Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1672 and Pf1N1B4_1673 are separated by 91 nucleotides Pf1N1B4_1673 and Pf1N1B4_1674 overlap by 4 nucleotides
Pf1N1B4_1672 - Hydrogen peroxide-inducible genes activator, at 1,915,286 to 1,916,173
_1672
Pf1N1B4_1673 - Formate dehydrogenase chain D (EC 1.2.1.2), at 1,916,265 to 1,917,104
_1673
Pf1N1B4_1674 - Putative formate dehydrogenase oxidoreductase protein, at 1,917,101 to 1,919,449
_1674
Position (kb)
1916
1917
1918 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1915.509 kb on - strand, within Pf1N1B4_1672 at 1915.558 kb on + strand, within Pf1N1B4_1672 at 1915.721 kb on + strand, within Pf1N1B4_1672 at 1915.722 kb on - strand, within Pf1N1B4_1672 at 1915.790 kb on + strand, within Pf1N1B4_1672 at 1915.791 kb on - strand, within Pf1N1B4_1672 at 1915.791 kb on - strand, within Pf1N1B4_1672 at 1915.827 kb on + strand, within Pf1N1B4_1672 at 1915.827 kb on + strand, within Pf1N1B4_1672 at 1915.827 kb on + strand, within Pf1N1B4_1672 at 1915.903 kb on + strand, within Pf1N1B4_1672 at 1915.903 kb on + strand, within Pf1N1B4_1672 at 1915.903 kb on + strand, within Pf1N1B4_1672 at 1915.968 kb on + strand, within Pf1N1B4_1672 at 1915.968 kb on + strand, within Pf1N1B4_1672 at 1915.968 kb on + strand, within Pf1N1B4_1672 at 1915.978 kb on - strand, within Pf1N1B4_1672 at 1916.022 kb on + strand, within Pf1N1B4_1672 at 1916.022 kb on + strand, within Pf1N1B4_1672 at 1916.023 kb on - strand, within Pf1N1B4_1672 at 1916.023 kb on - strand, within Pf1N1B4_1672 at 1916.043 kb on + strand, within Pf1N1B4_1672 at 1916.043 kb on + strand, within Pf1N1B4_1672 at 1916.043 kb on + strand, within Pf1N1B4_1672 at 1916.044 kb on - strand, within Pf1N1B4_1672 at 1916.044 kb on - strand, within Pf1N1B4_1672 at 1916.044 kb on - strand, within Pf1N1B4_1672 at 1916.104 kb on + strand at 1916.147 kb on + strand at 1916.147 kb on + strand at 1916.147 kb on + strand at 1916.147 kb on + strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.186 kb on - strand at 1916.200 kb on - strand at 1916.202 kb on + strand at 1916.202 kb on + strand at 1916.202 kb on + strand at 1916.202 kb on + strand at 1916.233 kb on - strand at 1916.233 kb on - strand at 1916.233 kb on - strand at 1916.239 kb on - strand at 1916.239 kb on - strand at 1916.239 kb on - strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.335 kb on - strand at 1916.335 kb on - strand at 1916.340 kb on + strand at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.513 kb on + strand, within Pf1N1B4_1673 at 1916.517 kb on + strand, within Pf1N1B4_1673 at 1916.517 kb on + strand, within Pf1N1B4_1673 at 1916.517 kb on + strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.780 kb on - strand, within Pf1N1B4_1673 at 1916.986 kb on + strand, within Pf1N1B4_1673 at 1917.075 kb on + strand at 1917.076 kb on - strand at 1917.076 kb on - strand at 1917.152 kb on + strand at 1917.152 kb on + strand at 1917.152 kb on + strand at 1917.153 kb on - strand at 1917.153 kb on - strand at 1917.240 kb on + strand at 1917.241 kb on - strand at 1917.340 kb on + strand, within Pf1N1B4_1674 at 1917.400 kb on + strand, within Pf1N1B4_1674 at 1917.400 kb on + strand, within Pf1N1B4_1674 at 1917.400 kb on + strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.443 kb on + strand, within Pf1N1B4_1674 at 1917.443 kb on + strand, within Pf1N1B4_1674 at 1917.444 kb on - strand, within Pf1N1B4_1674 at 1917.444 kb on - strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.578 kb on + strand, within Pf1N1B4_1674 at 1917.578 kb on + strand, within Pf1N1B4_1674 at 1917.578 kb on + strand, within Pf1N1B4_1674 at 1917.579 kb on - strand, within Pf1N1B4_1674 at 1917.579 kb on - strand, within Pf1N1B4_1674 at 1917.618 kb on - strand, within Pf1N1B4_1674 at 1917.954 kb on - strand, within Pf1N1B4_1674 at 1917.954 kb on - strand, within Pf1N1B4_1674 at 1918.103 kb on + strand, within Pf1N1B4_1674 at 1918.103 kb on + strand, within Pf1N1B4_1674 at 1918.103 kb on + strand, within Pf1N1B4_1674 at 1918.104 kb on - strand, within Pf1N1B4_1674
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 1,915,509 - Pf1N1B4_1672 0.25 -2.3 1,915,558 + Pf1N1B4_1672 0.31 +0.0 1,915,721 + Pf1N1B4_1672 0.49 +0.0 1,915,722 - Pf1N1B4_1672 0.49 +0.0 1,915,790 + Pf1N1B4_1672 0.57 -4.5 1,915,791 - Pf1N1B4_1672 0.57 -1.9 1,915,791 - Pf1N1B4_1672 0.57 -1.6 1,915,827 + Pf1N1B4_1672 0.61 +0.0 1,915,827 + Pf1N1B4_1672 0.61 +0.0 1,915,827 + Pf1N1B4_1672 0.61 +0.0 1,915,903 + Pf1N1B4_1672 0.69 -1.8 1,915,903 + Pf1N1B4_1672 0.69 +0.0 1,915,903 + Pf1N1B4_1672 0.69 -2.2 1,915,968 + Pf1N1B4_1672 0.77 +0.0 1,915,968 + Pf1N1B4_1672 0.77 -2.4 1,915,968 + Pf1N1B4_1672 0.77 +0.0 1,915,978 - Pf1N1B4_1672 0.78 +0.0 1,916,022 + Pf1N1B4_1672 0.83 +0.0 1,916,022 + Pf1N1B4_1672 0.83 +0.0 1,916,023 - Pf1N1B4_1672 0.83 +0.0 1,916,023 - Pf1N1B4_1672 0.83 +0.0 1,916,043 + Pf1N1B4_1672 0.85 +0.0 1,916,043 + Pf1N1B4_1672 0.85 +0.0 1,916,043 + Pf1N1B4_1672 0.85 -2.2 1,916,044 - Pf1N1B4_1672 0.85 -1.0 1,916,044 - Pf1N1B4_1672 0.85 +0.0 1,916,044 - Pf1N1B4_1672 0.85 -2.6 1,916,104 + -3.0 1,916,147 + +0.0 1,916,147 + +0.0 1,916,147 + +0.0 1,916,147 + +0.0 1,916,148 - -1.4 1,916,148 - +0.0 1,916,148 - +0.0 1,916,148 - -3.5 1,916,148 - +0.0 1,916,148 - +0.0 1,916,148 - +0.0 1,916,186 - -3.8 1,916,200 - -2.0 1,916,202 + +0.0 1,916,202 + -1.5 1,916,202 + +0.0 1,916,202 + -2.9 1,916,233 - +0.0 1,916,233 - +0.0 1,916,233 - +0.0 1,916,239 - +0.0 1,916,239 - +0.0 1,916,239 - +0.0 1,916,334 + -0.7 1,916,334 + -1.2 1,916,334 + -0.6 1,916,334 + +0.0 1,916,334 + +0.0 1,916,334 + -1.4 1,916,334 + +0.0 1,916,335 - +0.0 1,916,335 - -2.2 1,916,340 + +0.0 1,916,355 + Pf1N1B4_1673 0.11 -2.2 1,916,355 + Pf1N1B4_1673 0.11 -4.0 1,916,355 + Pf1N1B4_1673 0.11 +0.0 1,916,355 + Pf1N1B4_1673 0.11 +0.0 1,916,355 + Pf1N1B4_1673 0.11 +0.0 1,916,355 + Pf1N1B4_1673 0.11 +0.0 1,916,356 - Pf1N1B4_1673 0.11 +0.0 1,916,356 - Pf1N1B4_1673 0.11 +0.0 1,916,356 - Pf1N1B4_1673 0.11 +0.0 1,916,356 - Pf1N1B4_1673 0.11 +0.0 1,916,356 - Pf1N1B4_1673 0.11 +0.0 1,916,356 - Pf1N1B4_1673 0.11 +0.0 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,410 - Pf1N1B4_1673 0.17 -2.0 1,916,410 - Pf1N1B4_1673 0.17 +0.0 1,916,410 - Pf1N1B4_1673 0.17 -1.7 1,916,410 - Pf1N1B4_1673 0.17 +0.0 1,916,410 - Pf1N1B4_1673 0.17 +0.0 1,916,513 + Pf1N1B4_1673 0.30 +0.0 1,916,517 + Pf1N1B4_1673 0.30 +0.0 1,916,517 + Pf1N1B4_1673 0.30 -1.2 1,916,517 + Pf1N1B4_1673 0.30 +0.0 1,916,518 - Pf1N1B4_1673 0.30 +0.0 1,916,518 - Pf1N1B4_1673 0.30 -0.2 1,916,518 - Pf1N1B4_1673 0.30 +0.0 1,916,518 - Pf1N1B4_1673 0.30 +0.0 1,916,518 - Pf1N1B4_1673 0.30 -1.1 1,916,554 - Pf1N1B4_1673 0.34 -2.0 1,916,554 - Pf1N1B4_1673 0.34 +0.0 1,916,554 - Pf1N1B4_1673 0.34 -0.9 1,916,554 - Pf1N1B4_1673 0.34 +0.0 1,916,780 - Pf1N1B4_1673 0.61 -0.6 1,916,986 + Pf1N1B4_1673 0.86 -0.9 1,917,075 + +0.0 1,917,076 - +0.0 1,917,076 - +0.0 1,917,152 + +0.0 1,917,152 + -2.7 1,917,152 + -2.0 1,917,153 - +0.0 1,917,153 - +0.0 1,917,240 + +0.0 1,917,241 - +0.0 1,917,340 + Pf1N1B4_1674 0.10 +0.0 1,917,400 + Pf1N1B4_1674 0.13 +0.0 1,917,400 + Pf1N1B4_1674 0.13 +0.0 1,917,400 + Pf1N1B4_1674 0.13 +0.0 1,917,401 - Pf1N1B4_1674 0.13 -3.1 1,917,401 - Pf1N1B4_1674 0.13 +0.0 1,917,401 - Pf1N1B4_1674 0.13 +0.0 1,917,401 - Pf1N1B4_1674 0.13 +0.0 1,917,443 + Pf1N1B4_1674 0.15 +0.0 1,917,443 + Pf1N1B4_1674 0.15 -1.3 1,917,444 - Pf1N1B4_1674 0.15 +0.0 1,917,444 - Pf1N1B4_1674 0.15 -3.2 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 -2.8 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 -2.3 1,917,474 - Pf1N1B4_1674 0.16 -2.2 1,917,474 - Pf1N1B4_1674 0.16 -2.6 1,917,474 - Pf1N1B4_1674 0.16 +0.0 1,917,474 - Pf1N1B4_1674 0.16 +0.0 1,917,474 - Pf1N1B4_1674 0.16 +0.0 1,917,474 - Pf1N1B4_1674 0.16 -2.9 1,917,578 + Pf1N1B4_1674 0.20 -2.8 1,917,578 + Pf1N1B4_1674 0.20 +0.0 1,917,578 + Pf1N1B4_1674 0.20 +0.0 1,917,579 - Pf1N1B4_1674 0.20 +0.0 1,917,579 - Pf1N1B4_1674 0.20 +0.0 1,917,618 - Pf1N1B4_1674 0.22 +0.0 1,917,954 - Pf1N1B4_1674 0.36 -3.0 1,917,954 - Pf1N1B4_1674 0.36 +0.0 1,918,103 + Pf1N1B4_1674 0.43 +0.0 1,918,103 + Pf1N1B4_1674 0.43 -3.3 1,918,103 + Pf1N1B4_1674 0.43 -1.8 1,918,104 - Pf1N1B4_1674 0.43 +0.0
Or see this region's nucleotide sequence