Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4654

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4651 and Pf1N1B4_4652 are separated by 70 nucleotidesPf1N1B4_4652 and Pf1N1B4_4653 are separated by 126 nucleotidesPf1N1B4_4653 and Pf1N1B4_4654 are separated by 15 nucleotidesPf1N1B4_4654 and Pf1N1B4_4655 are separated by 15 nucleotidesPf1N1B4_4655 and Pf1N1B4_4656 are separated by 213 nucleotides Pf1N1B4_4651 - Transcriptional regulator, AraC family, at 5,130,336 to 5,131,112 _4651 Pf1N1B4_4652 - FIG00958406: hypothetical protein, at 5,131,183 to 5,131,677 _4652 Pf1N1B4_4653 - FIG00960119: hypothetical protein, at 5,131,804 to 5,132,025 _4653 Pf1N1B4_4654 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 5,132,041 to 5,133,507 _4654 Pf1N1B4_4655 - hypothetical protein, at 5,133,523 to 5,133,636 _4655 Pf1N1B4_4656 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 5,133,850 to 5,135,523 _4656 Position (kb) 5132 5133 5134Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5131.111 kb on - strandat 5131.222 kb on + strandat 5131.222 kb on + strandat 5131.222 kb on + strandat 5131.370 kb on - strand, within Pf1N1B4_4652at 5132.003 kb on - strandat 5132.109 kb on - strandat 5132.282 kb on + strand, within Pf1N1B4_4654at 5132.379 kb on - strand, within Pf1N1B4_4654at 5132.454 kb on - strand, within Pf1N1B4_4654at 5132.516 kb on + strand, within Pf1N1B4_4654at 5132.569 kb on - strand, within Pf1N1B4_4654at 5132.715 kb on - strand, within Pf1N1B4_4654at 5132.715 kb on - strand, within Pf1N1B4_4654at 5132.762 kb on + strand, within Pf1N1B4_4654at 5132.762 kb on + strandat 5132.795 kb on + strand, within Pf1N1B4_4654at 5132.795 kb on + strandat 5132.795 kb on + strand, within Pf1N1B4_4654at 5132.796 kb on - strand, within Pf1N1B4_4654at 5132.798 kb on + strandat 5132.798 kb on + strand, within Pf1N1B4_4654at 5132.798 kb on + strand, within Pf1N1B4_4654at 5132.799 kb on - strand, within Pf1N1B4_4654at 5132.799 kb on - strand, within Pf1N1B4_4654at 5132.799 kb on - strandat 5132.799 kb on - strand, within Pf1N1B4_4654at 5132.853 kb on - strand, within Pf1N1B4_4654at 5132.888 kb on + strand, within Pf1N1B4_4654at 5132.993 kb on + strand, within Pf1N1B4_4654at 5132.993 kb on + strand, within Pf1N1B4_4654at 5133.230 kb on + strand, within Pf1N1B4_4654at 5133.233 kb on + strand, within Pf1N1B4_4654at 5133.233 kb on + strand, within Pf1N1B4_4654at 5133.233 kb on + strand, within Pf1N1B4_4654at 5133.402 kb on + strandat 5133.403 kb on - strandat 5133.403 kb on - strandat 5133.434 kb on + strandat 5133.434 kb on + strandat 5133.434 kb on + strandat 5133.435 kb on - strandat 5133.435 kb on - strandat 5133.438 kb on + strandat 5133.440 kb on + strandat 5133.440 kb on + strandat 5133.441 kb on - strandat 5133.441 kb on - strandat 5133.441 kb on - strandat 5133.532 kb on - strandat 5133.676 kb on + strandat 5133.676 kb on + strandat 5133.676 kb on + strandat 5133.676 kb on + strandat 5133.676 kb on + strandat 5133.853 kb on + strandat 5133.853 kb on + strandat 5133.853 kb on + strandat 5133.853 kb on + strandat 5133.853 kb on + strandat 5133.854 kb on - strandat 5133.854 kb on - strandat 5133.854 kb on - strandat 5133.854 kb on - strandat 5133.859 kb on + strandat 5133.869 kb on - strandat 5133.985 kb on + strandat 5133.985 kb on + strandat 5133.986 kb on - strandat 5133.986 kb on - strandat 5134.003 kb on + strandat 5134.004 kb on - strandat 5134.081 kb on + strand, within Pf1N1B4_4656at 5134.082 kb on - strand, within Pf1N1B4_4656at 5134.411 kb on + strand, within Pf1N1B4_4656at 5134.418 kb on - strand, within Pf1N1B4_4656

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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5,131,111 - -2.8
5,131,222 + -3.4
5,131,222 + -2.1
5,131,222 + +0.1
5,131,370 - Pf1N1B4_4652 0.38 +0.1
5,132,003 - -2.5
5,132,109 - +0.1
5,132,282 + Pf1N1B4_4654 0.16 +0.1
5,132,379 - Pf1N1B4_4654 0.23 +0.1
5,132,454 - Pf1N1B4_4654 0.28 +0.1
5,132,516 + Pf1N1B4_4654 0.32 -1.4
5,132,569 - Pf1N1B4_4654 0.36 -1.9
5,132,715 - Pf1N1B4_4654 0.46 -2.8
5,132,715 - Pf1N1B4_4654 0.46 +0.1
5,132,762 + Pf1N1B4_4654 0.49 +0.1
5,132,762 + +0.1
5,132,795 + Pf1N1B4_4654 0.51 +0.1
5,132,795 + -2.3
5,132,795 + Pf1N1B4_4654 0.51 -2.5
5,132,796 - Pf1N1B4_4654 0.51 -1.9
5,132,798 + -1.9
5,132,798 + Pf1N1B4_4654 0.52 -1.9
5,132,798 + Pf1N1B4_4654 0.52 -2.0
5,132,799 - Pf1N1B4_4654 0.52 +0.1
5,132,799 - Pf1N1B4_4654 0.52 +0.1
5,132,799 - -0.8
5,132,799 - Pf1N1B4_4654 0.52 -2.2
5,132,853 - Pf1N1B4_4654 0.55 -1.8
5,132,888 + Pf1N1B4_4654 0.58 +0.1
5,132,993 + Pf1N1B4_4654 0.65 +0.1
5,132,993 + Pf1N1B4_4654 0.65 -3.5
5,133,230 + Pf1N1B4_4654 0.81 -1.5
5,133,233 + Pf1N1B4_4654 0.81 -1.3
5,133,233 + Pf1N1B4_4654 0.81 +0.1
5,133,233 + Pf1N1B4_4654 0.81 -0.7
5,133,402 + +0.1
5,133,403 - +0.1
5,133,403 - -0.4
5,133,434 + +0.1
5,133,434 + +0.3
5,133,434 + +0.1
5,133,435 - -1.9
5,133,435 - -1.6
5,133,438 + -0.3
5,133,440 + +0.1
5,133,440 + +0.1
5,133,441 - +0.1
5,133,441 - -2.4
5,133,441 - +0.1
5,133,532 - +0.1
5,133,676 + +0.1
5,133,676 + +0.8
5,133,676 + +0.6
5,133,676 + +0.1
5,133,676 + +0.1
5,133,853 + +0.1
5,133,853 + +0.1
5,133,853 + -2.4
5,133,853 + +0.1
5,133,853 + -2.4
5,133,854 - -3.0
5,133,854 - +0.1
5,133,854 - -2.5
5,133,854 - -2.0
5,133,859 + +0.1
5,133,869 - +0.1
5,133,985 + -1.2
5,133,985 + -1.5
5,133,986 - +0.1
5,133,986 - +0.1
5,134,003 + -2.3
5,134,004 - -3.8
5,134,081 + Pf1N1B4_4656 0.14 +0.1
5,134,082 - Pf1N1B4_4656 0.14 -2.6
5,134,411 + Pf1N1B4_4656 0.34 +0.1
5,134,418 - Pf1N1B4_4656 0.34 -2.5

Or see this region's nucleotide sequence