Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3957

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3956 and Pf1N1B4_3957 are separated by 170 nucleotidesPf1N1B4_3957 and Pf1N1B4_3958 are separated by 190 nucleotides Pf1N1B4_3956 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 4,395,335 to 4,396,030 _3956 Pf1N1B4_3957 - cytochrome b(561), at 4,396,201 to 4,396,749 _3957 Pf1N1B4_3958 - Phosphonoacetaldehyde hydrolase (EC 3.11.1.1), at 4,396,940 to 4,397,767 _3958 Position (kb) 4396 4397Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4395.523 kb on - strand, within Pf1N1B4_3956at 4395.523 kb on - strand, within Pf1N1B4_3956at 4395.627 kb on - strand, within Pf1N1B4_3956at 4395.707 kb on + strand, within Pf1N1B4_3956at 4395.708 kb on - strand, within Pf1N1B4_3956at 4395.759 kb on + strandat 4395.759 kb on + strand, within Pf1N1B4_3956at 4395.759 kb on + strand, within Pf1N1B4_3956at 4395.759 kb on + strand, within Pf1N1B4_3956at 4395.759 kb on + strand, within Pf1N1B4_3956at 4395.759 kb on + strand, within Pf1N1B4_3956at 4395.759 kb on + strand, within Pf1N1B4_3956at 4395.760 kb on - strand, within Pf1N1B4_3956at 4395.760 kb on - strand, within Pf1N1B4_3956at 4395.760 kb on - strandat 4395.794 kb on + strand, within Pf1N1B4_3956at 4395.902 kb on + strand, within Pf1N1B4_3956at 4396.043 kb on + strandat 4396.043 kb on + strandat 4396.250 kb on + strandat 4396.251 kb on - strandat 4396.251 kb on - strandat 4396.251 kb on - strandat 4396.251 kb on - strandat 4396.251 kb on - strandat 4396.251 kb on - strandat 4396.260 kb on - strandat 4396.260 kb on - strand, within Pf1N1B4_3957at 4396.260 kb on - strand, within Pf1N1B4_3957at 4396.385 kb on + strand, within Pf1N1B4_3957at 4396.386 kb on - strand, within Pf1N1B4_3957at 4396.386 kb on - strand, within Pf1N1B4_3957at 4396.455 kb on + strand, within Pf1N1B4_3957at 4396.456 kb on - strand, within Pf1N1B4_3957at 4396.456 kb on - strand, within Pf1N1B4_3957at 4396.458 kb on - strand, within Pf1N1B4_3957at 4396.458 kb on - strandat 4396.664 kb on + strand, within Pf1N1B4_3957at 4396.665 kb on - strand, within Pf1N1B4_3957at 4396.665 kb on - strand, within Pf1N1B4_3957at 4396.682 kb on - strand, within Pf1N1B4_3957at 4396.711 kb on + strandat 4396.711 kb on + strandat 4396.722 kb on - strandat 4396.749 kb on + strandat 4396.749 kb on + strandat 4396.813 kb on + strandat 4396.813 kb on + strandat 4396.813 kb on + strandat 4396.813 kb on + strandat 4396.813 kb on + strandat 4396.872 kb on - strandat 4396.872 kb on - strandat 4397.028 kb on + strandat 4397.028 kb on + strand, within Pf1N1B4_3958at 4397.028 kb on + strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.029 kb on - strand, within Pf1N1B4_3958at 4397.107 kb on - strand, within Pf1N1B4_3958at 4397.116 kb on - strand, within Pf1N1B4_3958at 4397.326 kb on - strand, within Pf1N1B4_3958at 4397.499 kb on + strand, within Pf1N1B4_3958at 4397.499 kb on + strand, within Pf1N1B4_3958at 4397.500 kb on - strand, within Pf1N1B4_3958at 4397.500 kb on - strand, within Pf1N1B4_3958

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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4,395,523 - Pf1N1B4_3956 0.27 +0.0
4,395,523 - Pf1N1B4_3956 0.27 +0.0
4,395,627 - Pf1N1B4_3956 0.42 -2.0
4,395,707 + Pf1N1B4_3956 0.53 -2.0
4,395,708 - Pf1N1B4_3956 0.54 +0.0
4,395,759 + -2.6
4,395,759 + Pf1N1B4_3956 0.61 +0.0
4,395,759 + Pf1N1B4_3956 0.61 +0.0
4,395,759 + Pf1N1B4_3956 0.61 +0.0
4,395,759 + Pf1N1B4_3956 0.61 +0.0
4,395,759 + Pf1N1B4_3956 0.61 +0.0
4,395,759 + Pf1N1B4_3956 0.61 +0.0
4,395,760 - Pf1N1B4_3956 0.61 +0.0
4,395,760 - Pf1N1B4_3956 0.61 +0.0
4,395,760 - +0.0
4,395,794 + Pf1N1B4_3956 0.66 -3.9
4,395,902 + Pf1N1B4_3956 0.81 -2.6
4,396,043 + -3.2
4,396,043 + +0.0
4,396,250 + -3.5
4,396,251 - -2.3
4,396,251 - +0.0
4,396,251 - +0.0
4,396,251 - +0.0
4,396,251 - -3.0
4,396,251 - +0.0
4,396,260 - -3.9
4,396,260 - Pf1N1B4_3957 0.11 +0.0
4,396,260 - Pf1N1B4_3957 0.11 -2.3
4,396,385 + Pf1N1B4_3957 0.34 -2.2
4,396,386 - Pf1N1B4_3957 0.34 +0.0
4,396,386 - Pf1N1B4_3957 0.34 -3.1
4,396,455 + Pf1N1B4_3957 0.46 +0.0
4,396,456 - Pf1N1B4_3957 0.46 +0.0
4,396,456 - Pf1N1B4_3957 0.46 -4.0
4,396,458 - Pf1N1B4_3957 0.47 -4.1
4,396,458 - +0.0
4,396,664 + Pf1N1B4_3957 0.84 -2.7
4,396,665 - Pf1N1B4_3957 0.85 -2.4
4,396,665 - Pf1N1B4_3957 0.85 -2.0
4,396,682 - Pf1N1B4_3957 0.88 -2.8
4,396,711 + -2.4
4,396,711 + -2.2
4,396,722 - -1.8
4,396,749 + -2.6
4,396,749 + +1.0
4,396,813 + +0.0
4,396,813 + +0.0
4,396,813 + +0.0
4,396,813 + +0.0
4,396,813 + +0.0
4,396,872 - -2.5
4,396,872 - +0.0
4,397,028 + -2.1
4,397,028 + Pf1N1B4_3958 0.11 -2.3
4,397,028 + Pf1N1B4_3958 0.11 -3.7
4,397,029 - Pf1N1B4_3958 0.11 +0.0
4,397,029 - Pf1N1B4_3958 0.11 -3.7
4,397,029 - Pf1N1B4_3958 0.11 -4.0
4,397,029 - Pf1N1B4_3958 0.11 +0.0
4,397,029 - Pf1N1B4_3958 0.11 -4.5
4,397,029 - Pf1N1B4_3958 0.11 +0.0
4,397,029 - Pf1N1B4_3958 0.11 -3.3
4,397,029 - Pf1N1B4_3958 0.11 +0.0
4,397,029 - Pf1N1B4_3958 0.11 +0.0
4,397,107 - Pf1N1B4_3958 0.20 +0.0
4,397,116 - Pf1N1B4_3958 0.21 -1.6
4,397,326 - Pf1N1B4_3958 0.47 +0.0
4,397,499 + Pf1N1B4_3958 0.68 +0.0
4,397,499 + Pf1N1B4_3958 0.68 -1.7
4,397,500 - Pf1N1B4_3958 0.68 -1.6
4,397,500 - Pf1N1B4_3958 0.68 -2.7

Or see this region's nucleotide sequence