Experiment: Nitrate reduction, replicate C t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1557 and Pf1N1B4_1558 are separated by 59 nucleotides Pf1N1B4_1558 and Pf1N1B4_1559 are separated by 270 nucleotides Pf1N1B4_1559 and Pf1N1B4_1560 are separated by 10 nucleotides Pf1N1B4_1560 and Pf1N1B4_1561 are separated by 248 nucleotides
Pf1N1B4_1557 - Polyhydroxyalkanoic acid synthase, at 1,796,052 to 1,797,734
_1557
Pf1N1B4_1558 - Transcriptional regulator PhaD, at 1,797,794 to 1,798,414
_1558
Pf1N1B4_1559 - Polyhydroxyalkanoate granule-associated protein PhaF, at 1,798,685 to 1,799,473
_1559
Pf1N1B4_1560 - polyhydroxyalkanoate granule-associated protein PhaI, at 1,799,484 to 1,799,906
_1560
Pf1N1B4_1561 - FIG00954300: hypothetical protein, at 1,800,155 to 1,800,430
_1561
Position (kb)
1798
1799
1800 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1797.687 kb on + strand at 1797.688 kb on - strand at 1797.714 kb on - strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.717 kb on + strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.718 kb on - strand at 1797.740 kb on - strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.782 kb on + strand at 1797.911 kb on + strand, within Pf1N1B4_1558 at 1797.912 kb on - strand, within Pf1N1B4_1558 at 1797.912 kb on - strand at 1797.915 kb on - strand, within Pf1N1B4_1558 at 1798.026 kb on - strand, within Pf1N1B4_1558 at 1798.275 kb on - strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.319 kb on + strand, within Pf1N1B4_1558 at 1798.445 kb on + strand at 1798.445 kb on + strand at 1798.446 kb on - strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.526 kb on + strand at 1798.782 kb on - strand, within Pf1N1B4_1559 at 1798.904 kb on - strand, within Pf1N1B4_1559 at 1798.939 kb on + strand, within Pf1N1B4_1559 at 1799.339 kb on - strand, within Pf1N1B4_1559 at 1799.385 kb on - strand, within Pf1N1B4_1559 at 1799.413 kb on - strand at 1799.413 kb on - strand at 1799.493 kb on - strand at 1799.535 kb on - strand, within Pf1N1B4_1560 at 1799.804 kb on - strand, within Pf1N1B4_1560 at 1799.804 kb on - strand, within Pf1N1B4_1560 at 1799.804 kb on - strand, within Pf1N1B4_1560 at 1799.894 kb on + strand at 1799.970 kb on + strand at 1799.979 kb on + strand at 1799.979 kb on + strand at 1799.979 kb on + strand at 1799.980 kb on - strand at 1799.984 kb on - strand at 1799.997 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.009 kb on + strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.010 kb on - strand at 1800.017 kb on + strand at 1800.017 kb on + strand at 1800.017 kb on + strand at 1800.017 kb on + strand at 1800.017 kb on + strand at 1800.017 kb on + strand at 1800.017 kb on + strand at 1800.018 kb on - strand at 1800.018 kb on - strand at 1800.018 kb on - strand at 1800.018 kb on - strand at 1800.021 kb on + strand at 1800.022 kb on - strand at 1800.022 kb on - strand at 1800.022 kb on - strand at 1800.067 kb on + strand at 1800.067 kb on + strand at 1800.067 kb on + strand at 1800.068 kb on - strand at 1800.078 kb on + strand at 1800.078 kb on + strand at 1800.079 kb on - strand at 1800.079 kb on - strand at 1800.097 kb on - strand at 1800.097 kb on - strand at 1800.268 kb on - strand at 1800.268 kb on - strand, within Pf1N1B4_1561 at 1800.268 kb on - strand, within Pf1N1B4_1561 at 1800.268 kb on - strand, within Pf1N1B4_1561 at 1800.268 kb on - strand, within Pf1N1B4_1561 at 1800.268 kb on - strand, within Pf1N1B4_1561 at 1800.268 kb on - strand, within Pf1N1B4_1561 at 1800.281 kb on + strand, within Pf1N1B4_1561 at 1800.281 kb on + strand at 1800.282 kb on - strand, within Pf1N1B4_1561 at 1800.282 kb on - strand, within Pf1N1B4_1561 at 1800.418 kb on + strand at 1800.418 kb on + strand at 1800.418 kb on + strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.419 kb on - strand at 1800.421 kb on + strand at 1800.421 kb on + strand at 1800.422 kb on - strand at 1800.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t2 remove 1,797,687 + +0.0 1,797,688 - +0.0 1,797,714 - -1.3 1,797,717 + +0.0 1,797,717 + -2.1 1,797,717 + +0.0 1,797,717 + +0.0 1,797,718 - -0.8 1,797,718 - +0.0 1,797,718 - +0.0 1,797,718 - +0.0 1,797,718 - -1.1 1,797,718 - +0.0 1,797,718 - -1.6 1,797,718 - -1.9 1,797,740 - +0.0 1,797,782 + +0.0 1,797,782 + -2.7 1,797,782 + -1.8 1,797,911 + Pf1N1B4_1558 0.19 -2.2 1,797,912 - Pf1N1B4_1558 0.19 +0.0 1,797,912 - +0.0 1,797,915 - Pf1N1B4_1558 0.19 +0.0 1,798,026 - Pf1N1B4_1558 0.37 +0.0 1,798,275 - Pf1N1B4_1558 0.77 +1.1 1,798,319 + Pf1N1B4_1558 0.85 -5.3 1,798,319 + Pf1N1B4_1558 0.85 +0.0 1,798,319 + Pf1N1B4_1558 0.85 +0.0 1,798,319 + Pf1N1B4_1558 0.85 -2.1 1,798,445 + -3.1 1,798,445 + +0.0 1,798,446 - +0.0 1,798,526 + +0.0 1,798,526 + +0.0 1,798,526 + +0.0 1,798,526 + +0.0 1,798,782 - Pf1N1B4_1559 0.12 +0.0 1,798,904 - Pf1N1B4_1559 0.28 +0.0 1,798,939 + Pf1N1B4_1559 0.32 -3.4 1,799,339 - Pf1N1B4_1559 0.83 +0.0 1,799,385 - Pf1N1B4_1559 0.89 +0.0 1,799,413 - +0.0 1,799,413 - +0.0 1,799,493 - +0.0 1,799,535 - Pf1N1B4_1560 0.12 +0.0 1,799,804 - Pf1N1B4_1560 0.76 +0.0 1,799,804 - Pf1N1B4_1560 0.76 +0.0 1,799,804 - Pf1N1B4_1560 0.76 -5.0 1,799,894 + +0.0 1,799,970 + -4.4 1,799,979 + +0.0 1,799,979 + -4.8 1,799,979 + -2.8 1,799,980 - -4.0 1,799,984 - -3.6 1,799,997 + +0.1 1,800,009 + +0.0 1,800,009 + +0.0 1,800,009 + -1.9 1,800,009 + -2.0 1,800,009 + +0.0 1,800,009 + +0.0 1,800,009 + +0.0 1,800,009 + +0.0 1,800,010 - -4.8 1,800,010 - +0.0 1,800,010 - -2.4 1,800,010 - +0.0 1,800,010 - +0.0 1,800,010 - +0.0 1,800,010 - +0.0 1,800,010 - +0.0 1,800,010 - +0.0 1,800,017 + -2.7 1,800,017 + +0.0 1,800,017 + -2.0 1,800,017 + -3.2 1,800,017 + -3.0 1,800,017 + -2.9 1,800,017 + +0.0 1,800,018 - +1.0 1,800,018 - -1.5 1,800,018 - +0.0 1,800,018 - -1.9 1,800,021 + -3.7 1,800,022 - -1.2 1,800,022 - -1.3 1,800,022 - -3.1 1,800,067 + -2.6 1,800,067 + -3.8 1,800,067 + -2.1 1,800,068 - -3.6 1,800,078 + +0.0 1,800,078 + -1.9 1,800,079 - +0.0 1,800,079 - +0.0 1,800,097 - +0.0 1,800,097 - +0.0 1,800,268 - +0.0 1,800,268 - Pf1N1B4_1561 0.41 +0.0 1,800,268 - Pf1N1B4_1561 0.41 +0.0 1,800,268 - Pf1N1B4_1561 0.41 +0.0 1,800,268 - Pf1N1B4_1561 0.41 +0.0 1,800,268 - Pf1N1B4_1561 0.41 +0.0 1,800,268 - Pf1N1B4_1561 0.41 +0.0 1,800,281 + Pf1N1B4_1561 0.46 +0.0 1,800,281 + -2.6 1,800,282 - Pf1N1B4_1561 0.46 +0.0 1,800,282 - Pf1N1B4_1561 0.46 +0.0 1,800,418 + +0.0 1,800,418 + -2.7 1,800,418 + +0.0 1,800,419 - +0.0 1,800,419 - -1.0 1,800,419 - -1.4 1,800,419 - -0.8 1,800,419 - +0.0 1,800,419 - +0.0 1,800,419 - -3.2 1,800,419 - +0.0 1,800,419 - +0.0 1,800,419 - +0.0 1,800,419 - -2.7 1,800,421 + +0.0 1,800,421 + -1.5 1,800,422 - +0.0 1,800,447 + -1.4
Or see this region's nucleotide sequence