Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1883 and Pf1N1B4_1884 are separated by 12 nucleotides Pf1N1B4_1884 and Pf1N1B4_1885 are separated by 10 nucleotides
Pf1N1B4_1883 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 2,153,574 to 2,155,190
_1883
Pf1N1B4_1884 - Sensory box histidine kinase, at 2,155,203 to 2,156,993
_1884
Pf1N1B4_1885 - alginate biosynthesis transcriptional regulatory protein AlgB, at 2,157,004 to 2,158,350
_1885
Position (kb)
2155
2156
2157 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2154.244 kb on - strand, within Pf1N1B4_1883 at 2154.271 kb on - strand, within Pf1N1B4_1883 at 2154.271 kb on - strand, within Pf1N1B4_1883 at 2154.271 kb on - strand, within Pf1N1B4_1883 at 2154.310 kb on + strand, within Pf1N1B4_1883 at 2154.310 kb on + strand, within Pf1N1B4_1883 at 2154.311 kb on - strand, within Pf1N1B4_1883 at 2154.311 kb on - strand, within Pf1N1B4_1883 at 2154.311 kb on - strand, within Pf1N1B4_1883 at 2154.319 kb on + strand, within Pf1N1B4_1883 at 2154.320 kb on - strand, within Pf1N1B4_1883 at 2154.364 kb on + strand, within Pf1N1B4_1883 at 2154.365 kb on - strand, within Pf1N1B4_1883 at 2154.388 kb on + strand, within Pf1N1B4_1883 at 2154.388 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.445 kb on + strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.446 kb on - strand, within Pf1N1B4_1883 at 2154.456 kb on + strand, within Pf1N1B4_1883 at 2154.456 kb on + strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.457 kb on - strand, within Pf1N1B4_1883 at 2154.557 kb on + strand, within Pf1N1B4_1883 at 2154.575 kb on + strand at 2154.610 kb on + strand, within Pf1N1B4_1883 at 2154.611 kb on - strand, within Pf1N1B4_1883 at 2154.611 kb on - strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.663 kb on + strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.664 kb on - strand, within Pf1N1B4_1883 at 2154.876 kb on + strand, within Pf1N1B4_1883 at 2154.877 kb on - strand, within Pf1N1B4_1883 at 2154.891 kb on + strand, within Pf1N1B4_1883 at 2154.939 kb on - strand, within Pf1N1B4_1883 at 2155.164 kb on - strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.204 kb on + strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.205 kb on - strand at 2155.206 kb on + strand at 2155.222 kb on + strand at 2155.222 kb on + strand at 2155.222 kb on + strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.223 kb on - strand at 2155.252 kb on + strand at 2155.253 kb on - strand at 2155.253 kb on - strand at 2155.375 kb on + strand at 2155.409 kb on + strand, within Pf1N1B4_1884 at 2155.410 kb on - strand at 2155.410 kb on - strand, within Pf1N1B4_1884 at 2155.446 kb on + strand, within Pf1N1B4_1884 at 2155.447 kb on - strand, within Pf1N1B4_1884 at 2155.447 kb on - strand, within Pf1N1B4_1884 at 2155.926 kb on - strand, within Pf1N1B4_1884 at 2155.959 kb on - strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.960 kb on + strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2155.961 kb on - strand, within Pf1N1B4_1884 at 2156.035 kb on - strand, within Pf1N1B4_1884 at 2156.037 kb on + strand, within Pf1N1B4_1884 at 2156.037 kb on + strand, within Pf1N1B4_1884 at 2156.038 kb on - strand, within Pf1N1B4_1884 at 2156.250 kb on - strand, within Pf1N1B4_1884 at 2156.348 kb on + strand at 2156.607 kb on + strand, within Pf1N1B4_1884 at 2156.608 kb on - strand, within Pf1N1B4_1884 at 2156.646 kb on + strand, within Pf1N1B4_1884 at 2156.646 kb on + strand, within Pf1N1B4_1884 at 2156.647 kb on - strand, within Pf1N1B4_1884 at 2156.647 kb on - strand, within Pf1N1B4_1884 at 2156.684 kb on - strand, within Pf1N1B4_1884 at 2156.936 kb on + strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.937 kb on - strand at 2156.952 kb on - strand at 2157.014 kb on + strand at 2157.014 kb on + strand at 2157.014 kb on + strand at 2157.015 kb on - strand at 2157.015 kb on - strand at 2157.015 kb on - strand at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.222 kb on + strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.223 kb on - strand, within Pf1N1B4_1885 at 2157.279 kb on - strand, within Pf1N1B4_1885 at 2157.333 kb on - strand, within Pf1N1B4_1885 at 2157.333 kb on - strand, within Pf1N1B4_1885 at 2157.435 kb on - strand, within Pf1N1B4_1885 at 2157.549 kb on - strand, within Pf1N1B4_1885 at 2157.719 kb on - strand, within Pf1N1B4_1885 at 2157.719 kb on - strand, within Pf1N1B4_1885 at 2157.852 kb on + strand, within Pf1N1B4_1885
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 2,154,244 - Pf1N1B4_1883 0.41 -2.3 2,154,271 - Pf1N1B4_1883 0.43 -0.0 2,154,271 - Pf1N1B4_1883 0.43 -0.0 2,154,271 - Pf1N1B4_1883 0.43 -2.9 2,154,310 + Pf1N1B4_1883 0.46 -0.0 2,154,310 + Pf1N1B4_1883 0.46 -0.0 2,154,311 - Pf1N1B4_1883 0.46 -0.0 2,154,311 - Pf1N1B4_1883 0.46 -0.0 2,154,311 - Pf1N1B4_1883 0.46 -1.6 2,154,319 + Pf1N1B4_1883 0.46 -2.5 2,154,320 - Pf1N1B4_1883 0.46 -0.0 2,154,364 + Pf1N1B4_1883 0.49 -4.1 2,154,365 - Pf1N1B4_1883 0.49 -0.0 2,154,388 + Pf1N1B4_1883 0.50 -1.6 2,154,388 + Pf1N1B4_1883 0.50 -2.4 2,154,445 + Pf1N1B4_1883 0.54 -0.0 2,154,445 + Pf1N1B4_1883 0.54 -0.0 2,154,445 + Pf1N1B4_1883 0.54 -1.9 2,154,445 + Pf1N1B4_1883 0.54 -2.1 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,446 - Pf1N1B4_1883 0.54 -2.4 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,446 - Pf1N1B4_1883 0.54 -0.0 2,154,456 + Pf1N1B4_1883 0.55 -0.0 2,154,456 + Pf1N1B4_1883 0.55 -0.0 2,154,457 - Pf1N1B4_1883 0.55 -0.0 2,154,457 - Pf1N1B4_1883 0.55 -3.2 2,154,457 - Pf1N1B4_1883 0.55 -2.5 2,154,457 - Pf1N1B4_1883 0.55 -0.0 2,154,457 - Pf1N1B4_1883 0.55 -0.0 2,154,457 - Pf1N1B4_1883 0.55 -1.0 2,154,457 - Pf1N1B4_1883 0.55 -0.0 2,154,457 - Pf1N1B4_1883 0.55 -0.0 2,154,557 + Pf1N1B4_1883 0.61 -0.0 2,154,575 + -3.2 2,154,610 + Pf1N1B4_1883 0.64 -2.9 2,154,611 - Pf1N1B4_1883 0.64 -2.7 2,154,611 - Pf1N1B4_1883 0.64 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -2.2 2,154,663 + Pf1N1B4_1883 0.67 -4.5 2,154,663 + Pf1N1B4_1883 0.67 -5.1 2,154,663 + Pf1N1B4_1883 0.67 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -3.0 2,154,663 + Pf1N1B4_1883 0.67 -0.0 2,154,663 + Pf1N1B4_1883 0.67 -3.2 2,154,663 + Pf1N1B4_1883 0.67 -3.1 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -2.8 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -0.9 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,664 - Pf1N1B4_1883 0.67 -2.3 2,154,664 - Pf1N1B4_1883 0.67 -0.0 2,154,876 + Pf1N1B4_1883 0.81 -0.0 2,154,877 - Pf1N1B4_1883 0.81 -2.7 2,154,891 + Pf1N1B4_1883 0.81 -0.0 2,154,939 - Pf1N1B4_1883 0.84 -1.8 2,155,164 - -0.0 2,155,204 + -0.0 2,155,204 + -0.0 2,155,204 + -0.0 2,155,204 + -0.0 2,155,204 + -0.0 2,155,205 - -0.0 2,155,205 - -0.0 2,155,205 - -0.0 2,155,205 - -0.0 2,155,205 - -0.0 2,155,205 - -0.0 2,155,206 + -0.0 2,155,222 + -0.0 2,155,222 + -0.0 2,155,222 + -0.0 2,155,223 - -0.0 2,155,223 - -0.0 2,155,223 - -0.0 2,155,223 - -0.0 2,155,223 - -0.0 2,155,223 - -0.0 2,155,223 - -2.4 2,155,223 - -0.0 2,155,223 - -0.0 2,155,223 - -0.0 2,155,252 + -0.0 2,155,253 - -1.2 2,155,253 - -0.0 2,155,375 + -1.6 2,155,409 + Pf1N1B4_1884 0.12 -0.0 2,155,410 - -1.1 2,155,410 - Pf1N1B4_1884 0.12 -0.0 2,155,446 + Pf1N1B4_1884 0.14 +0.7 2,155,447 - Pf1N1B4_1884 0.14 -0.0 2,155,447 - Pf1N1B4_1884 0.14 -0.0 2,155,926 - Pf1N1B4_1884 0.40 -0.0 2,155,959 - Pf1N1B4_1884 0.42 -0.0 2,155,960 + Pf1N1B4_1884 0.42 -0.0 2,155,960 + Pf1N1B4_1884 0.42 -0.9 2,155,960 + Pf1N1B4_1884 0.42 -0.5 2,155,960 + Pf1N1B4_1884 0.42 -0.0 2,155,960 + Pf1N1B4_1884 0.42 -0.0 2,155,960 + Pf1N1B4_1884 0.42 -1.6 2,155,960 + Pf1N1B4_1884 0.42 -1.3 2,155,960 + Pf1N1B4_1884 0.42 -0.0 2,155,961 - Pf1N1B4_1884 0.42 -2.8 2,155,961 - Pf1N1B4_1884 0.42 -0.4 2,155,961 - Pf1N1B4_1884 0.42 -0.0 2,155,961 - Pf1N1B4_1884 0.42 -1.0 2,155,961 - Pf1N1B4_1884 0.42 -0.0 2,155,961 - Pf1N1B4_1884 0.42 +0.5 2,155,961 - Pf1N1B4_1884 0.42 -0.0 2,155,961 - Pf1N1B4_1884 0.42 -0.0 2,155,961 - Pf1N1B4_1884 0.42 -0.7 2,155,961 - Pf1N1B4_1884 0.42 -0.8 2,155,961 - Pf1N1B4_1884 0.42 -3.3 2,156,035 - Pf1N1B4_1884 0.46 -0.0 2,156,037 + Pf1N1B4_1884 0.47 -0.0 2,156,037 + Pf1N1B4_1884 0.47 -3.1 2,156,038 - Pf1N1B4_1884 0.47 -0.0 2,156,250 - Pf1N1B4_1884 0.58 -0.0 2,156,348 + -0.6 2,156,607 + Pf1N1B4_1884 0.78 -0.0 2,156,608 - Pf1N1B4_1884 0.78 -0.9 2,156,646 + Pf1N1B4_1884 0.81 -1.2 2,156,646 + Pf1N1B4_1884 0.81 -0.0 2,156,647 - Pf1N1B4_1884 0.81 -0.0 2,156,647 - Pf1N1B4_1884 0.81 -1.2 2,156,684 - Pf1N1B4_1884 0.83 -0.0 2,156,936 + -1.9 2,156,937 - -0.0 2,156,937 - -0.0 2,156,937 - -0.0 2,156,937 - -0.0 2,156,937 - -0.0 2,156,937 - -2.0 2,156,952 - -0.0 2,157,014 + -0.0 2,157,014 + -0.0 2,157,014 + -0.0 2,157,015 - -0.0 2,157,015 - -3.8 2,157,015 - -0.0 2,157,222 + Pf1N1B4_1885 0.16 -0.0 2,157,222 + Pf1N1B4_1885 0.16 -0.0 2,157,222 + Pf1N1B4_1885 0.16 -0.0 2,157,222 + Pf1N1B4_1885 0.16 -0.0 2,157,222 + Pf1N1B4_1885 0.16 -0.0 2,157,222 + Pf1N1B4_1885 0.16 -0.4 2,157,222 + Pf1N1B4_1885 0.16 -3.3 2,157,222 + Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 +1.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -2.6 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,223 - Pf1N1B4_1885 0.16 -0.0 2,157,279 - Pf1N1B4_1885 0.20 -0.0 2,157,333 - Pf1N1B4_1885 0.24 -1.0 2,157,333 - Pf1N1B4_1885 0.24 -2.3 2,157,435 - Pf1N1B4_1885 0.32 -0.0 2,157,549 - Pf1N1B4_1885 0.40 -1.8 2,157,719 - Pf1N1B4_1885 0.53 -1.4 2,157,719 - Pf1N1B4_1885 0.53 -0.0 2,157,852 + Pf1N1B4_1885 0.63 -0.0
Or see this region's nucleotide sequence