at 5133.435 kb on - strand at 5133.435 kb on - strand at 5133.438 kb on + strand at 5133.440 kb on + strand at 5133.440 kb on + strand at 5133.441 kb on - strand at 5133.441 kb on - strand at 5133.441 kb on - strand at 5133.532 kb on - strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.854 kb on - strand at 5133.854 kb on - strand at 5133.854 kb on - strand at 5133.854 kb on - strand at 5133.859 kb on + strand at 5133.869 kb on - strand at 5133.985 kb on + strand at 5133.985 kb on + strand at 5133.986 kb on - strand at 5133.986 kb on - strand at 5134.003 kb on + strand at 5134.004 kb on - strand at 5134.081 kb on + strand, within Pf1N1B4_4656 at 5134.082 kb on - strand, within Pf1N1B4_4656 at 5134.411 kb on + strand, within Pf1N1B4_4656 at 5134.418 kb on - strand, within Pf1N1B4_4656
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 5,131,111 - -2.8 5,131,222 + -3.4 5,131,222 + -2.1 5,131,222 + +0.1 5,131,370 - Pf1N1B4_4652 0.38 +0.1 5,132,003 - -2.5 5,132,109 - +0.1 5,132,282 + Pf1N1B4_4654 0.16 +0.1 5,132,379 - Pf1N1B4_4654 0.23 +0.1 5,132,454 - Pf1N1B4_4654 0.28 +0.1 5,132,516 + Pf1N1B4_4654 0.32 -1.4 5,132,569 - Pf1N1B4_4654 0.36 -1.9 5,132,715 - Pf1N1B4_4654 0.46 -2.8 5,132,715 - Pf1N1B4_4654 0.46 +0.1 5,132,762 + Pf1N1B4_4654 0.49 +0.1 5,132,762 + +0.1 5,132,795 + Pf1N1B4_4654 0.51 +0.1 5,132,795 + -2.3 5,132,795 + Pf1N1B4_4654 0.51 -2.5 5,132,796 - Pf1N1B4_4654 0.51 -1.9 5,132,798 + -1.9 5,132,798 + Pf1N1B4_4654 0.52 -1.9 5,132,798 + Pf1N1B4_4654 0.52 -2.0 5,132,799 - Pf1N1B4_4654 0.52 +0.1 5,132,799 - Pf1N1B4_4654 0.52 +0.1 5,132,799 - -0.8 5,132,799 - Pf1N1B4_4654 0.52 -2.2 5,132,853 - Pf1N1B4_4654 0.55 -1.8 5,132,888 + Pf1N1B4_4654 0.58 +0.1 5,132,993 + Pf1N1B4_4654 0.65 +0.1 5,132,993 + Pf1N1B4_4654 0.65 -3.5 5,133,230 + Pf1N1B4_4654 0.81 -1.5 5,133,233 + Pf1N1B4_4654 0.81 -1.3 5,133,233 + Pf1N1B4_4654 0.81 +0.1 5,133,233 + Pf1N1B4_4654 0.81 -0.7 5,133,402 + +0.1 5,133,403 - +0.1 5,133,403 - -0.4 5,133,434 + +0.1 5,133,434 + +0.3 5,133,434 + +0.1 5,133,435 - -1.9 5,133,435 - -1.6 5,133,438 + -0.3 5,133,440 + +0.1 5,133,440 + +0.1 5,133,441 - +0.1 5,133,441 - -2.4 5,133,441 - +0.1 5,133,532 - +0.1 5,133,676 + +0.1 5,133,676 + +0.8 5,133,676 + +0.6 5,133,676 + +0.1 5,133,676 + +0.1 5,133,853 + +0.1 5,133,853 + +0.1 5,133,853 + -2.4 5,133,853 + +0.1 5,133,853 + -2.4 5,133,854 - -3.0 5,133,854 - +0.1 5,133,854 - -2.5 5,133,854 - -2.0 5,133,859 + +0.1 5,133,869 - +0.1 5,133,985 + -1.2 5,133,985 + -1.5 5,133,986 - +0.1 5,133,986 - +0.1 5,134,003 + -2.3 5,134,004 - -3.8 5,134,081 + Pf1N1B4_4656 0.14 +0.1 5,134,082 - Pf1N1B4_4656 0.14 -2.6 5,134,411 + Pf1N1B4_4656 0.34 +0.1 5,134,418 - Pf1N1B4_4656 0.34 -2.5
Or see this region's nucleotide sequence