Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_31 and Pf1N1B4_32 are separated by 179 nucleotides Pf1N1B4_32 and Pf1N1B4_33 are separated by 174 nucleotides Pf1N1B4_33 and Pf1N1B4_34 are separated by 152 nucleotides
Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017
_31
Pf1N1B4_32 - Transcriptional regulator, TetR family, at 37,197 to 37,817
_32
Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422
_33
Pf1N1B4_34 - Xanthine permease, at 39,575 to 41,029
_34
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 37.017 kb on + strand at 37.267 kb on + strand at 37.465 kb on + strand, within Pf1N1B4_32 at 37.482 kb on - strand at 37.558 kb on - strand, within Pf1N1B4_32 at 37.608 kb on - strand at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.687 kb on - strand, within Pf1N1B4_32 at 37.933 kb on + strand at 37.934 kb on - strand at 38.084 kb on + strand at 38.141 kb on - strand, within Pf1N1B4_33 at 38.295 kb on + strand, within Pf1N1B4_33 at 38.316 kb on + strand, within Pf1N1B4_33 at 38.317 kb on - strand, within Pf1N1B4_33 at 38.317 kb on - strand, within Pf1N1B4_33 at 38.463 kb on + strand at 38.463 kb on + strand, within Pf1N1B4_33 at 38.464 kb on - strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.516 kb on - strand, within Pf1N1B4_33 at 38.516 kb on - strand, within Pf1N1B4_33 at 38.703 kb on + strand at 38.703 kb on + strand at 38.703 kb on + strand, within Pf1N1B4_33 at 38.703 kb on + strand, within Pf1N1B4_33 at 38.704 kb on - strand at 38.704 kb on - strand at 38.704 kb on - strand, within Pf1N1B4_33 at 38.706 kb on - strand, within Pf1N1B4_33 at 38.721 kb on - strand, within Pf1N1B4_33 at 38.863 kb on - strand, within Pf1N1B4_33 at 38.904 kb on - strand, within Pf1N1B4_33 at 38.968 kb on + strand, within Pf1N1B4_33 at 38.968 kb on + strand, within Pf1N1B4_33 at 38.969 kb on - strand at 39.156 kb on + strand, within Pf1N1B4_33 at 39.189 kb on + strand, within Pf1N1B4_33 at 39.548 kb on + strand at 39.691 kb on - strand at 39.883 kb on - strand, within Pf1N1B4_34
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 37,017 + -1.8 37,267 + -2.6 37,465 + Pf1N1B4_32 0.43 +0.1 37,482 - +0.1 37,558 - Pf1N1B4_32 0.58 +0.1 37,608 - +0.1 37,657 + Pf1N1B4_32 0.74 -2.5 37,657 + Pf1N1B4_32 0.74 +0.1 37,657 + Pf1N1B4_32 0.74 -3.9 37,658 - Pf1N1B4_32 0.74 +0.1 37,658 - -1.8 37,658 - -1.8 37,658 - -2.8 37,658 - Pf1N1B4_32 0.74 -5.0 37,658 - Pf1N1B4_32 0.74 -3.1 37,658 - Pf1N1B4_32 0.74 -1.7 37,658 - -1.3 37,687 - Pf1N1B4_32 0.79 +0.1 37,933 + -3.3 37,934 - -1.7 38,084 + -2.5 38,141 - Pf1N1B4_33 0.10 -2.5 38,295 + Pf1N1B4_33 0.21 -3.5 38,316 + Pf1N1B4_33 0.23 -2.5 38,317 - Pf1N1B4_33 0.23 -4.3 38,317 - Pf1N1B4_33 0.23 +0.1 38,463 + -1.5 38,463 + Pf1N1B4_33 0.33 +0.1 38,464 - Pf1N1B4_33 0.33 -3.8 38,515 + Pf1N1B4_33 0.37 -3.2 38,515 + Pf1N1B4_33 0.37 -2.6 38,515 + Pf1N1B4_33 0.37 -1.9 38,515 + Pf1N1B4_33 0.37 -3.7 38,516 - Pf1N1B4_33 0.37 -2.8 38,516 - Pf1N1B4_33 0.37 +0.1 38,703 + +0.1 38,703 + +0.1 38,703 + Pf1N1B4_33 0.50 +0.1 38,703 + Pf1N1B4_33 0.50 -1.9 38,704 - +0.1 38,704 - +0.1 38,704 - Pf1N1B4_33 0.50 -4.3 38,706 - Pf1N1B4_33 0.50 -3.5 38,721 - Pf1N1B4_33 0.51 +0.1 38,863 - Pf1N1B4_33 0.61 -4.1 38,904 - Pf1N1B4_33 0.64 -4.1 38,968 + Pf1N1B4_33 0.68 -0.9 38,968 + Pf1N1B4_33 0.68 +0.1 38,969 - +0.1 39,156 + Pf1N1B4_33 0.81 +0.1 39,189 + Pf1N1B4_33 0.84 -2.9 39,548 + -2.8 39,691 - -3.2 39,883 - Pf1N1B4_34 0.21 +0.1
Or see this region's nucleotide sequence