Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2405 and Pf1N1B4_2406 are separated by 45 nucleotides Pf1N1B4_2406 and Pf1N1B4_2407 are separated by 100 nucleotides Pf1N1B4_2407 and Pf1N1B4_2408 are separated by 123 nucleotides
Pf1N1B4_2405 - Sensory box/GGDEF family protein, at 2,723,860 to 2,725,965
_2405
Pf1N1B4_2406 - COG1451: Predicted metal-dependent hydrolase, at 2,726,011 to 2,726,502
_2406
Pf1N1B4_2407 - FIG00953367: hypothetical protein, at 2,726,603 to 2,726,899
_2407
Pf1N1B4_2408 - ElaA protein, at 2,727,023 to 2,727,475
_2408
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2725.068 kb on + strand, within Pf1N1B4_2405 at 2725.108 kb on + strand, within Pf1N1B4_2405 at 2725.109 kb on - strand, within Pf1N1B4_2405 at 2725.109 kb on - strand, within Pf1N1B4_2405 at 2725.147 kb on + strand, within Pf1N1B4_2405 at 2725.147 kb on + strand, within Pf1N1B4_2405 at 2725.148 kb on - strand, within Pf1N1B4_2405 at 2725.240 kb on + strand, within Pf1N1B4_2405 at 2725.240 kb on + strand, within Pf1N1B4_2405 at 2725.240 kb on + strand, within Pf1N1B4_2405 at 2725.241 kb on - strand, within Pf1N1B4_2405 at 2725.241 kb on - strand, within Pf1N1B4_2405 at 2725.241 kb on - strand, within Pf1N1B4_2405 at 2725.241 kb on - strand, within Pf1N1B4_2405 at 2725.393 kb on + strand, within Pf1N1B4_2405 at 2725.393 kb on + strand, within Pf1N1B4_2405 at 2725.393 kb on + strand, within Pf1N1B4_2405 at 2725.551 kb on - strand, within Pf1N1B4_2405 at 2725.585 kb on - strand, within Pf1N1B4_2405 at 2725.808 kb on + strand at 2725.808 kb on + strand at 2725.808 kb on + strand at 2725.843 kb on + strand at 2725.873 kb on + strand at 2725.873 kb on + strand at 2725.873 kb on + strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.973 kb on - strand at 2725.973 kb on - strand at 2726.026 kb on + strand at 2726.026 kb on + strand at 2726.026 kb on + strand at 2726.026 kb on + strand at 2726.047 kb on - strand at 2726.047 kb on - strand at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.152 kb on + strand at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.153 kb on - strand, within Pf1N1B4_2406 at 2726.153 kb on - strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.182 kb on + strand, within Pf1N1B4_2406 at 2726.183 kb on - strand, within Pf1N1B4_2406 at 2726.416 kb on + strand, within Pf1N1B4_2406 at 2726.416 kb on + strand at 2726.416 kb on + strand, within Pf1N1B4_2406 at 2726.416 kb on + strand, within Pf1N1B4_2406 at 2726.417 kb on - strand, within Pf1N1B4_2406 at 2726.468 kb on + strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.476 kb on + strand at 2726.476 kb on + strand at 2726.476 kb on + strand at 2726.476 kb on + strand at 2726.477 kb on - strand at 2726.493 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.501 kb on - strand at 2726.501 kb on - strand at 2726.501 kb on - strand at 2726.501 kb on - strand at 2726.586 kb on + strand at 2726.586 kb on + strand at 2726.586 kb on + strand at 2726.587 kb on - strand at 2726.589 kb on + strand at 2726.589 kb on + strand at 2726.589 kb on + strand at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.646 kb on - strand, within Pf1N1B4_2407 at 2726.646 kb on - strand, within Pf1N1B4_2407 at 2726.655 kb on - strand, within Pf1N1B4_2407 at 2726.860 kb on + strand, within Pf1N1B4_2407 at 2726.860 kb on + strand, within Pf1N1B4_2407 at 2726.860 kb on + strand, within Pf1N1B4_2407 at 2726.861 kb on - strand, within Pf1N1B4_2407 at 2726.875 kb on + strand at 2726.884 kb on - strand at 2726.902 kb on - strand at 2727.024 kb on + strand at 2727.066 kb on + strand at 2727.103 kb on - strand, within Pf1N1B4_2408 at 2727.103 kb on - strand, within Pf1N1B4_2408 at 2727.106 kb on - strand, within Pf1N1B4_2408 at 2727.106 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.188 kb on + strand, within Pf1N1B4_2408 at 2727.188 kb on + strand, within Pf1N1B4_2408 at 2727.189 kb on - strand, within Pf1N1B4_2408 at 2727.189 kb on - strand, within Pf1N1B4_2408 at 2727.189 kb on - strand, within Pf1N1B4_2408 at 2727.264 kb on - strand, within Pf1N1B4_2408 at 2727.264 kb on - strand, within Pf1N1B4_2408 at 2727.288 kb on + strand at 2727.288 kb on + strand, within Pf1N1B4_2408 at 2727.289 kb on - strand, within Pf1N1B4_2408 at 2727.289 kb on - strand, within Pf1N1B4_2408 at 2727.367 kb on - strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.421 kb on - strand, within Pf1N1B4_2408 at 2727.421 kb on - strand, within Pf1N1B4_2408 at 2727.421 kb on - strand, within Pf1N1B4_2408
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 2,725,068 + Pf1N1B4_2405 0.57 -2.4 2,725,108 + Pf1N1B4_2405 0.59 +0.0 2,725,109 - Pf1N1B4_2405 0.59 -2.8 2,725,109 - Pf1N1B4_2405 0.59 +0.0 2,725,147 + Pf1N1B4_2405 0.61 +0.0 2,725,147 + Pf1N1B4_2405 0.61 +0.0 2,725,148 - Pf1N1B4_2405 0.61 +0.0 2,725,240 + Pf1N1B4_2405 0.66 -3.0 2,725,240 + Pf1N1B4_2405 0.66 +0.0 2,725,240 + Pf1N1B4_2405 0.66 +0.0 2,725,241 - Pf1N1B4_2405 0.66 -2.6 2,725,241 - Pf1N1B4_2405 0.66 +0.0 2,725,241 - Pf1N1B4_2405 0.66 +0.0 2,725,241 - Pf1N1B4_2405 0.66 +0.0 2,725,393 + Pf1N1B4_2405 0.73 -3.6 2,725,393 + Pf1N1B4_2405 0.73 +0.0 2,725,393 + Pf1N1B4_2405 0.73 -3.4 2,725,551 - Pf1N1B4_2405 0.80 +0.0 2,725,585 - Pf1N1B4_2405 0.82 -2.7 2,725,808 + -2.7 2,725,808 + +0.0 2,725,808 + +0.0 2,725,843 + -2.8 2,725,873 + +0.0 2,725,873 + +0.0 2,725,873 + -1.9 2,725,874 - +0.0 2,725,874 - -3.5 2,725,874 - +0.0 2,725,874 - +0.0 2,725,874 - -2.2 2,725,973 - -2.5 2,725,973 - -2.2 2,726,026 + +0.0 2,726,026 + +0.0 2,726,026 + +0.0 2,726,026 + -1.7 2,726,047 - -2.3 2,726,047 - -2.1 2,726,152 + Pf1N1B4_2406 0.29 -1.8 2,726,152 + Pf1N1B4_2406 0.29 +0.0 2,726,152 + Pf1N1B4_2406 0.29 +0.0 2,726,152 + +0.0 2,726,152 + Pf1N1B4_2406 0.29 +0.0 2,726,153 - Pf1N1B4_2406 0.29 +0.0 2,726,153 - Pf1N1B4_2406 0.29 -0.4 2,726,158 + Pf1N1B4_2406 0.30 +0.0 2,726,158 + Pf1N1B4_2406 0.30 +0.0 2,726,158 + Pf1N1B4_2406 0.30 +0.1 2,726,158 + Pf1N1B4_2406 0.30 +0.0 2,726,158 + Pf1N1B4_2406 0.30 +0.0 2,726,182 + Pf1N1B4_2406 0.35 +0.0 2,726,183 - Pf1N1B4_2406 0.35 +0.0 2,726,416 + Pf1N1B4_2406 0.82 -1.3 2,726,416 + -3.5 2,726,416 + Pf1N1B4_2406 0.82 -1.0 2,726,416 + Pf1N1B4_2406 0.82 +0.0 2,726,417 - Pf1N1B4_2406 0.83 +0.0 2,726,468 + -3.4 2,726,469 - -3.6 2,726,469 - +0.0 2,726,469 - +0.0 2,726,469 - -0.8 2,726,469 - +1.0 2,726,469 - -3.1 2,726,469 - -2.0 2,726,476 + +0.0 2,726,476 + +0.0 2,726,476 + -2.6 2,726,476 + -2.2 2,726,477 - +0.0 2,726,493 + -2.9 2,726,500 + -1.9 2,726,500 + +0.0 2,726,500 + +0.0 2,726,500 + +0.0 2,726,500 + +0.0 2,726,500 + -1.8 2,726,500 + -2.3 2,726,500 + +0.0 2,726,500 + -0.5 2,726,500 + +0.0 2,726,501 - -2.0 2,726,501 - -2.8 2,726,501 - -3.6 2,726,501 - -2.3 2,726,586 + +0.0 2,726,586 + +0.0 2,726,586 + +0.0 2,726,587 - +0.0 2,726,589 + +0.0 2,726,589 + -1.8 2,726,589 + +0.0 2,726,645 + Pf1N1B4_2407 0.14 -1.0 2,726,645 + Pf1N1B4_2407 0.14 +0.0 2,726,645 + Pf1N1B4_2407 0.14 +0.0 2,726,645 + Pf1N1B4_2407 0.14 -2.3 2,726,645 + Pf1N1B4_2407 0.14 -3.6 2,726,646 - Pf1N1B4_2407 0.14 +0.0 2,726,646 - Pf1N1B4_2407 0.14 +0.0 2,726,655 - Pf1N1B4_2407 0.18 +0.0 2,726,860 + Pf1N1B4_2407 0.87 -2.4 2,726,860 + Pf1N1B4_2407 0.87 +0.0 2,726,860 + Pf1N1B4_2407 0.87 -4.0 2,726,861 - Pf1N1B4_2407 0.87 +0.0 2,726,875 + +0.0 2,726,884 - +0.0 2,726,902 - +0.0 2,727,024 + +0.0 2,727,066 + +0.0 2,727,103 - Pf1N1B4_2408 0.18 -2.5 2,727,103 - Pf1N1B4_2408 0.18 +0.0 2,727,106 - Pf1N1B4_2408 0.18 +0.0 2,727,106 - Pf1N1B4_2408 0.18 -2.3 2,727,136 - Pf1N1B4_2408 0.25 +0.0 2,727,136 - Pf1N1B4_2408 0.25 +0.0 2,727,136 - Pf1N1B4_2408 0.25 +0.0 2,727,136 - Pf1N1B4_2408 0.25 -2.1 2,727,136 - Pf1N1B4_2408 0.25 +0.0 2,727,136 - Pf1N1B4_2408 0.25 +0.0 2,727,188 + Pf1N1B4_2408 0.36 -2.0 2,727,188 + Pf1N1B4_2408 0.36 -3.8 2,727,189 - Pf1N1B4_2408 0.37 -3.6 2,727,189 - Pf1N1B4_2408 0.37 -2.0 2,727,189 - Pf1N1B4_2408 0.37 -2.8 2,727,264 - Pf1N1B4_2408 0.53 +0.0 2,727,264 - Pf1N1B4_2408 0.53 +0.0 2,727,288 + -1.8 2,727,288 + Pf1N1B4_2408 0.58 -1.9 2,727,289 - Pf1N1B4_2408 0.59 -2.7 2,727,289 - Pf1N1B4_2408 0.59 -1.6 2,727,367 - Pf1N1B4_2408 0.76 -2.6 2,727,420 + Pf1N1B4_2408 0.88 +0.0 2,727,420 + Pf1N1B4_2408 0.88 +0.0 2,727,420 + Pf1N1B4_2408 0.88 +0.0 2,727,420 + Pf1N1B4_2408 0.88 -1.2 2,727,420 + Pf1N1B4_2408 0.88 -1.9 2,727,421 - Pf1N1B4_2408 0.88 +0.0 2,727,421 - Pf1N1B4_2408 0.88 +0.0 2,727,421 - Pf1N1B4_2408 0.88 +0.0
Or see this region's nucleotide sequence