Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3302 and Pf1N1B4_3303 are separated by 11 nucleotides Pf1N1B4_3303 and Pf1N1B4_3304 are separated by 12 nucleotides Pf1N1B4_3304 and Pf1N1B4_3305 overlap by 4 nucleotides Pf1N1B4_3305 and Pf1N1B4_3306 are separated by 17 nucleotides
Pf1N1B4_3302 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,691,355 to 3,691,996
_3302
Pf1N1B4_3303 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,692,008 to 3,692,733
_3303
Pf1N1B4_3304 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,692,746 to 3,693,534
_3304
Pf1N1B4_3305 - Urea carboxylase-related ABC transporter, permease protein, at 3,693,531 to 3,694,346
_3305
Pf1N1B4_3306 - Urea carboxylase-related ABC transporter, periplasmic substrate-binding protein, at 3,694,364 to 3,695,431
_3306
Position (kb)
3692
3693
3694 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3691.749 kb on + strand, within Pf1N1B4_3302 at 3691.750 kb on - strand, within Pf1N1B4_3302 at 3691.773 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.813 kb on - strand, within Pf1N1B4_3302 at 3691.840 kb on - strand, within Pf1N1B4_3302 at 3692.195 kb on + strand, within Pf1N1B4_3303 at 3692.207 kb on + strand, within Pf1N1B4_3303 at 3692.208 kb on - strand, within Pf1N1B4_3303 at 3692.208 kb on - strand, within Pf1N1B4_3303 at 3692.208 kb on - strand at 3692.322 kb on + strand, within Pf1N1B4_3303 at 3692.747 kb on + strand at 3692.837 kb on + strand, within Pf1N1B4_3304 at 3692.837 kb on + strand, within Pf1N1B4_3304 at 3692.838 kb on - strand at 3692.838 kb on - strand, within Pf1N1B4_3304 at 3692.838 kb on - strand, within Pf1N1B4_3304 at 3692.855 kb on + strand at 3692.856 kb on - strand, within Pf1N1B4_3304 at 3692.856 kb on - strand, within Pf1N1B4_3304 at 3693.370 kb on - strand, within Pf1N1B4_3304 at 3693.388 kb on + strand, within Pf1N1B4_3304 at 3693.389 kb on - strand, within Pf1N1B4_3304 at 3693.391 kb on + strand, within Pf1N1B4_3304 at 3693.392 kb on - strand at 3693.485 kb on - strand at 3693.497 kb on + strand at 3693.498 kb on - strand at 3693.498 kb on - strand at 3693.498 kb on - strand at 3693.550 kb on + strand at 3693.550 kb on + strand at 3693.550 kb on + strand at 3693.551 kb on - strand at 3693.704 kb on + strand, within Pf1N1B4_3305 at 3693.704 kb on + strand, within Pf1N1B4_3305 at 3694.141 kb on + strand, within Pf1N1B4_3305 at 3694.141 kb on + strand, within Pf1N1B4_3305 at 3694.142 kb on - strand, within Pf1N1B4_3305 at 3694.142 kb on - strand, within Pf1N1B4_3305 at 3694.142 kb on - strand at 3694.142 kb on - strand, within Pf1N1B4_3305 at 3694.173 kb on + strand, within Pf1N1B4_3305 at 3694.264 kb on + strand, within Pf1N1B4_3305 at 3694.351 kb on + strand at 3694.365 kb on + strand at 3694.365 kb on + strand at 3694.365 kb on + strand at 3694.365 kb on + strand at 3694.365 kb on + strand at 3694.365 kb on + strand at 3694.365 kb on + strand at 3694.366 kb on - strand at 3694.366 kb on - strand at 3694.395 kb on + strand at 3694.395 kb on + strand at 3694.395 kb on + strand at 3694.395 kb on + strand at 3694.395 kb on + strand at 3694.395 kb on + strand at 3694.395 kb on + strand at 3694.396 kb on - strand at 3694.396 kb on - strand at 3694.396 kb on - strand at 3694.396 kb on - strand at 3694.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 3,691,749 + Pf1N1B4_3302 0.61 -1.5 3,691,750 - Pf1N1B4_3302 0.62 -0.0 3,691,773 + Pf1N1B4_3302 0.65 -4.7 3,691,805 + Pf1N1B4_3302 0.70 -2.5 3,691,805 + Pf1N1B4_3302 0.70 -1.7 3,691,805 + -0.0 3,691,805 + Pf1N1B4_3302 0.70 -0.0 3,691,805 + Pf1N1B4_3302 0.70 -2.4 3,691,806 - Pf1N1B4_3302 0.70 -0.0 3,691,806 - Pf1N1B4_3302 0.70 -0.0 3,691,806 - Pf1N1B4_3302 0.70 -0.0 3,691,806 - Pf1N1B4_3302 0.70 -1.7 3,691,813 - Pf1N1B4_3302 0.71 -0.0 3,691,840 - Pf1N1B4_3302 0.76 -1.0 3,692,195 + Pf1N1B4_3303 0.26 -0.0 3,692,207 + Pf1N1B4_3303 0.27 -0.0 3,692,208 - Pf1N1B4_3303 0.28 -0.8 3,692,208 - Pf1N1B4_3303 0.28 -2.1 3,692,208 - -0.0 3,692,322 + Pf1N1B4_3303 0.43 -3.5 3,692,747 + -0.0 3,692,837 + Pf1N1B4_3304 0.12 -0.0 3,692,837 + Pf1N1B4_3304 0.12 -2.9 3,692,838 - -4.0 3,692,838 - Pf1N1B4_3304 0.12 -0.0 3,692,838 - Pf1N1B4_3304 0.12 -2.6 3,692,855 + -4.1 3,692,856 - Pf1N1B4_3304 0.14 -2.7 3,692,856 - Pf1N1B4_3304 0.14 -2.9 3,693,370 - Pf1N1B4_3304 0.79 -2.1 3,693,388 + Pf1N1B4_3304 0.81 -0.0 3,693,389 - Pf1N1B4_3304 0.81 -0.0 3,693,391 + Pf1N1B4_3304 0.82 -2.7 3,693,392 - -3.5 3,693,485 - -0.0 3,693,497 + -0.0 3,693,498 - -0.0 3,693,498 - -0.0 3,693,498 - -0.0 3,693,550 + -0.0 3,693,550 + -0.0 3,693,550 + -0.0 3,693,551 - -1.0 3,693,704 + Pf1N1B4_3305 0.21 -0.0 3,693,704 + Pf1N1B4_3305 0.21 -0.0 3,694,141 + Pf1N1B4_3305 0.75 -5.4 3,694,141 + Pf1N1B4_3305 0.75 -0.0 3,694,142 - Pf1N1B4_3305 0.75 -0.0 3,694,142 - Pf1N1B4_3305 0.75 -0.0 3,694,142 - -1.5 3,694,142 - Pf1N1B4_3305 0.75 -0.0 3,694,173 + Pf1N1B4_3305 0.79 -2.4 3,694,264 + Pf1N1B4_3305 0.90 -0.0 3,694,351 + -2.5 3,694,365 + -2.5 3,694,365 + -1.6 3,694,365 + -2.8 3,694,365 + -0.0 3,694,365 + -0.0 3,694,365 + -0.0 3,694,365 + -2.0 3,694,366 - -3.3 3,694,366 - -4.3 3,694,395 + -0.0 3,694,395 + -0.0 3,694,395 + -3.6 3,694,395 + -0.0 3,694,395 + -2.3 3,694,395 + -0.0 3,694,395 + -0.0 3,694,396 - -3.0 3,694,396 - -0.0 3,694,396 - -4.1 3,694,396 - -3.8 3,694,396 - -2.8
Or see this region's nucleotide sequence