Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3704

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3702 and Pf1N1B4_3703 are separated by 165 nucleotidesPf1N1B4_3703 and Pf1N1B4_3704 are separated by 164 nucleotidesPf1N1B4_3704 and Pf1N1B4_3705 are separated by 68 nucleotides Pf1N1B4_3702 - NLP/P60 family protein, at 4,111,158 to 4,111,721 _3702 Pf1N1B4_3703 - NLP/P60 family protein, at 4,111,887 to 4,112,423 _3703 Pf1N1B4_3704 - L-sorbosone dehydrogenase, at 4,112,588 to 4,113,901 _3704 Pf1N1B4_3705 - FIG00961250: hypothetical protein, at 4,113,970 to 4,114,554 _3705 Position (kb) 4112 4113 4114Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4111.635 kb on + strand, within Pf1N1B4_3702at 4111.719 kb on + strandat 4111.734 kb on + strandat 4111.746 kb on - strandat 4111.753 kb on + strandat 4111.915 kb on + strandat 4111.916 kb on - strandat 4112.280 kb on + strand, within Pf1N1B4_3703at 4112.280 kb on + strand, within Pf1N1B4_3703at 4112.280 kb on + strand, within Pf1N1B4_3703at 4112.280 kb on + strandat 4112.280 kb on + strand, within Pf1N1B4_3703at 4112.280 kb on + strand, within Pf1N1B4_3703at 4112.281 kb on - strand, within Pf1N1B4_3703at 4112.281 kb on - strandat 4112.281 kb on - strand, within Pf1N1B4_3703at 4112.356 kb on - strand, within Pf1N1B4_3703at 4112.356 kb on - strand, within Pf1N1B4_3703at 4112.370 kb on + strandat 4112.414 kb on + strandat 4112.414 kb on + strandat 4112.414 kb on + strandat 4112.522 kb on + strandat 4112.589 kb on + strandat 4112.589 kb on + strandat 4112.590 kb on - strandat 4112.590 kb on - strandat 4112.776 kb on + strandat 4112.776 kb on + strand, within Pf1N1B4_3704at 4112.776 kb on + strand, within Pf1N1B4_3704at 4112.777 kb on - strand, within Pf1N1B4_3704at 4112.777 kb on - strand, within Pf1N1B4_3704at 4112.980 kb on - strand, within Pf1N1B4_3704at 4112.980 kb on - strand, within Pf1N1B4_3704at 4112.980 kb on - strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.982 kb on + strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.983 kb on - strand, within Pf1N1B4_3704at 4112.989 kb on - strand, within Pf1N1B4_3704at 4113.030 kb on + strandat 4113.030 kb on + strand, within Pf1N1B4_3704at 4113.031 kb on - strand, within Pf1N1B4_3704at 4113.031 kb on - strand, within Pf1N1B4_3704at 4113.031 kb on - strand, within Pf1N1B4_3704at 4113.031 kb on - strand, within Pf1N1B4_3704at 4113.249 kb on + strand, within Pf1N1B4_3704at 4113.249 kb on + strand, within Pf1N1B4_3704at 4113.249 kb on + strand, within Pf1N1B4_3704at 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.250 kb on - strandat 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.250 kb on - strand, within Pf1N1B4_3704at 4113.405 kb on + strand, within Pf1N1B4_3704at 4113.405 kb on + strand, within Pf1N1B4_3704at 4113.405 kb on + strand, within Pf1N1B4_3704at 4113.405 kb on + strandat 4113.405 kb on + strand, within Pf1N1B4_3704at 4113.405 kb on + strand, within Pf1N1B4_3704at 4113.406 kb on - strand, within Pf1N1B4_3704at 4113.406 kb on - strandat 4113.406 kb on - strand, within Pf1N1B4_3704at 4113.406 kb on - strand, within Pf1N1B4_3704at 4113.461 kb on + strand, within Pf1N1B4_3704at 4113.651 kb on + strand, within Pf1N1B4_3704at 4113.651 kb on + strand, within Pf1N1B4_3704at 4113.652 kb on - strand, within Pf1N1B4_3704at 4113.652 kb on - strand, within Pf1N1B4_3704at 4113.652 kb on - strand, within Pf1N1B4_3704at 4113.652 kb on - strand, within Pf1N1B4_3704at 4113.652 kb on - strand, within Pf1N1B4_3704at 4113.652 kb on - strand, within Pf1N1B4_3704at 4113.724 kb on + strand, within Pf1N1B4_3704at 4113.724 kb on + strand, within Pf1N1B4_3704at 4113.724 kb on + strand, within Pf1N1B4_3704at 4113.724 kb on + strand, within Pf1N1B4_3704at 4113.902 kb on + strandat 4113.903 kb on - strandat 4113.915 kb on + strandat 4113.916 kb on - strandat 4113.931 kb on + strandat 4113.931 kb on + strandat 4113.932 kb on - strandat 4113.932 kb on - strandat 4113.932 kb on - strandat 4113.932 kb on - strandat 4113.937 kb on + strandat 4113.937 kb on + strandat 4113.947 kb on + strandat 4113.948 kb on - strandat 4114.013 kb on + strandat 4114.014 kb on - strandat 4114.014 kb on - strandat 4114.014 kb on - strandat 4114.014 kb on - strandat 4114.050 kb on - strand, within Pf1N1B4_3705at 4114.058 kb on + strand, within Pf1N1B4_3705at 4114.058 kb on + strand, within Pf1N1B4_3705at 4114.058 kb on + strand, within Pf1N1B4_3705at 4114.059 kb on - strand, within Pf1N1B4_3705at 4114.077 kb on + strand, within Pf1N1B4_3705at 4114.077 kb on + strand, within Pf1N1B4_3705at 4114.077 kb on + strandat 4114.077 kb on + strand, within Pf1N1B4_3705at 4114.077 kb on + strand, within Pf1N1B4_3705at 4114.077 kb on + strand, within Pf1N1B4_3705at 4114.077 kb on + strandat 4114.078 kb on - strand, within Pf1N1B4_3705at 4114.078 kb on - strand, within Pf1N1B4_3705at 4114.187 kb on + strand, within Pf1N1B4_3705at 4114.280 kb on + strand, within Pf1N1B4_3705at 4114.280 kb on + strand, within Pf1N1B4_3705at 4114.280 kb on + strand, within Pf1N1B4_3705at 4114.440 kb on - strand, within Pf1N1B4_3705at 4114.570 kb on + strandat 4114.570 kb on + strandat 4114.637 kb on + strandat 4114.638 kb on - strandat 4114.638 kb on - strandat 4114.655 kb on + strandat 4114.698 kb on + strandat 4114.846 kb on + strandat 4114.847 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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4,111,635 + Pf1N1B4_3702 0.85 -3.7
4,111,719 + +0.0
4,111,734 + +0.0
4,111,746 - +0.0
4,111,753 + +0.0
4,111,915 + +0.0
4,111,916 - -3.3
4,112,280 + Pf1N1B4_3703 0.73 +0.0
4,112,280 + Pf1N1B4_3703 0.73 +0.0
4,112,280 + Pf1N1B4_3703 0.73 +0.0
4,112,280 + -3.5
4,112,280 + Pf1N1B4_3703 0.73 +0.0
4,112,280 + Pf1N1B4_3703 0.73 +0.0
4,112,281 - Pf1N1B4_3703 0.73 +0.0
4,112,281 - +0.0
4,112,281 - Pf1N1B4_3703 0.73 +0.0
4,112,356 - Pf1N1B4_3703 0.87 +0.0
4,112,356 - Pf1N1B4_3703 0.87 +1.0
4,112,370 + -2.1
4,112,414 + +0.0
4,112,414 + +0.0
4,112,414 + -3.5
4,112,522 + +0.0
4,112,589 + -0.8
4,112,589 + -2.0
4,112,590 - +0.0
4,112,590 - +0.0
4,112,776 + +0.0
4,112,776 + Pf1N1B4_3704 0.14 +0.0
4,112,776 + Pf1N1B4_3704 0.14 +0.0
4,112,777 - Pf1N1B4_3704 0.14 +0.0
4,112,777 - Pf1N1B4_3704 0.14 -2.5
4,112,980 - Pf1N1B4_3704 0.30 +0.0
4,112,980 - Pf1N1B4_3704 0.30 +0.0
4,112,980 - Pf1N1B4_3704 0.30 +0.0
4,112,982 + Pf1N1B4_3704 0.30 +0.0
4,112,982 + Pf1N1B4_3704 0.30 -4.1
4,112,982 + Pf1N1B4_3704 0.30 -1.9
4,112,982 + Pf1N1B4_3704 0.30 +0.0
4,112,982 + Pf1N1B4_3704 0.30 +0.0
4,112,982 + Pf1N1B4_3704 0.30 +0.0
4,112,982 + Pf1N1B4_3704 0.30 -2.4
4,112,982 + Pf1N1B4_3704 0.30 +0.0
4,112,982 + Pf1N1B4_3704 0.30 -3.3
4,112,983 - Pf1N1B4_3704 0.30 +0.0
4,112,983 - Pf1N1B4_3704 0.30 +0.0
4,112,983 - Pf1N1B4_3704 0.30 +0.0
4,112,983 - Pf1N1B4_3704 0.30 -3.2
4,112,983 - Pf1N1B4_3704 0.30 -2.0
4,112,983 - Pf1N1B4_3704 0.30 +0.0
4,112,983 - Pf1N1B4_3704 0.30 -1.6
4,112,983 - Pf1N1B4_3704 0.30 +0.0
4,112,989 - Pf1N1B4_3704 0.31 +0.0
4,113,030 + -1.8
4,113,030 + Pf1N1B4_3704 0.34 -2.1
4,113,031 - Pf1N1B4_3704 0.34 +0.0
4,113,031 - Pf1N1B4_3704 0.34 -2.1
4,113,031 - Pf1N1B4_3704 0.34 -3.1
4,113,031 - Pf1N1B4_3704 0.34 -1.0
4,113,249 + Pf1N1B4_3704 0.50 +0.0
4,113,249 + Pf1N1B4_3704 0.50 +0.0
4,113,249 + Pf1N1B4_3704 0.50 -1.1
4,113,250 - Pf1N1B4_3704 0.50 +0.0
4,113,250 - Pf1N1B4_3704 0.50 +0.0
4,113,250 - Pf1N1B4_3704 0.50 -2.8
4,113,250 - -3.2
4,113,250 - Pf1N1B4_3704 0.50 +0.0
4,113,250 - Pf1N1B4_3704 0.50 +0.0
4,113,250 - Pf1N1B4_3704 0.50 -2.7
4,113,250 - Pf1N1B4_3704 0.50 +0.0
4,113,405 + Pf1N1B4_3704 0.62 +0.0
4,113,405 + Pf1N1B4_3704 0.62 +0.0
4,113,405 + Pf1N1B4_3704 0.62 -3.2
4,113,405 + -2.9
4,113,405 + Pf1N1B4_3704 0.62 +0.0
4,113,405 + Pf1N1B4_3704 0.62 +0.0
4,113,406 - Pf1N1B4_3704 0.62 +0.0
4,113,406 - +0.0
4,113,406 - Pf1N1B4_3704 0.62 +0.0
4,113,406 - Pf1N1B4_3704 0.62 -2.4
4,113,461 + Pf1N1B4_3704 0.66 -2.5
4,113,651 + Pf1N1B4_3704 0.81 +0.0
4,113,651 + Pf1N1B4_3704 0.81 +0.0
4,113,652 - Pf1N1B4_3704 0.81 +0.0
4,113,652 - Pf1N1B4_3704 0.81 -3.0
4,113,652 - Pf1N1B4_3704 0.81 +0.0
4,113,652 - Pf1N1B4_3704 0.81 +0.0
4,113,652 - Pf1N1B4_3704 0.81 -3.2
4,113,652 - Pf1N1B4_3704 0.81 -2.5
4,113,724 + Pf1N1B4_3704 0.86 -4.8
4,113,724 + Pf1N1B4_3704 0.86 +0.0
4,113,724 + Pf1N1B4_3704 0.86 +0.0
4,113,724 + Pf1N1B4_3704 0.86 +0.0
4,113,902 + +0.0
4,113,903 - -3.5
4,113,915 + -2.7
4,113,916 - +0.0
4,113,931 + +0.0
4,113,931 + -2.0
4,113,932 - +0.0
4,113,932 - -3.1
4,113,932 - -1.9
4,113,932 - +0.0
4,113,937 + -2.3
4,113,937 + -1.1
4,113,947 + +0.0
4,113,948 - -2.6
4,114,013 + +0.0
4,114,014 - -2.6
4,114,014 - +0.0
4,114,014 - +0.0
4,114,014 - -3.0
4,114,050 - Pf1N1B4_3705 0.14 -2.7
4,114,058 + Pf1N1B4_3705 0.15 +0.0
4,114,058 + Pf1N1B4_3705 0.15 +0.0
4,114,058 + Pf1N1B4_3705 0.15 -0.4
4,114,059 - Pf1N1B4_3705 0.15 -6.0
4,114,077 + Pf1N1B4_3705 0.18 -3.0
4,114,077 + Pf1N1B4_3705 0.18 +0.0
4,114,077 + +0.0
4,114,077 + Pf1N1B4_3705 0.18 -1.8
4,114,077 + Pf1N1B4_3705 0.18 -3.0
4,114,077 + Pf1N1B4_3705 0.18 -2.5
4,114,077 + +0.0
4,114,078 - Pf1N1B4_3705 0.18 +0.0
4,114,078 - Pf1N1B4_3705 0.18 +0.0
4,114,187 + Pf1N1B4_3705 0.37 +0.0
4,114,280 + Pf1N1B4_3705 0.53 +0.0
4,114,280 + Pf1N1B4_3705 0.53 +0.0
4,114,280 + Pf1N1B4_3705 0.53 +0.0
4,114,440 - Pf1N1B4_3705 0.80 -2.8
4,114,570 + -1.8
4,114,570 + -1.9
4,114,637 + +0.0
4,114,638 - +0.0
4,114,638 - +0.0
4,114,655 + -2.9
4,114,698 + -2.9
4,114,846 + +0.0
4,114,847 - +0.0

Or see this region's nucleotide sequence