Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3660 and Pf1N1B4_3661 are separated by 128 nucleotides Pf1N1B4_3661 and Pf1N1B4_3662 are separated by 6 nucleotides Pf1N1B4_3662 and Pf1N1B4_3663 overlap by 4 nucleotides
Pf1N1B4_3660 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,065,636 to 4,066,418
_3660
Pf1N1B4_3661 - Glyoxylate carboligase (EC 4.1.1.47), at 4,066,547 to 4,068,322
_3661
Pf1N1B4_3662 - hypothetical protein, at 4,068,329 to 4,068,592
_3662
Pf1N1B4_3663 - GlcG protein, at 4,068,589 to 4,068,849
_3663
Position (kb)
4066
4067
4068
4069 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4065.570 kb on + strand at 4065.647 kb on + strand at 4065.698 kb on - strand at 4065.698 kb on - strand at 4065.698 kb on - strand at 4065.880 kb on + strand, within Pf1N1B4_3660 at 4065.880 kb on + strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.881 kb on - strand, within Pf1N1B4_3660 at 4065.889 kb on + strand, within Pf1N1B4_3660 at 4065.958 kb on + strand, within Pf1N1B4_3660 at 4066.036 kb on + strand, within Pf1N1B4_3660 at 4066.037 kb on - strand, within Pf1N1B4_3660 at 4066.306 kb on + strand, within Pf1N1B4_3660 at 4066.306 kb on + strand, within Pf1N1B4_3660 at 4066.318 kb on + strand, within Pf1N1B4_3660 at 4066.318 kb on + strand, within Pf1N1B4_3660 at 4066.440 kb on - strand at 4066.440 kb on - strand at 4066.854 kb on + strand, within Pf1N1B4_3661 at 4066.854 kb on + strand, within Pf1N1B4_3661 at 4066.854 kb on + strand, within Pf1N1B4_3661 at 4066.926 kb on + strand, within Pf1N1B4_3661 at 4066.926 kb on + strand, within Pf1N1B4_3661 at 4066.927 kb on - strand, within Pf1N1B4_3661 at 4066.927 kb on - strand, within Pf1N1B4_3661 at 4066.927 kb on - strand, within Pf1N1B4_3661 at 4066.983 kb on + strand, within Pf1N1B4_3661 at 4066.983 kb on + strand, within Pf1N1B4_3661 at 4066.984 kb on - strand, within Pf1N1B4_3661 at 4066.984 kb on - strand, within Pf1N1B4_3661 at 4066.984 kb on - strand at 4066.984 kb on - strand, within Pf1N1B4_3661 at 4067.103 kb on + strand, within Pf1N1B4_3661 at 4067.104 kb on - strand, within Pf1N1B4_3661 at 4067.104 kb on - strand, within Pf1N1B4_3661 at 4067.157 kb on + strand, within Pf1N1B4_3661 at 4067.157 kb on + strand, within Pf1N1B4_3661 at 4067.158 kb on - strand, within Pf1N1B4_3661 at 4067.158 kb on - strand, within Pf1N1B4_3661 at 4067.158 kb on - strand, within Pf1N1B4_3661 at 4067.197 kb on - strand, within Pf1N1B4_3661 at 4067.197 kb on - strand, within Pf1N1B4_3661 at 4067.219 kb on + strand, within Pf1N1B4_3661 at 4067.449 kb on - strand, within Pf1N1B4_3661 at 4067.790 kb on + strand, within Pf1N1B4_3661 at 4067.791 kb on - strand, within Pf1N1B4_3661 at 4067.791 kb on - strand, within Pf1N1B4_3661 at 4067.791 kb on - strand, within Pf1N1B4_3661 at 4067.880 kb on + strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.881 kb on - strand, within Pf1N1B4_3661 at 4067.958 kb on - strand, within Pf1N1B4_3661 at 4067.958 kb on - strand, within Pf1N1B4_3661 at 4067.958 kb on - strand, within Pf1N1B4_3661 at 4068.061 kb on - strand at 4068.222 kb on + strand at 4068.353 kb on - strand at 4068.355 kb on - strand at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.377 kb on + strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.378 kb on - strand, within Pf1N1B4_3662 at 4068.599 kb on + strand at 4068.707 kb on + strand, within Pf1N1B4_3663 at 4069.096 kb on - strand at 4069.129 kb on - strand at 4069.177 kb on + strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.202 kb on + strand at 4069.203 kb on - strand at 4069.250 kb on + strand at 4069.251 kb on - strand at 4069.251 kb on - strand at 4069.269 kb on - strand at 4069.303 kb on + strand at 4069.314 kb on + strand at 4069.315 kb on - strand at 4069.315 kb on - strand at 4069.315 kb on - strand at 4069.320 kb on + strand at 4069.320 kb on + strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 4,065,570 + -3.4 4,065,647 + -2.8 4,065,698 - -3.8 4,065,698 - +0.0 4,065,698 - +0.0 4,065,880 + Pf1N1B4_3660 0.31 -2.3 4,065,880 + Pf1N1B4_3660 0.31 +0.0 4,065,881 - Pf1N1B4_3660 0.31 -3.4 4,065,881 - Pf1N1B4_3660 0.31 +0.0 4,065,881 - Pf1N1B4_3660 0.31 -2.2 4,065,881 - Pf1N1B4_3660 0.31 -3.6 4,065,881 - Pf1N1B4_3660 0.31 -1.9 4,065,881 - Pf1N1B4_3660 0.31 +0.0 4,065,889 + Pf1N1B4_3660 0.32 +0.0 4,065,958 + Pf1N1B4_3660 0.41 +0.0 4,066,036 + Pf1N1B4_3660 0.51 +0.0 4,066,037 - Pf1N1B4_3660 0.51 -2.0 4,066,306 + Pf1N1B4_3660 0.86 +0.0 4,066,306 + Pf1N1B4_3660 0.86 -3.7 4,066,318 + Pf1N1B4_3660 0.87 -2.2 4,066,318 + Pf1N1B4_3660 0.87 +0.0 4,066,440 - +0.0 4,066,440 - +0.0 4,066,854 + Pf1N1B4_3661 0.17 -1.7 4,066,854 + Pf1N1B4_3661 0.17 +0.0 4,066,854 + Pf1N1B4_3661 0.17 -1.3 4,066,926 + Pf1N1B4_3661 0.21 +0.0 4,066,926 + Pf1N1B4_3661 0.21 -2.5 4,066,927 - Pf1N1B4_3661 0.21 -1.9 4,066,927 - Pf1N1B4_3661 0.21 +0.0 4,066,927 - Pf1N1B4_3661 0.21 -1.8 4,066,983 + Pf1N1B4_3661 0.25 +0.0 4,066,983 + Pf1N1B4_3661 0.25 +0.0 4,066,984 - Pf1N1B4_3661 0.25 +0.0 4,066,984 - Pf1N1B4_3661 0.25 -1.3 4,066,984 - +0.0 4,066,984 - Pf1N1B4_3661 0.25 -1.0 4,067,103 + Pf1N1B4_3661 0.31 +0.0 4,067,104 - Pf1N1B4_3661 0.31 -2.0 4,067,104 - Pf1N1B4_3661 0.31 -2.1 4,067,157 + Pf1N1B4_3661 0.34 +0.0 4,067,157 + Pf1N1B4_3661 0.34 +0.0 4,067,158 - Pf1N1B4_3661 0.34 +0.0 4,067,158 - Pf1N1B4_3661 0.34 +0.0 4,067,158 - Pf1N1B4_3661 0.34 -2.7 4,067,197 - Pf1N1B4_3661 0.37 +0.0 4,067,197 - Pf1N1B4_3661 0.37 +0.0 4,067,219 + Pf1N1B4_3661 0.38 +0.0 4,067,449 - Pf1N1B4_3661 0.51 +0.0 4,067,790 + Pf1N1B4_3661 0.70 +0.0 4,067,791 - Pf1N1B4_3661 0.70 -2.2 4,067,791 - Pf1N1B4_3661 0.70 +0.0 4,067,791 - Pf1N1B4_3661 0.70 +0.0 4,067,880 + Pf1N1B4_3661 0.75 +0.0 4,067,881 - Pf1N1B4_3661 0.75 +0.0 4,067,881 - Pf1N1B4_3661 0.75 -3.1 4,067,881 - Pf1N1B4_3661 0.75 +0.0 4,067,881 - Pf1N1B4_3661 0.75 +0.0 4,067,881 - Pf1N1B4_3661 0.75 -2.1 4,067,958 - Pf1N1B4_3661 0.79 -1.4 4,067,958 - Pf1N1B4_3661 0.79 +0.0 4,067,958 - Pf1N1B4_3661 0.79 +0.0 4,068,061 - +0.0 4,068,222 + +0.0 4,068,353 - -1.8 4,068,355 - +0.0 4,068,377 + Pf1N1B4_3662 0.18 -3.6 4,068,377 + Pf1N1B4_3662 0.18 -2.8 4,068,377 + Pf1N1B4_3662 0.18 +0.0 4,068,377 + Pf1N1B4_3662 0.18 -2.0 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,378 - Pf1N1B4_3662 0.19 -1.7 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,378 - Pf1N1B4_3662 0.19 +0.0 4,068,599 + +0.0 4,068,707 + Pf1N1B4_3663 0.45 -1.0 4,069,096 - +0.0 4,069,129 - -2.7 4,069,177 + -3.2 4,069,178 - +0.0 4,069,178 - -1.0 4,069,178 - +0.0 4,069,178 - +0.0 4,069,178 - -3.1 4,069,178 - +0.0 4,069,178 - +0.0 4,069,178 - +0.0 4,069,202 + -0.9 4,069,203 - -2.9 4,069,250 + +0.0 4,069,251 - -3.1 4,069,251 - +0.0 4,069,269 - -1.3 4,069,303 + -2.3 4,069,314 + -0.9 4,069,315 - +0.0 4,069,315 - -2.0 4,069,315 - +0.0 4,069,320 + +0.0 4,069,320 + +0.0 4,069,321 - +0.0 4,069,321 - +0.0 4,069,321 - +0.0 4,069,321 - -2.0 4,069,321 - -2.4
Or see this region's nucleotide sequence