Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3882

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3881 and Pf1N1B4_3882 overlap by 4 nucleotidesPf1N1B4_3882 and Pf1N1B4_3883 are separated by 45 nucleotidesPf1N1B4_3883 and Pf1N1B4_3884 are separated by 10 nucleotides Pf1N1B4_3881 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 4,310,112 to 4,311,065 _3881 Pf1N1B4_3882 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 4,311,062 to 4,311,238 _3882 Pf1N1B4_3883 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 4,311,284 to 4,311,892 _3883 Pf1N1B4_3884 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 4,311,903 to 4,313,327 _3884 Position (kb) 4311 4312Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4310.098 kb on - strandat 4310.147 kb on + strandat 4310.147 kb on + strandat 4310.171 kb on - strandat 4310.171 kb on - strandat 4310.171 kb on - strandat 4310.176 kb on + strandat 4310.176 kb on + strandat 4310.176 kb on + strandat 4310.176 kb on + strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.177 kb on - strandat 4310.198 kb on + strandat 4310.198 kb on + strandat 4310.231 kb on + strand, within Pf1N1B4_3881at 4310.232 kb on - strand, within Pf1N1B4_3881at 4310.232 kb on - strand, within Pf1N1B4_3881at 4310.282 kb on + strand, within Pf1N1B4_3881at 4310.283 kb on - strand, within Pf1N1B4_3881at 4310.283 kb on - strand, within Pf1N1B4_3881at 4310.283 kb on - strand, within Pf1N1B4_3881at 4310.283 kb on - strand, within Pf1N1B4_3881at 4310.283 kb on - strand, within Pf1N1B4_3881at 4310.287 kb on + strandat 4310.288 kb on - strand, within Pf1N1B4_3881at 4310.288 kb on - strand, within Pf1N1B4_3881at 4310.288 kb on - strand, within Pf1N1B4_3881at 4310.288 kb on - strand, within Pf1N1B4_3881at 4310.288 kb on - strand, within Pf1N1B4_3881at 4310.288 kb on - strand, within Pf1N1B4_3881at 4310.292 kb on - strand, within Pf1N1B4_3881at 4310.292 kb on - strand, within Pf1N1B4_3881at 4310.292 kb on - strand, within Pf1N1B4_3881at 4310.292 kb on - strand, within Pf1N1B4_3881at 4310.292 kb on - strand, within Pf1N1B4_3881at 4310.292 kb on - strand, within Pf1N1B4_3881at 4310.372 kb on + strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strandat 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strand, within Pf1N1B4_3881at 4310.373 kb on - strandat 4310.455 kb on + strand, within Pf1N1B4_3881at 4310.455 kb on + strand, within Pf1N1B4_3881at 4310.455 kb on + strand, within Pf1N1B4_3881at 4310.455 kb on + strand, within Pf1N1B4_3881at 4310.455 kb on + strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4310.456 kb on - strand, within Pf1N1B4_3881at 4311.047 kb on + strandat 4311.048 kb on - strandat 4311.251 kb on + strandat 4311.252 kb on - strandat 4311.258 kb on - strandat 4311.325 kb on - strandat 4311.325 kb on - strandat 4311.397 kb on - strand, within Pf1N1B4_3883at 4311.478 kb on - strand, within Pf1N1B4_3883at 4311.519 kb on + strand, within Pf1N1B4_3883at 4311.519 kb on + strand, within Pf1N1B4_3883at 4311.520 kb on - strand, within Pf1N1B4_3883at 4311.520 kb on - strand, within Pf1N1B4_3883at 4311.520 kb on - strandat 4311.520 kb on - strand, within Pf1N1B4_3883at 4311.639 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.717 kb on + strand, within Pf1N1B4_3883at 4311.718 kb on - strand, within Pf1N1B4_3883at 4311.718 kb on - strand, within Pf1N1B4_3883at 4311.750 kb on + strand, within Pf1N1B4_3883at 4311.751 kb on - strand, within Pf1N1B4_3883at 4311.970 kb on + strandat 4311.970 kb on + strandat 4311.971 kb on - strandat 4311.971 kb on - strandat 4311.971 kb on - strandat 4312.066 kb on + strand, within Pf1N1B4_3884at 4312.066 kb on + strand, within Pf1N1B4_3884at 4312.067 kb on - strand, within Pf1N1B4_3884at 4312.067 kb on - strand, within Pf1N1B4_3884at 4312.067 kb on - strand, within Pf1N1B4_3884at 4312.067 kb on - strand, within Pf1N1B4_3884at 4312.067 kb on - strand, within Pf1N1B4_3884at 4312.144 kb on + strand, within Pf1N1B4_3884at 4312.144 kb on + strandat 4312.145 kb on - strand, within Pf1N1B4_3884at 4312.145 kb on - strandat 4312.145 kb on - strand, within Pf1N1B4_3884

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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4,310,098 - +0.0
4,310,147 + -4.2
4,310,147 + +0.0
4,310,171 - +0.0
4,310,171 - -3.7
4,310,171 - +0.0
4,310,176 + -2.9
4,310,176 + +0.0
4,310,176 + +0.0
4,310,176 + +0.0
4,310,177 - +0.0
4,310,177 - +0.0
4,310,177 - +0.1
4,310,177 - +0.0
4,310,177 - +0.3
4,310,177 - +0.0
4,310,177 - +0.0
4,310,177 - +0.0
4,310,198 + -1.7
4,310,198 + +0.0
4,310,231 + Pf1N1B4_3881 0.12 -2.6
4,310,232 - Pf1N1B4_3881 0.13 +0.0
4,310,232 - Pf1N1B4_3881 0.13 +0.0
4,310,282 + Pf1N1B4_3881 0.18 +0.0
4,310,283 - Pf1N1B4_3881 0.18 +0.0
4,310,283 - Pf1N1B4_3881 0.18 +0.0
4,310,283 - Pf1N1B4_3881 0.18 +0.4
4,310,283 - Pf1N1B4_3881 0.18 -0.1
4,310,283 - Pf1N1B4_3881 0.18 +0.0
4,310,287 + +0.0
4,310,288 - Pf1N1B4_3881 0.18 +0.0
4,310,288 - Pf1N1B4_3881 0.18 -3.4
4,310,288 - Pf1N1B4_3881 0.18 +0.0
4,310,288 - Pf1N1B4_3881 0.18 +0.1
4,310,288 - Pf1N1B4_3881 0.18 +0.0
4,310,288 - Pf1N1B4_3881 0.18 +0.0
4,310,292 - Pf1N1B4_3881 0.19 +0.0
4,310,292 - Pf1N1B4_3881 0.19 -1.2
4,310,292 - Pf1N1B4_3881 0.19 +0.0
4,310,292 - Pf1N1B4_3881 0.19 +0.3
4,310,292 - Pf1N1B4_3881 0.19 +0.0
4,310,292 - Pf1N1B4_3881 0.19 -0.0
4,310,372 + Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.3
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - +0.0
4,310,373 - Pf1N1B4_3881 0.27 -0.4
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 -0.5
4,310,373 - Pf1N1B4_3881 0.27 -0.4
4,310,373 - Pf1N1B4_3881 0.27 +0.0
4,310,373 - Pf1N1B4_3881 0.27 -0.2
4,310,373 - +0.0
4,310,455 + Pf1N1B4_3881 0.36 +0.0
4,310,455 + Pf1N1B4_3881 0.36 +0.0
4,310,455 + Pf1N1B4_3881 0.36 +0.0
4,310,455 + Pf1N1B4_3881 0.36 -2.4
4,310,455 + Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 -0.2
4,310,456 - Pf1N1B4_3881 0.36 -0.2
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 -0.9
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 +1.1
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 +0.0
4,310,456 - Pf1N1B4_3881 0.36 +0.1
4,311,047 + -1.3
4,311,048 - +0.0
4,311,251 + +0.0
4,311,252 - +0.0
4,311,258 - +0.0
4,311,325 - +0.0
4,311,325 - +0.0
4,311,397 - Pf1N1B4_3883 0.19 -0.2
4,311,478 - Pf1N1B4_3883 0.32 +0.0
4,311,519 + Pf1N1B4_3883 0.39 +0.0
4,311,519 + Pf1N1B4_3883 0.39 -1.4
4,311,520 - Pf1N1B4_3883 0.39 +0.0
4,311,520 - Pf1N1B4_3883 0.39 +0.0
4,311,520 - -0.6
4,311,520 - Pf1N1B4_3883 0.39 +0.0
4,311,639 + Pf1N1B4_3883 0.58 +0.0
4,311,717 + Pf1N1B4_3883 0.71 -1.8
4,311,717 + Pf1N1B4_3883 0.71 -0.5
4,311,717 + Pf1N1B4_3883 0.71 +0.0
4,311,717 + Pf1N1B4_3883 0.71 +0.0
4,311,717 + Pf1N1B4_3883 0.71 -1.7
4,311,717 + Pf1N1B4_3883 0.71 +0.0
4,311,717 + Pf1N1B4_3883 0.71 +0.0
4,311,718 - Pf1N1B4_3883 0.71 +0.0
4,311,718 - Pf1N1B4_3883 0.71 +0.0
4,311,750 + Pf1N1B4_3883 0.77 -0.8
4,311,751 - Pf1N1B4_3883 0.77 +0.0
4,311,970 + -1.0
4,311,970 + -1.8
4,311,971 - -2.2
4,311,971 - +0.0
4,311,971 - +0.0
4,312,066 + Pf1N1B4_3884 0.11 +0.0
4,312,066 + Pf1N1B4_3884 0.11 -3.7
4,312,067 - Pf1N1B4_3884 0.12 +0.0
4,312,067 - Pf1N1B4_3884 0.12 +0.0
4,312,067 - Pf1N1B4_3884 0.12 +0.0
4,312,067 - Pf1N1B4_3884 0.12 -3.4
4,312,067 - Pf1N1B4_3884 0.12 +0.0
4,312,144 + Pf1N1B4_3884 0.17 -1.2
4,312,144 + +0.0
4,312,145 - Pf1N1B4_3884 0.17 +0.0
4,312,145 - -2.4
4,312,145 - Pf1N1B4_3884 0.17 -0.6

Or see this region's nucleotide sequence