Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4090 and Pf1N1B4_4091 are separated by 111 nucleotides Pf1N1B4_4091 and Pf1N1B4_4092 are separated by 196 nucleotides
Pf1N1B4_4090 - Exoenzymes regulatory protein AepA in lipid-linked oligosaccharide synthesis cluster, at 4,537,971 to 4,539,713
_4090
Pf1N1B4_4091 - Probable signal peptide protein, at 4,539,825 to 4,540,565
_4091
Pf1N1B4_4092 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 4,540,762 to 4,542,174
_4092
Position (kb)
4539
4540
4541 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4539.303 kb on + strand, within Pf1N1B4_4090 at 4539.303 kb on + strand, within Pf1N1B4_4090 at 4539.304 kb on - strand, within Pf1N1B4_4090 at 4539.304 kb on - strand, within Pf1N1B4_4090 at 4539.327 kb on + strand, within Pf1N1B4_4090 at 4539.327 kb on + strand, within Pf1N1B4_4090 at 4539.329 kb on + strand, within Pf1N1B4_4090 at 4539.329 kb on + strand, within Pf1N1B4_4090 at 4539.482 kb on - strand, within Pf1N1B4_4090 at 4539.523 kb on - strand, within Pf1N1B4_4090 at 4539.528 kb on + strand, within Pf1N1B4_4090 at 4539.528 kb on + strand, within Pf1N1B4_4090 at 4539.529 kb on - strand, within Pf1N1B4_4090 at 4539.696 kb on + strand at 4539.697 kb on - strand at 4539.808 kb on - strand at 4539.808 kb on - strand at 4539.824 kb on + strand at 4539.825 kb on - strand at 4539.825 kb on - strand at 4539.825 kb on - strand at 4539.832 kb on + strand at 4539.832 kb on + strand at 4539.862 kb on + strand at 4539.862 kb on + strand at 4539.862 kb on + strand at 4539.863 kb on - strand at 4539.863 kb on - strand at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.024 kb on - strand, within Pf1N1B4_4091 at 4540.102 kb on - strand, within Pf1N1B4_4091 at 4540.102 kb on - strand, within Pf1N1B4_4091 at 4540.144 kb on - strand, within Pf1N1B4_4091 at 4540.152 kb on + strand, within Pf1N1B4_4091 at 4540.153 kb on - strand, within Pf1N1B4_4091 at 4540.285 kb on - strand, within Pf1N1B4_4091 at 4540.291 kb on - strand at 4540.291 kb on - strand, within Pf1N1B4_4091 at 4540.291 kb on - strand, within Pf1N1B4_4091 at 4540.291 kb on - strand, within Pf1N1B4_4091 at 4540.299 kb on + strand, within Pf1N1B4_4091 at 4540.404 kb on + strand at 4540.405 kb on - strand, within Pf1N1B4_4091 at 4540.405 kb on - strand, within Pf1N1B4_4091 at 4540.405 kb on - strand, within Pf1N1B4_4091 at 4540.464 kb on + strand, within Pf1N1B4_4091 at 4540.464 kb on + strand, within Pf1N1B4_4091 at 4540.563 kb on + strand at 4540.563 kb on + strand at 4540.563 kb on + strand at 4540.564 kb on - strand at 4540.590 kb on + strand at 4540.591 kb on - strand at 4540.760 kb on + strand at 4540.822 kb on + strand at 4540.822 kb on + strand at 4540.823 kb on - strand at 4541.071 kb on + strand at 4541.297 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.423 kb on - strand at 4541.423 kb on - strand, within Pf1N1B4_4092 at 4541.444 kb on - strand, within Pf1N1B4_4092
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 4,539,303 + Pf1N1B4_4090 0.76 +0.1 4,539,303 + Pf1N1B4_4090 0.76 -2.8 4,539,304 - Pf1N1B4_4090 0.76 -2.3 4,539,304 - Pf1N1B4_4090 0.76 +0.1 4,539,327 + Pf1N1B4_4090 0.78 -1.7 4,539,327 + Pf1N1B4_4090 0.78 +0.1 4,539,329 + Pf1N1B4_4090 0.78 -1.8 4,539,329 + Pf1N1B4_4090 0.78 -1.3 4,539,482 - Pf1N1B4_4090 0.87 +0.1 4,539,523 - Pf1N1B4_4090 0.89 +0.1 4,539,528 + Pf1N1B4_4090 0.89 +0.1 4,539,528 + Pf1N1B4_4090 0.89 +0.1 4,539,529 - Pf1N1B4_4090 0.89 -3.3 4,539,696 + +0.1 4,539,697 - +0.1 4,539,808 - -2.2 4,539,808 - -2.3 4,539,824 + +0.1 4,539,825 - -2.2 4,539,825 - +0.1 4,539,825 - -1.7 4,539,832 + +0.1 4,539,832 + +0.1 4,539,862 + -1.0 4,539,862 + -3.5 4,539,862 + -1.7 4,539,863 - +0.1 4,539,863 - +0.1 4,540,024 - Pf1N1B4_4091 0.27 +0.1 4,540,024 - Pf1N1B4_4091 0.27 +0.1 4,540,024 - Pf1N1B4_4091 0.27 +0.1 4,540,024 - Pf1N1B4_4091 0.27 +0.3 4,540,102 - Pf1N1B4_4091 0.37 +0.1 4,540,102 - Pf1N1B4_4091 0.37 -2.0 4,540,144 - Pf1N1B4_4091 0.43 -3.3 4,540,152 + Pf1N1B4_4091 0.44 -2.1 4,540,153 - Pf1N1B4_4091 0.44 +0.1 4,540,285 - Pf1N1B4_4091 0.62 +0.1 4,540,291 - +0.1 4,540,291 - Pf1N1B4_4091 0.63 +0.1 4,540,291 - Pf1N1B4_4091 0.63 +0.1 4,540,291 - Pf1N1B4_4091 0.63 +0.1 4,540,299 + Pf1N1B4_4091 0.64 -2.1 4,540,404 + -2.5 4,540,405 - Pf1N1B4_4091 0.78 -1.7 4,540,405 - Pf1N1B4_4091 0.78 +0.1 4,540,405 - Pf1N1B4_4091 0.78 +0.1 4,540,464 + Pf1N1B4_4091 0.86 +0.1 4,540,464 + Pf1N1B4_4091 0.86 +0.1 4,540,563 + +0.1 4,540,563 + +0.1 4,540,563 + +0.1 4,540,564 - +0.1 4,540,590 + -1.7 4,540,591 - +0.1 4,540,760 + +0.1 4,540,822 + -2.7 4,540,822 + +0.1 4,540,823 - +0.1 4,541,071 + -2.1 4,541,297 - Pf1N1B4_4092 0.38 +0.1 4,541,423 - Pf1N1B4_4092 0.47 +0.1 4,541,423 - Pf1N1B4_4092 0.47 +0.1 4,541,423 - Pf1N1B4_4092 0.47 -2.3 4,541,423 - -3.1 4,541,423 - Pf1N1B4_4092 0.47 +0.1 4,541,444 - Pf1N1B4_4092 0.48 -2.3
Or see this region's nucleotide sequence