Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4092 and Pf1N1B4_4093 are separated by 159 nucleotides Pf1N1B4_4093 and Pf1N1B4_4094 are separated by 225 nucleotides Pf1N1B4_4094 and Pf1N1B4_4095 are separated by 10 nucleotides
Pf1N1B4_4092 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 4,540,762 to 4,542,174
_4092
Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987
_4093
Pf1N1B4_4094 - Uncharacterized conserved protein, at 4,543,213 to 4,543,710
_4094
Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722
_4095
Position (kb)
4542
4543 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.444 kb on - strand, within Pf1N1B4_4092 at 4541.630 kb on - strand, within Pf1N1B4_4092 at 4541.630 kb on - strand at 4541.833 kb on + strand at 4541.834 kb on - strand, within Pf1N1B4_4092 at 4541.894 kb on - strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.896 kb on + strand, within Pf1N1B4_4092 at 4541.897 kb on - strand, within Pf1N1B4_4092 at 4541.897 kb on - strand, within Pf1N1B4_4092 at 4541.969 kb on + strand, within Pf1N1B4_4092 at 4541.969 kb on + strand, within Pf1N1B4_4092 at 4541.986 kb on + strand, within Pf1N1B4_4092 at 4541.986 kb on + strand, within Pf1N1B4_4092 at 4542.072 kb on + strand at 4542.112 kb on + strand at 4542.115 kb on + strand at 4542.240 kb on + strand at 4542.240 kb on + strand at 4542.243 kb on - strand at 4542.307 kb on + strand at 4542.320 kb on + strand at 4542.320 kb on + strand at 4542.484 kb on + strand, within Pf1N1B4_4093 at 4542.656 kb on - strand, within Pf1N1B4_4093 at 4542.712 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.929 kb on - strand at 4542.958 kb on + strand at 4542.959 kb on - strand at 4543.021 kb on + strand at 4543.167 kb on + strand at 4543.167 kb on + strand at 4543.176 kb on - strand at 4543.176 kb on - strand at 4543.380 kb on - strand, within Pf1N1B4_4094 at 4543.445 kb on - strand, within Pf1N1B4_4094 at 4543.536 kb on - strand, within Pf1N1B4_4094 at 4543.928 kb on - strand, within Pf1N1B4_4095
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 4,541,423 - Pf1N1B4_4092 0.47 +0.1 4,541,423 - Pf1N1B4_4092 0.47 +0.1 4,541,423 - Pf1N1B4_4092 0.47 -2.3 4,541,423 - -3.1 4,541,423 - Pf1N1B4_4092 0.47 +0.1 4,541,444 - Pf1N1B4_4092 0.48 -2.3 4,541,630 - Pf1N1B4_4092 0.61 -2.3 4,541,630 - -4.3 4,541,833 + +0.1 4,541,834 - Pf1N1B4_4092 0.76 +0.1 4,541,894 - Pf1N1B4_4092 0.80 -1.8 4,541,896 + Pf1N1B4_4092 0.80 -0.5 4,541,896 + Pf1N1B4_4092 0.80 +0.1 4,541,896 + Pf1N1B4_4092 0.80 +0.1 4,541,897 - Pf1N1B4_4092 0.80 -0.7 4,541,897 - Pf1N1B4_4092 0.80 -1.8 4,541,969 + Pf1N1B4_4092 0.85 -3.8 4,541,969 + Pf1N1B4_4092 0.85 -1.8 4,541,986 + Pf1N1B4_4092 0.87 +0.1 4,541,986 + Pf1N1B4_4092 0.87 +0.1 4,542,072 + +0.1 4,542,112 + +0.1 4,542,115 + +0.1 4,542,240 + +0.1 4,542,240 + -1.1 4,542,243 - +0.1 4,542,307 + -2.0 4,542,320 + +0.1 4,542,320 + -3.6 4,542,484 + Pf1N1B4_4093 0.23 +0.1 4,542,656 - Pf1N1B4_4093 0.49 -3.2 4,542,712 - Pf1N1B4_4093 0.58 +0.1 4,542,770 - Pf1N1B4_4093 0.67 +0.1 4,542,770 - Pf1N1B4_4093 0.67 +0.1 4,542,929 - +0.1 4,542,958 + +0.1 4,542,959 - +0.1 4,543,021 + +0.1 4,543,167 + +0.1 4,543,167 + +0.1 4,543,176 - -3.3 4,543,176 - -2.6 4,543,380 - Pf1N1B4_4094 0.34 -1.4 4,543,445 - Pf1N1B4_4094 0.47 +0.1 4,543,536 - Pf1N1B4_4094 0.65 -2.4 4,543,928 - Pf1N1B4_4095 0.21 +0.1
Or see this region's nucleotide sequence