Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2737 and Pf1N1B4_2738 are separated by 244 nucleotides Pf1N1B4_2738 and Pf1N1B4_2739 are separated by 90 nucleotides Pf1N1B4_2739 and Pf1N1B4_2740 are separated by 105 nucleotides
Pf1N1B4_2737 - NAD(FAD)-utilizing dehydrogenases, at 3,075,733 to 3,077,010
_2737
Pf1N1B4_2738 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 3,077,255 to 3,078,175
_2738
Pf1N1B4_2739 - acetyltransferase, GNAT family, at 3,078,266 to 3,078,835
_2739
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Position (kb)
3077
3078
3079 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3076.399 kb on + strand, within Pf1N1B4_2737 at 3076.400 kb on - strand, within Pf1N1B4_2737 at 3076.400 kb on - strand, within Pf1N1B4_2737 at 3076.400 kb on - strand, within Pf1N1B4_2737 at 3076.576 kb on - strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.579 kb on + strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.580 kb on - strand, within Pf1N1B4_2737 at 3076.729 kb on + strand, within Pf1N1B4_2737 at 3076.763 kb on + strand, within Pf1N1B4_2737 at 3076.763 kb on + strand, within Pf1N1B4_2737 at 3076.764 kb on - strand, within Pf1N1B4_2737 at 3076.764 kb on - strand, within Pf1N1B4_2737 at 3076.862 kb on + strand at 3076.863 kb on - strand, within Pf1N1B4_2737 at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.013 kb on + strand at 3077.014 kb on - strand at 3077.086 kb on + strand at 3077.086 kb on + strand at 3077.087 kb on - strand at 3077.087 kb on - strand at 3077.173 kb on + strand at 3077.174 kb on - strand at 3077.174 kb on - strand at 3077.252 kb on - strand at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.487 kb on + strand, within Pf1N1B4_2738 at 3077.488 kb on - strand, within Pf1N1B4_2738 at 3077.488 kb on - strand, within Pf1N1B4_2738 at 3077.509 kb on - strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.523 kb on + strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.524 kb on - strand, within Pf1N1B4_2738 at 3077.553 kb on + strand, within Pf1N1B4_2738 at 3077.553 kb on + strand, within Pf1N1B4_2738 at 3077.554 kb on - strand, within Pf1N1B4_2738 at 3077.751 kb on + strand, within Pf1N1B4_2738 at 3077.751 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.805 kb on + strand, within Pf1N1B4_2738 at 3077.806 kb on - strand, within Pf1N1B4_2738 at 3077.806 kb on - strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.976 kb on + strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.977 kb on - strand, within Pf1N1B4_2738 at 3077.987 kb on - strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.988 kb on + strand, within Pf1N1B4_2738 at 3077.989 kb on - strand, within Pf1N1B4_2738 at 3077.989 kb on - strand, within Pf1N1B4_2738 at 3078.144 kb on + strand at 3078.145 kb on - strand at 3078.145 kb on - strand at 3078.156 kb on + strand at 3078.156 kb on + strand at 3078.157 kb on - strand at 3078.157 kb on - strand at 3078.157 kb on - strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.184 kb on + strand at 3078.185 kb on - strand at 3078.185 kb on - strand at 3078.185 kb on - strand at 3078.186 kb on + strand at 3078.186 kb on + strand at 3078.186 kb on + strand at 3078.200 kb on + strand at 3078.200 kb on + strand at 3078.200 kb on + strand at 3078.220 kb on + strand at 3078.220 kb on + strand at 3078.221 kb on - strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.231 kb on + strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.232 kb on - strand at 3078.538 kb on + strand, within Pf1N1B4_2739 at 3078.539 kb on - strand, within Pf1N1B4_2739 at 3078.593 kb on + strand, within Pf1N1B4_2739 at 3078.791 kb on + strand at 3078.792 kb on - strand at 3078.792 kb on - strand at 3078.833 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.851 kb on + strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.852 kb on - strand at 3078.879 kb on + strand at 3079.057 kb on - strand, within Pf1N1B4_2740 at 3079.127 kb on + strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.128 kb on - strand, within Pf1N1B4_2740 at 3079.166 kb on + strand, within Pf1N1B4_2740
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 3,076,399 + Pf1N1B4_2737 0.52 -3.8 3,076,400 - Pf1N1B4_2737 0.52 -2.5 3,076,400 - Pf1N1B4_2737 0.52 -0.0 3,076,400 - Pf1N1B4_2737 0.52 -0.0 3,076,576 - Pf1N1B4_2737 0.66 -3.4 3,076,579 + Pf1N1B4_2737 0.66 +1.0 3,076,579 + Pf1N1B4_2737 0.66 -1.3 3,076,579 + Pf1N1B4_2737 0.66 -1.9 3,076,579 + Pf1N1B4_2737 0.66 -0.0 3,076,579 + Pf1N1B4_2737 0.66 -0.0 3,076,579 + Pf1N1B4_2737 0.66 -2.4 3,076,579 + Pf1N1B4_2737 0.66 -0.0 3,076,580 - Pf1N1B4_2737 0.66 -1.9 3,076,580 - Pf1N1B4_2737 0.66 -0.0 3,076,580 - Pf1N1B4_2737 0.66 -0.0 3,076,580 - Pf1N1B4_2737 0.66 -0.0 3,076,729 + Pf1N1B4_2737 0.78 -0.0 3,076,763 + Pf1N1B4_2737 0.81 -0.0 3,076,763 + Pf1N1B4_2737 0.81 -0.0 3,076,764 - Pf1N1B4_2737 0.81 -2.6 3,076,764 - Pf1N1B4_2737 0.81 -0.0 3,076,862 + -0.0 3,076,863 - Pf1N1B4_2737 0.88 -1.7 3,077,013 + -3.1 3,077,013 + -0.0 3,077,013 + -0.0 3,077,013 + -0.0 3,077,013 + -2.6 3,077,014 - -0.0 3,077,086 + -0.0 3,077,086 + -2.9 3,077,087 - -0.0 3,077,087 - -1.8 3,077,173 + -3.2 3,077,174 - -0.0 3,077,174 - -0.0 3,077,252 - -2.5 3,077,487 + Pf1N1B4_2738 0.25 -3.0 3,077,487 + Pf1N1B4_2738 0.25 -0.0 3,077,487 + Pf1N1B4_2738 0.25 -0.7 3,077,488 - Pf1N1B4_2738 0.25 -0.0 3,077,488 - Pf1N1B4_2738 0.25 -2.9 3,077,509 - Pf1N1B4_2738 0.28 -0.0 3,077,523 + Pf1N1B4_2738 0.29 -0.0 3,077,523 + Pf1N1B4_2738 0.29 -2.1 3,077,523 + Pf1N1B4_2738 0.29 -0.0 3,077,524 - Pf1N1B4_2738 0.29 -0.0 3,077,524 - Pf1N1B4_2738 0.29 -3.3 3,077,524 - Pf1N1B4_2738 0.29 -1.8 3,077,553 + Pf1N1B4_2738 0.32 -0.0 3,077,553 + Pf1N1B4_2738 0.32 -0.0 3,077,554 - Pf1N1B4_2738 0.32 -0.0 3,077,751 + Pf1N1B4_2738 0.54 -0.0 3,077,751 + Pf1N1B4_2738 0.54 -0.0 3,077,805 + Pf1N1B4_2738 0.60 -0.0 3,077,805 + Pf1N1B4_2738 0.60 -1.0 3,077,805 + Pf1N1B4_2738 0.60 -3.3 3,077,806 - Pf1N1B4_2738 0.60 -0.0 3,077,806 - Pf1N1B4_2738 0.60 -0.0 3,077,976 + Pf1N1B4_2738 0.78 -3.0 3,077,976 + Pf1N1B4_2738 0.78 -0.0 3,077,976 + Pf1N1B4_2738 0.78 -2.7 3,077,976 + Pf1N1B4_2738 0.78 -2.2 3,077,976 + Pf1N1B4_2738 0.78 -0.0 3,077,977 - Pf1N1B4_2738 0.78 -0.0 3,077,977 - Pf1N1B4_2738 0.78 -0.0 3,077,977 - Pf1N1B4_2738 0.78 -0.0 3,077,977 - Pf1N1B4_2738 0.78 -0.0 3,077,987 - Pf1N1B4_2738 0.79 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -3.3 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + -1.6 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -3.1 3,077,988 + Pf1N1B4_2738 0.80 -3.2 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -4.3 3,077,988 + Pf1N1B4_2738 0.80 -0.0 3,077,988 + Pf1N1B4_2738 0.80 -3.9 3,077,988 + Pf1N1B4_2738 0.80 -3.1 3,077,989 - Pf1N1B4_2738 0.80 -2.1 3,077,989 - Pf1N1B4_2738 0.80 -0.0 3,078,144 + -0.0 3,078,145 - -1.8 3,078,145 - -2.0 3,078,156 + -0.0 3,078,156 + -0.0 3,078,157 - -0.0 3,078,157 - -2.5 3,078,157 - -0.0 3,078,184 + -0.0 3,078,184 + -0.0 3,078,184 + -0.0 3,078,184 + -3.9 3,078,185 - -0.4 3,078,185 - -1.6 3,078,185 - -0.0 3,078,186 + -2.4 3,078,186 + -0.0 3,078,186 + -0.0 3,078,200 + -0.0 3,078,200 + -2.9 3,078,200 + -0.0 3,078,220 + -0.0 3,078,220 + -0.0 3,078,221 - -0.0 3,078,231 + -0.0 3,078,231 + -1.7 3,078,231 + -0.0 3,078,231 + -0.0 3,078,231 + -0.0 3,078,232 - -2.8 3,078,232 - -2.1 3,078,232 - -2.1 3,078,232 - -2.9 3,078,538 + Pf1N1B4_2739 0.48 -0.0 3,078,539 - Pf1N1B4_2739 0.48 -2.4 3,078,593 + Pf1N1B4_2739 0.57 -2.6 3,078,791 + -3.4 3,078,792 - -0.0 3,078,792 - -0.0 3,078,833 + -2.0 3,078,851 + -2.2 3,078,851 + -0.0 3,078,851 + -0.0 3,078,851 + -0.0 3,078,852 - -1.7 3,078,852 - -2.6 3,078,852 - -0.0 3,078,852 - -3.6 3,078,852 - -0.0 3,078,879 + -0.0 3,079,057 - Pf1N1B4_2740 0.13 -2.4 3,079,127 + Pf1N1B4_2740 0.21 -2.8 3,079,128 - Pf1N1B4_2740 0.21 -0.0 3,079,128 - Pf1N1B4_2740 0.21 -2.4 3,079,166 + Pf1N1B4_2740 0.26 -1.4
Or see this region's nucleotide sequence