Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1746 and Pf1N1B4_1747 are separated by 181 nucleotides Pf1N1B4_1747 and Pf1N1B4_1748 are separated by 5 nucleotides Pf1N1B4_1748 and Pf1N1B4_1749 are separated by 232 nucleotides Pf1N1B4_1749 and Pf1N1B4_1750 overlap by 4 nucleotides Pf1N1B4_1750 and Pf1N1B4_1751 are separated by 3 nucleotides
Pf1N1B4_1746 - GTP-binding protein, at 1,998,328 to 1,999,803
_1746
Pf1N1B4_1747 - hypothetical protein, at 1,999,985 to 2,000,116
_1747
Pf1N1B4_1748 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 2,000,122 to 2,000,757
_1748
Pf1N1B4_1749 - Prevent host death protein, Phd antitoxin, at 2,000,990 to 2,001,268
_1749
Pf1N1B4_1750 - Death on curing protein, Doc toxin, at 2,001,265 to 2,001,546
_1750
Pf1N1B4_1751 - FIG00960943: hypothetical protein, at 2,001,550 to 2,001,795
_1751
Position (kb)
2000
2001 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1999.145 kb on + strand, within Pf1N1B4_1746 at 1999.154 kb on + strand, within Pf1N1B4_1746 at 1999.154 kb on + strand, within Pf1N1B4_1746 at 1999.155 kb on - strand, within Pf1N1B4_1746 at 1999.254 kb on + strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.256 kb on + strand, within Pf1N1B4_1746 at 1999.360 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.482 kb on - strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.767 kb on - strand at 1999.813 kb on + strand at 1999.814 kb on - strand at 1999.816 kb on + strand at 1999.816 kb on + strand at 1999.817 kb on - strand at 1999.833 kb on + strand at 1999.833 kb on + strand at 1999.834 kb on - strand at 1999.834 kb on - strand at 1999.834 kb on - strand at 1999.844 kb on + strand at 1999.986 kb on + strand at 2000.129 kb on + strand at 2000.129 kb on + strand at 2000.130 kb on - strand at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.190 kb on - strand, within Pf1N1B4_1748 at 2000.457 kb on - strand, within Pf1N1B4_1748 at 2000.472 kb on - strand, within Pf1N1B4_1748 at 2000.578 kb on + strand, within Pf1N1B4_1748 at 2000.579 kb on - strand, within Pf1N1B4_1748 at 2000.645 kb on - strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.758 kb on + strand at 2000.758 kb on + strand at 2000.759 kb on - strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.829 kb on - strand at 2000.829 kb on - strand at 2000.920 kb on + strand at 2000.920 kb on + strand at 2000.920 kb on + strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.942 kb on + strand at 2000.942 kb on + strand at 2000.942 kb on + strand at 2000.943 kb on - strand at 2000.943 kb on - strand at 2000.943 kb on - strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.955 kb on + strand at 2000.955 kb on + strand at 2000.955 kb on + strand at 2000.956 kb on - strand at 2000.956 kb on - strand at 2000.956 kb on - strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.968 kb on + strand at 2000.969 kb on - strand at 2000.969 kb on - strand at 2000.972 kb on + strand at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.259 kb on - strand at 2001.259 kb on - strand at 2001.259 kb on - strand at 2001.272 kb on + strand at 2001.272 kb on + strand at 2001.273 kb on - strand at 2001.273 kb on - strand at 2001.273 kb on - strand at 2001.444 kb on - strand, within Pf1N1B4_1750 at 2001.516 kb on + strand, within Pf1N1B4_1750 at 2001.517 kb on - strand at 2001.517 kb on - strand, within Pf1N1B4_1750 at 2001.552 kb on - strand at 2001.720 kb on + strand, within Pf1N1B4_1751 at 2001.721 kb on - strand, within Pf1N1B4_1751
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 1,999,145 + Pf1N1B4_1746 0.55 -1.8 1,999,154 + Pf1N1B4_1746 0.56 -0.0 1,999,154 + Pf1N1B4_1746 0.56 -0.0 1,999,155 - Pf1N1B4_1746 0.56 -0.0 1,999,254 + Pf1N1B4_1746 0.63 -0.0 1,999,255 - Pf1N1B4_1746 0.63 -0.0 1,999,255 - Pf1N1B4_1746 0.63 -3.6 1,999,255 - Pf1N1B4_1746 0.63 -3.7 1,999,255 - Pf1N1B4_1746 0.63 -0.0 1,999,256 + Pf1N1B4_1746 0.63 -0.0 1,999,360 + Pf1N1B4_1746 0.70 -2.4 1,999,481 + Pf1N1B4_1746 0.78 -0.0 1,999,481 + Pf1N1B4_1746 0.78 -0.0 1,999,481 + Pf1N1B4_1746 0.78 -0.0 1,999,481 + Pf1N1B4_1746 0.78 -0.0 1,999,482 - Pf1N1B4_1746 0.78 -2.2 1,999,639 + Pf1N1B4_1746 0.89 -2.5 1,999,639 + Pf1N1B4_1746 0.89 -0.0 1,999,639 + Pf1N1B4_1746 0.89 -0.0 1,999,640 - Pf1N1B4_1746 0.89 -0.0 1,999,640 - Pf1N1B4_1746 0.89 -0.0 1,999,640 - Pf1N1B4_1746 0.89 -0.0 1,999,640 - Pf1N1B4_1746 0.89 -1.8 1,999,767 - -0.0 1,999,813 + -0.0 1,999,814 - -0.0 1,999,816 + -0.0 1,999,816 + -2.2 1,999,817 - -0.0 1,999,833 + -0.0 1,999,833 + -2.1 1,999,834 - -0.0 1,999,834 - -3.1 1,999,834 - -1.0 1,999,844 + -5.0 1,999,986 + -0.0 2,000,129 + -0.0 2,000,129 + -0.0 2,000,130 - -0.0 2,000,187 + Pf1N1B4_1748 0.10 -4.2 2,000,187 + Pf1N1B4_1748 0.10 -0.0 2,000,187 + Pf1N1B4_1748 0.10 -0.0 2,000,187 + Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -3.6 2,000,189 + Pf1N1B4_1748 0.11 -0.0 2,000,189 + Pf1N1B4_1748 0.11 -2.7 2,000,189 + Pf1N1B4_1748 0.11 -0.0 2,000,189 + Pf1N1B4_1748 0.11 -0.0 2,000,190 - Pf1N1B4_1748 0.11 -0.0 2,000,457 - Pf1N1B4_1748 0.53 -0.0 2,000,472 - Pf1N1B4_1748 0.55 -0.0 2,000,578 + Pf1N1B4_1748 0.72 -0.0 2,000,579 - Pf1N1B4_1748 0.72 -0.0 2,000,645 - Pf1N1B4_1748 0.82 -0.0 2,000,690 + Pf1N1B4_1748 0.89 -0.0 2,000,690 + Pf1N1B4_1748 0.89 -3.7 2,000,690 + Pf1N1B4_1748 0.89 -1.0 2,000,691 - Pf1N1B4_1748 0.89 -0.0 2,000,691 - Pf1N1B4_1748 0.89 -0.0 2,000,691 - Pf1N1B4_1748 0.89 -0.0 2,000,691 - Pf1N1B4_1748 0.89 -0.0 2,000,691 - Pf1N1B4_1748 0.89 -0.0 2,000,691 - Pf1N1B4_1748 0.89 -2.0 2,000,758 + -0.0 2,000,758 + -0.0 2,000,759 - -0.0 2,000,761 + -2.3 2,000,761 + -0.0 2,000,761 + -0.0 2,000,761 + -0.0 2,000,762 - -0.0 2,000,762 - -2.9 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -1.3 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,762 - -0.0 2,000,828 + -3.8 2,000,828 + -0.0 2,000,828 + -0.0 2,000,828 + -3.2 2,000,829 - -0.0 2,000,829 - -0.0 2,000,920 + -0.0 2,000,920 + +0.3 2,000,920 + -0.0 2,000,921 - -0.0 2,000,921 - -3.3 2,000,921 - -0.0 2,000,921 - -1.2 2,000,942 + -1.4 2,000,942 + -0.0 2,000,942 + -0.0 2,000,943 - -0.0 2,000,943 - -3.3 2,000,943 - -0.0 2,000,953 + -2.2 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -0.8 2,000,953 + -3.0 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -1.2 2,000,953 + -2.6 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -0.0 2,000,953 + -2.5 2,000,953 + -0.0 2,000,953 + -1.2 2,000,954 - -1.7 2,000,954 - -0.0 2,000,954 - -3.0 2,000,954 - -0.0 2,000,954 - -0.0 2,000,954 - -0.0 2,000,954 - -2.2 2,000,954 - -0.0 2,000,954 - -0.0 2,000,954 - -2.8 2,000,954 - -0.0 2,000,954 - -2.4 2,000,954 - -2.7 2,000,954 - -0.0 2,000,954 - -4.9 2,000,954 - -0.0 2,000,954 - -0.0 2,000,954 - -2.5 2,000,954 - -0.0 2,000,955 + -2.5 2,000,955 + -0.0 2,000,955 + -0.0 2,000,956 - -0.0 2,000,956 - -0.0 2,000,956 - -2.7 2,000,957 + -1.6 2,000,957 + -1.0 2,000,957 + -1.4 2,000,957 + -0.0 2,000,957 + -0.0 2,000,957 + -0.0 2,000,957 + -2.8 2,000,958 - -0.0 2,000,958 - -1.6 2,000,958 - -0.0 2,000,958 - -4.8 2,000,958 - -0.0 2,000,958 - -0.0 2,000,958 - -0.0 2,000,958 - -2.8 2,000,958 - -0.0 2,000,958 - -0.0 2,000,958 - -0.0 2,000,958 - -0.0 2,000,958 - -0.0 2,000,968 + -0.0 2,000,969 - -0.0 2,000,969 - -0.0 2,000,972 + -4.9 2,001,161 - Pf1N1B4_1749 0.61 -3.1 2,001,161 - Pf1N1B4_1749 0.61 -3.4 2,001,161 - Pf1N1B4_1749 0.61 -0.0 2,001,161 - -3.0 2,001,257 - -0.0 2,001,257 - -2.6 2,001,257 - -0.0 2,001,257 - -0.0 2,001,257 - -0.0 2,001,257 - -0.0 2,001,258 + -0.0 2,001,258 + -0.0 2,001,258 + -2.5 2,001,258 + -0.0 2,001,258 + -0.6 2,001,259 - -0.0 2,001,259 - -2.9 2,001,259 - -1.8 2,001,272 + -3.0 2,001,272 + -0.0 2,001,273 - -0.0 2,001,273 - -0.0 2,001,273 - -0.0 2,001,444 - Pf1N1B4_1750 0.63 -0.0 2,001,516 + Pf1N1B4_1750 0.89 -0.5 2,001,517 - -2.3 2,001,517 - Pf1N1B4_1750 0.89 -0.0 2,001,552 - -2.4 2,001,720 + Pf1N1B4_1751 0.69 -0.0 2,001,721 - Pf1N1B4_1751 0.70 -1.9
Or see this region's nucleotide sequence