Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3288 and Pf1N1B4_3289 are separated by 110 nucleotides Pf1N1B4_3289 and Pf1N1B4_3290 are separated by 150 nucleotides Pf1N1B4_3290 and Pf1N1B4_3291 are separated by 13 nucleotides Pf1N1B4_3291 and Pf1N1B4_3292 are separated by 38 nucleotides
Pf1N1B4_3288 - Alternative cytochrome c oxidase polypeptide CoxN (EC 1.9.3.1), at 3,674,218 to 3,675,993
_3288
Pf1N1B4_3289 - Alternative cytochrome c oxidase polypeptide CoxO (EC 1.9.3.1), at 3,676,104 to 3,676,682
_3289
Pf1N1B4_3290 - Alternative cytochrome c oxidase polypeptide CoxP (EC 1.9.3.1), at 3,676,833 to 3,677,402
_3290
Pf1N1B4_3291 - Putative subunit of Alternative cytochrome c oxidase, at 3,677,416 to 3,677,733
_3291
Pf1N1B4_3292 - NAD/FAD-utilizing enzyme apparently involved in cell division, at 3,677,772 to 3,678,332
_3292
Position (kb)
3676
3677
3678 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3675.875 kb on - strand at 3675.875 kb on - strand at 3675.875 kb on - strand at 3675.875 kb on - strand at 3675.880 kb on + strand at 3675.881 kb on - strand at 3675.881 kb on - strand at 3675.897 kb on - strand at 3675.985 kb on - strand at 3676.240 kb on + strand, within Pf1N1B4_3289 at 3676.241 kb on - strand, within Pf1N1B4_3289 at 3676.241 kb on - strand, within Pf1N1B4_3289 at 3676.241 kb on - strand, within Pf1N1B4_3289 at 3676.326 kb on - strand, within Pf1N1B4_3289 at 3676.416 kb on + strand at 3676.417 kb on - strand, within Pf1N1B4_3289 at 3676.417 kb on - strand, within Pf1N1B4_3289 at 3676.452 kb on + strand, within Pf1N1B4_3289 at 3676.453 kb on - strand, within Pf1N1B4_3289 at 3676.510 kb on + strand, within Pf1N1B4_3289 at 3676.510 kb on + strand, within Pf1N1B4_3289 at 3676.510 kb on + strand, within Pf1N1B4_3289 at 3676.511 kb on - strand, within Pf1N1B4_3289 at 3676.511 kb on - strand, within Pf1N1B4_3289 at 3676.511 kb on - strand, within Pf1N1B4_3289 at 3676.520 kb on + strand, within Pf1N1B4_3289 at 3676.521 kb on - strand, within Pf1N1B4_3289 at 3676.564 kb on + strand, within Pf1N1B4_3289 at 3676.565 kb on - strand at 3676.565 kb on - strand, within Pf1N1B4_3289 at 3676.574 kb on + strand, within Pf1N1B4_3289 at 3676.581 kb on + strand, within Pf1N1B4_3289 at 3676.582 kb on - strand, within Pf1N1B4_3289 at 3676.582 kb on - strand, within Pf1N1B4_3289 at 3676.590 kb on + strand, within Pf1N1B4_3289 at 3676.590 kb on + strand, within Pf1N1B4_3289 at 3676.590 kb on + strand, within Pf1N1B4_3289 at 3676.591 kb on - strand, within Pf1N1B4_3289 at 3676.591 kb on - strand, within Pf1N1B4_3289 at 3676.594 kb on + strand, within Pf1N1B4_3289 at 3676.594 kb on + strand, within Pf1N1B4_3289 at 3676.594 kb on + strand, within Pf1N1B4_3289 at 3676.595 kb on - strand, within Pf1N1B4_3289 at 3676.595 kb on - strand, within Pf1N1B4_3289 at 3676.615 kb on + strand, within Pf1N1B4_3289 at 3676.647 kb on + strand at 3676.647 kb on + strand at 3676.648 kb on - strand at 3676.648 kb on - strand at 3676.648 kb on - strand at 3676.856 kb on + strand at 3676.857 kb on - strand at 3676.864 kb on + strand at 3676.865 kb on - strand at 3676.865 kb on - strand at 3676.865 kb on - strand at 3676.865 kb on - strand at 3676.887 kb on + strand at 3676.887 kb on + strand at 3676.888 kb on - strand at 3676.888 kb on - strand at 3676.976 kb on + strand, within Pf1N1B4_3290 at 3677.012 kb on + strand, within Pf1N1B4_3290 at 3677.259 kb on + strand, within Pf1N1B4_3290 at 3677.259 kb on + strand, within Pf1N1B4_3290 at 3677.284 kb on + strand, within Pf1N1B4_3290 at 3677.376 kb on - strand at 3677.376 kb on - strand at 3677.391 kb on + strand at 3677.391 kb on + strand at 3677.391 kb on + strand at 3677.395 kb on + strand at 3677.395 kb on + strand at 3677.395 kb on + strand at 3677.395 kb on + strand at 3677.395 kb on + strand at 3677.395 kb on + strand at 3677.396 kb on - strand at 3677.396 kb on - strand at 3677.396 kb on - strand at 3677.396 kb on - strand at 3677.401 kb on - strand at 3677.461 kb on - strand, within Pf1N1B4_3291 at 3677.512 kb on + strand at 3677.601 kb on + strand at 3677.637 kb on - strand, within Pf1N1B4_3291 at 3677.651 kb on + strand, within Pf1N1B4_3291 at 3677.695 kb on + strand, within Pf1N1B4_3291 at 3677.696 kb on - strand, within Pf1N1B4_3291 at 3677.696 kb on - strand, within Pf1N1B4_3291 at 3677.933 kb on + strand, within Pf1N1B4_3292 at 3677.934 kb on - strand at 3677.934 kb on - strand, within Pf1N1B4_3292 at 3677.949 kb on + strand, within Pf1N1B4_3292 at 3677.949 kb on + strand, within Pf1N1B4_3292 at 3677.949 kb on + strand, within Pf1N1B4_3292 at 3677.950 kb on - strand, within Pf1N1B4_3292 at 3677.950 kb on - strand, within Pf1N1B4_3292 at 3677.950 kb on - strand, within Pf1N1B4_3292 at 3677.950 kb on - strand, within Pf1N1B4_3292 at 3677.950 kb on - strand, within Pf1N1B4_3292 at 3677.950 kb on - strand, within Pf1N1B4_3292 at 3678.030 kb on + strand, within Pf1N1B4_3292 at 3678.031 kb on - strand, within Pf1N1B4_3292 at 3678.031 kb on - strand, within Pf1N1B4_3292 at 3678.031 kb on - strand, within Pf1N1B4_3292 at 3678.031 kb on - strand, within Pf1N1B4_3292 at 3678.033 kb on - strand, within Pf1N1B4_3292 at 3678.050 kb on + strand, within Pf1N1B4_3292 at 3678.051 kb on - strand, within Pf1N1B4_3292 at 3678.051 kb on - strand, within Pf1N1B4_3292 at 3678.094 kb on + strand, within Pf1N1B4_3292 at 3678.315 kb on - strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.316 kb on + strand at 3678.317 kb on - strand at 3678.317 kb on - strand at 3678.319 kb on + strand at 3678.319 kb on + strand at 3678.360 kb on + strand at 3678.361 kb on - strand at 3678.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 3,675,875 - -1.9 3,675,875 - -0.0 3,675,875 - -0.0 3,675,875 - -0.0 3,675,880 + -1.9 3,675,881 - -1.5 3,675,881 - -1.5 3,675,897 - -0.0 3,675,985 - -2.2 3,676,240 + Pf1N1B4_3289 0.23 -1.3 3,676,241 - Pf1N1B4_3289 0.24 -0.0 3,676,241 - Pf1N1B4_3289 0.24 -1.4 3,676,241 - Pf1N1B4_3289 0.24 -0.0 3,676,326 - Pf1N1B4_3289 0.38 -0.0 3,676,416 + -0.0 3,676,417 - Pf1N1B4_3289 0.54 -2.1 3,676,417 - Pf1N1B4_3289 0.54 -1.8 3,676,452 + Pf1N1B4_3289 0.60 -0.0 3,676,453 - Pf1N1B4_3289 0.60 -0.9 3,676,510 + Pf1N1B4_3289 0.70 -2.5 3,676,510 + Pf1N1B4_3289 0.70 -0.0 3,676,510 + Pf1N1B4_3289 0.70 -2.2 3,676,511 - Pf1N1B4_3289 0.70 -0.0 3,676,511 - Pf1N1B4_3289 0.70 -0.0 3,676,511 - Pf1N1B4_3289 0.70 -2.8 3,676,520 + Pf1N1B4_3289 0.72 -0.0 3,676,521 - Pf1N1B4_3289 0.72 -0.0 3,676,564 + Pf1N1B4_3289 0.79 -0.0 3,676,565 - -0.0 3,676,565 - Pf1N1B4_3289 0.80 -2.4 3,676,574 + Pf1N1B4_3289 0.81 -1.2 3,676,581 + Pf1N1B4_3289 0.82 -0.0 3,676,582 - Pf1N1B4_3289 0.83 -2.7 3,676,582 - Pf1N1B4_3289 0.83 -2.9 3,676,590 + Pf1N1B4_3289 0.84 -2.8 3,676,590 + Pf1N1B4_3289 0.84 -2.5 3,676,590 + Pf1N1B4_3289 0.84 -0.0 3,676,591 - Pf1N1B4_3289 0.84 -0.0 3,676,591 - Pf1N1B4_3289 0.84 -0.0 3,676,594 + Pf1N1B4_3289 0.85 -0.0 3,676,594 + Pf1N1B4_3289 0.85 -0.0 3,676,594 + Pf1N1B4_3289 0.85 -0.0 3,676,595 - Pf1N1B4_3289 0.85 -2.3 3,676,595 - Pf1N1B4_3289 0.85 -3.6 3,676,615 + Pf1N1B4_3289 0.88 -0.0 3,676,647 + -0.0 3,676,647 + -0.0 3,676,648 - -4.6 3,676,648 - -2.6 3,676,648 - -0.0 3,676,856 + -0.8 3,676,857 - -1.9 3,676,864 + -0.0 3,676,865 - +1.0 3,676,865 - -3.1 3,676,865 - -2.2 3,676,865 - -0.0 3,676,887 + -0.0 3,676,887 + -0.0 3,676,888 - -0.0 3,676,888 - -0.0 3,676,976 + Pf1N1B4_3290 0.25 -0.0 3,677,012 + Pf1N1B4_3290 0.31 -0.0 3,677,259 + Pf1N1B4_3290 0.75 -0.0 3,677,259 + Pf1N1B4_3290 0.75 -0.0 3,677,284 + Pf1N1B4_3290 0.79 -0.0 3,677,376 - -0.0 3,677,376 - -0.0 3,677,391 + -0.0 3,677,391 + -3.4 3,677,391 + -2.7 3,677,395 + -0.0 3,677,395 + -0.0 3,677,395 + -1.0 3,677,395 + -0.0 3,677,395 + -0.0 3,677,395 + -3.8 3,677,396 - -0.0 3,677,396 - -2.3 3,677,396 - -4.3 3,677,396 - -3.2 3,677,401 - -0.0 3,677,461 - Pf1N1B4_3291 0.14 -0.0 3,677,512 + -0.0 3,677,601 + -2.5 3,677,637 - Pf1N1B4_3291 0.69 -1.8 3,677,651 + Pf1N1B4_3291 0.74 -2.7 3,677,695 + Pf1N1B4_3291 0.88 -0.0 3,677,696 - Pf1N1B4_3291 0.88 -4.5 3,677,696 - Pf1N1B4_3291 0.88 -3.2 3,677,933 + Pf1N1B4_3292 0.29 -2.7 3,677,934 - -0.0 3,677,934 - Pf1N1B4_3292 0.29 -0.0 3,677,949 + Pf1N1B4_3292 0.32 -0.0 3,677,949 + Pf1N1B4_3292 0.32 -0.0 3,677,949 + Pf1N1B4_3292 0.32 -3.0 3,677,950 - Pf1N1B4_3292 0.32 -0.0 3,677,950 - Pf1N1B4_3292 0.32 -0.0 3,677,950 - Pf1N1B4_3292 0.32 -0.0 3,677,950 - Pf1N1B4_3292 0.32 -0.0 3,677,950 - Pf1N1B4_3292 0.32 -0.0 3,677,950 - Pf1N1B4_3292 0.32 -0.0 3,678,030 + Pf1N1B4_3292 0.46 -0.0 3,678,031 - Pf1N1B4_3292 0.46 -2.0 3,678,031 - Pf1N1B4_3292 0.46 -0.0 3,678,031 - Pf1N1B4_3292 0.46 -0.0 3,678,031 - Pf1N1B4_3292 0.46 -4.2 3,678,033 - Pf1N1B4_3292 0.47 -0.0 3,678,050 + Pf1N1B4_3292 0.50 -2.7 3,678,051 - Pf1N1B4_3292 0.50 -0.0 3,678,051 - Pf1N1B4_3292 0.50 -2.2 3,678,094 + Pf1N1B4_3292 0.57 -0.0 3,678,315 - -1.7 3,678,316 + -0.0 3,678,316 + -0.0 3,678,316 + -2.5 3,678,316 + -0.0 3,678,316 + -1.6 3,678,316 + -2.3 3,678,316 + -0.0 3,678,316 + -0.0 3,678,316 + -0.0 3,678,316 + -0.0 3,678,317 - -4.1 3,678,317 - -0.0 3,678,319 + -0.0 3,678,319 + -0.0 3,678,360 + -2.2 3,678,361 - -0.0 3,678,361 - -2.9
Or see this region's nucleotide sequence