Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3304

Experiment: Nitrate reduction, replicate C t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3302 and Pf1N1B4_3303 are separated by 11 nucleotidesPf1N1B4_3303 and Pf1N1B4_3304 are separated by 12 nucleotidesPf1N1B4_3304 and Pf1N1B4_3305 overlap by 4 nucleotidesPf1N1B4_3305 and Pf1N1B4_3306 are separated by 17 nucleotides Pf1N1B4_3302 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,691,355 to 3,691,996 _3302 Pf1N1B4_3303 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,692,008 to 3,692,733 _3303 Pf1N1B4_3304 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,692,746 to 3,693,534 _3304 Pf1N1B4_3305 - Urea carboxylase-related ABC transporter, permease protein, at 3,693,531 to 3,694,346 _3305 Pf1N1B4_3306 - Urea carboxylase-related ABC transporter, periplasmic substrate-binding protein, at 3,694,364 to 3,695,431 _3306 Position (kb) 3692 3693 3694Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3691.749 kb on + strand, within Pf1N1B4_3302at 3691.750 kb on - strand, within Pf1N1B4_3302at 3691.773 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strandat 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.813 kb on - strand, within Pf1N1B4_3302at 3691.840 kb on - strand, within Pf1N1B4_3302at 3692.195 kb on + strand, within Pf1N1B4_3303at 3692.207 kb on + strand, within Pf1N1B4_3303at 3692.208 kb on - strand, within Pf1N1B4_3303at 3692.208 kb on - strand, within Pf1N1B4_3303at 3692.208 kb on - strandat 3692.322 kb on + strand, within Pf1N1B4_3303at 3692.747 kb on + strandat 3692.837 kb on + strand, within Pf1N1B4_3304at 3692.837 kb on + strand, within Pf1N1B4_3304at 3692.838 kb on - strandat 3692.838 kb on - strand, within Pf1N1B4_3304at 3692.838 kb on - strand, within Pf1N1B4_3304at 3692.855 kb on + strandat 3692.856 kb on - strand, within Pf1N1B4_3304at 3692.856 kb on - strand, within Pf1N1B4_3304at 3693.370 kb on - strand, within Pf1N1B4_3304at 3693.388 kb on + strand, within Pf1N1B4_3304at 3693.389 kb on - strand, within Pf1N1B4_3304at 3693.391 kb on + strand, within Pf1N1B4_3304at 3693.392 kb on - strandat 3693.485 kb on - strandat 3693.497 kb on + strandat 3693.498 kb on - strandat 3693.498 kb on - strandat 3693.498 kb on - strandat 3693.550 kb on + strandat 3693.550 kb on + strandat 3693.550 kb on + strandat 3693.551 kb on - strandat 3693.704 kb on + strand, within Pf1N1B4_3305at 3693.704 kb on + strand, within Pf1N1B4_3305at 3694.141 kb on + strand, within Pf1N1B4_3305at 3694.141 kb on + strand, within Pf1N1B4_3305at 3694.142 kb on - strand, within Pf1N1B4_3305at 3694.142 kb on - strand, within Pf1N1B4_3305at 3694.142 kb on - strandat 3694.142 kb on - strand, within Pf1N1B4_3305at 3694.173 kb on + strand, within Pf1N1B4_3305at 3694.264 kb on + strand, within Pf1N1B4_3305at 3694.351 kb on + strandat 3694.365 kb on + strandat 3694.365 kb on + strandat 3694.365 kb on + strandat 3694.365 kb on + strandat 3694.365 kb on + strandat 3694.365 kb on + strandat 3694.365 kb on + strandat 3694.366 kb on - strandat 3694.366 kb on - strandat 3694.395 kb on + strandat 3694.395 kb on + strandat 3694.395 kb on + strandat 3694.395 kb on + strandat 3694.395 kb on + strandat 3694.395 kb on + strandat 3694.395 kb on + strandat 3694.396 kb on - strandat 3694.396 kb on - strandat 3694.396 kb on - strandat 3694.396 kb on - strandat 3694.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2
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3,691,749 + Pf1N1B4_3302 0.61 -1.5
3,691,750 - Pf1N1B4_3302 0.62 -0.0
3,691,773 + Pf1N1B4_3302 0.65 -4.7
3,691,805 + Pf1N1B4_3302 0.70 -2.5
3,691,805 + Pf1N1B4_3302 0.70 -1.7
3,691,805 + -0.0
3,691,805 + Pf1N1B4_3302 0.70 -0.0
3,691,805 + Pf1N1B4_3302 0.70 -2.4
3,691,806 - Pf1N1B4_3302 0.70 -0.0
3,691,806 - Pf1N1B4_3302 0.70 -0.0
3,691,806 - Pf1N1B4_3302 0.70 -0.0
3,691,806 - Pf1N1B4_3302 0.70 -1.7
3,691,813 - Pf1N1B4_3302 0.71 -0.0
3,691,840 - Pf1N1B4_3302 0.76 -1.0
3,692,195 + Pf1N1B4_3303 0.26 -0.0
3,692,207 + Pf1N1B4_3303 0.27 -0.0
3,692,208 - Pf1N1B4_3303 0.28 -0.8
3,692,208 - Pf1N1B4_3303 0.28 -2.1
3,692,208 - -0.0
3,692,322 + Pf1N1B4_3303 0.43 -3.5
3,692,747 + -0.0
3,692,837 + Pf1N1B4_3304 0.12 -0.0
3,692,837 + Pf1N1B4_3304 0.12 -2.9
3,692,838 - -4.0
3,692,838 - Pf1N1B4_3304 0.12 -0.0
3,692,838 - Pf1N1B4_3304 0.12 -2.6
3,692,855 + -4.1
3,692,856 - Pf1N1B4_3304 0.14 -2.7
3,692,856 - Pf1N1B4_3304 0.14 -2.9
3,693,370 - Pf1N1B4_3304 0.79 -2.1
3,693,388 + Pf1N1B4_3304 0.81 -0.0
3,693,389 - Pf1N1B4_3304 0.81 -0.0
3,693,391 + Pf1N1B4_3304 0.82 -2.7
3,693,392 - -3.5
3,693,485 - -0.0
3,693,497 + -0.0
3,693,498 - -0.0
3,693,498 - -0.0
3,693,498 - -0.0
3,693,550 + -0.0
3,693,550 + -0.0
3,693,550 + -0.0
3,693,551 - -1.0
3,693,704 + Pf1N1B4_3305 0.21 -0.0
3,693,704 + Pf1N1B4_3305 0.21 -0.0
3,694,141 + Pf1N1B4_3305 0.75 -5.4
3,694,141 + Pf1N1B4_3305 0.75 -0.0
3,694,142 - Pf1N1B4_3305 0.75 -0.0
3,694,142 - Pf1N1B4_3305 0.75 -0.0
3,694,142 - -1.5
3,694,142 - Pf1N1B4_3305 0.75 -0.0
3,694,173 + Pf1N1B4_3305 0.79 -2.4
3,694,264 + Pf1N1B4_3305 0.90 -0.0
3,694,351 + -2.5
3,694,365 + -2.5
3,694,365 + -1.6
3,694,365 + -2.8
3,694,365 + -0.0
3,694,365 + -0.0
3,694,365 + -0.0
3,694,365 + -2.0
3,694,366 - -3.3
3,694,366 - -4.3
3,694,395 + -0.0
3,694,395 + -0.0
3,694,395 + -3.6
3,694,395 + -0.0
3,694,395 + -2.3
3,694,395 + -0.0
3,694,395 + -0.0
3,694,396 - -3.0
3,694,396 - -0.0
3,694,396 - -4.1
3,694,396 - -3.8
3,694,396 - -2.8

Or see this region's nucleotide sequence