Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4527 and Pf1N1B4_4528 are separated by 24 nucleotides Pf1N1B4_4528 and Pf1N1B4_4529 are separated by 190 nucleotides Pf1N1B4_4529 and Pf1N1B4_4530 are separated by 336 nucleotides
Pf1N1B4_4527 - Short-chain dehydrogenase/reductase SDR, at 4,991,158 to 4,991,925
_4527
Pf1N1B4_4528 - Predicted aminoglycoside phosphotransferase, at 4,991,950 to 4,993,017
_4528
Pf1N1B4_4529 - FIG00966904: hypothetical protein, at 4,993,208 to 4,993,603
_4529
Pf1N1B4_4530 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 4,993,940 to 4,994,482
_4530
Position (kb)
4991
4992
4993
4994 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4990.963 kb on + strand at 4990.964 kb on - strand at 4990.978 kb on - strand at 4990.978 kb on - strand at 4991.105 kb on - strand at 4991.105 kb on - strand at 4991.204 kb on + strand at 4991.205 kb on - strand at 4991.228 kb on + strand at 4991.228 kb on + strand at 4991.228 kb on + strand at 4991.228 kb on + strand at 4991.229 kb on - strand at 4991.318 kb on - strand, within Pf1N1B4_4527 at 4991.444 kb on + strand, within Pf1N1B4_4527 at 4991.444 kb on + strand, within Pf1N1B4_4527 at 4991.444 kb on + strand, within Pf1N1B4_4527 at 4991.445 kb on - strand at 4991.445 kb on - strand at 4991.445 kb on - strand at 4991.445 kb on - strand, within Pf1N1B4_4527 at 4991.472 kb on + strand, within Pf1N1B4_4527 at 4991.472 kb on + strand at 4991.473 kb on - strand, within Pf1N1B4_4527 at 4992.062 kb on + strand, within Pf1N1B4_4528 at 4992.062 kb on + strand, within Pf1N1B4_4528 at 4992.063 kb on - strand, within Pf1N1B4_4528 at 4992.063 kb on - strand, within Pf1N1B4_4528 at 4992.101 kb on + strand at 4992.107 kb on + strand, within Pf1N1B4_4528 at 4992.108 kb on - strand, within Pf1N1B4_4528 at 4992.110 kb on + strand, within Pf1N1B4_4528 at 4992.111 kb on - strand at 4992.152 kb on + strand, within Pf1N1B4_4528 at 4992.447 kb on + strand at 4992.512 kb on + strand, within Pf1N1B4_4528 at 4992.555 kb on - strand, within Pf1N1B4_4528 at 4992.686 kb on + strand, within Pf1N1B4_4528 at 4992.728 kb on + strand, within Pf1N1B4_4528 at 4992.728 kb on + strand, within Pf1N1B4_4528 at 4992.729 kb on - strand, within Pf1N1B4_4528 at 4992.729 kb on - strand, within Pf1N1B4_4528 at 4993.008 kb on + strand at 4993.030 kb on + strand at 4993.065 kb on + strand at 4993.106 kb on + strand at 4993.106 kb on + strand at 4993.106 kb on + strand at 4993.106 kb on + strand at 4993.106 kb on + strand at 4993.107 kb on - strand at 4993.107 kb on - strand at 4993.107 kb on - strand at 4993.167 kb on - strand at 4993.273 kb on - strand, within Pf1N1B4_4529 at 4993.739 kb on + strand at 4993.868 kb on - strand at 4993.868 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 4,990,963 + -3.6 4,990,964 - +0.1 4,990,978 - -2.3 4,990,978 - +0.1 4,991,105 - +0.1 4,991,105 - +0.1 4,991,204 + +0.1 4,991,205 - +0.1 4,991,228 + -3.5 4,991,228 + +0.1 4,991,228 + -2.4 4,991,228 + -1.4 4,991,229 - -2.1 4,991,318 - Pf1N1B4_4527 0.21 +0.1 4,991,444 + Pf1N1B4_4527 0.37 -3.4 4,991,444 + Pf1N1B4_4527 0.37 -2.2 4,991,444 + Pf1N1B4_4527 0.37 +0.1 4,991,445 - +0.1 4,991,445 - +0.1 4,991,445 - -1.9 4,991,445 - Pf1N1B4_4527 0.37 +0.1 4,991,472 + Pf1N1B4_4527 0.41 -2.9 4,991,472 + +0.1 4,991,473 - Pf1N1B4_4527 0.41 +0.1 4,992,062 + Pf1N1B4_4528 0.10 +0.1 4,992,062 + Pf1N1B4_4528 0.10 -0.7 4,992,063 - Pf1N1B4_4528 0.11 -1.3 4,992,063 - Pf1N1B4_4528 0.11 +0.1 4,992,101 + +0.1 4,992,107 + Pf1N1B4_4528 0.15 +0.1 4,992,108 - Pf1N1B4_4528 0.15 -1.4 4,992,110 + Pf1N1B4_4528 0.15 -1.6 4,992,111 - -1.4 4,992,152 + Pf1N1B4_4528 0.19 -2.3 4,992,447 + -2.1 4,992,512 + Pf1N1B4_4528 0.53 -1.1 4,992,555 - Pf1N1B4_4528 0.57 +0.1 4,992,686 + Pf1N1B4_4528 0.69 +0.1 4,992,728 + Pf1N1B4_4528 0.73 +0.1 4,992,728 + Pf1N1B4_4528 0.73 +0.1 4,992,729 - Pf1N1B4_4528 0.73 +0.1 4,992,729 - Pf1N1B4_4528 0.73 +0.1 4,993,008 + -0.7 4,993,030 + -1.8 4,993,065 + +0.1 4,993,106 + +0.1 4,993,106 + +0.1 4,993,106 + -2.8 4,993,106 + -0.9 4,993,106 + +0.1 4,993,107 - +0.1 4,993,107 - -2.1 4,993,107 - -3.0 4,993,167 - -1.5 4,993,273 - Pf1N1B4_4529 0.16 +0.1 4,993,739 + +0.1 4,993,868 - -2.4 4,993,868 - -1.9
Or see this region's nucleotide sequence