Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5961 and Pf1N1B4_5962 overlap by 42 nucleotides Pf1N1B4_5962 and Pf1N1B4_5963 are separated by 233 nucleotides
Pf1N1B4_5961 - Major facilitator superfamily transporter, at 409,546 to 411,222
_5961
Pf1N1B4_5962 - hypothetical protein, at 411,181 to 411,342
_5962
Pf1N1B4_5963 - Predicted regulator PutR for proline utilization, GntR family, at 411,576 to 412,247
_5963
Position (kb) 411
412 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 410.201 kb on + strand, within Pf1N1B4_5961 at 410.232 kb on - strand, within Pf1N1B4_5961 at 410.248 kb on + strand, within Pf1N1B4_5961 at 410.249 kb on - strand, within Pf1N1B4_5961 at 410.405 kb on + strand, within Pf1N1B4_5961 at 410.420 kb on + strand, within Pf1N1B4_5961 at 410.470 kb on + strand, within Pf1N1B4_5961 at 410.582 kb on + strand at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.778 kb on - strand at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 411.205 kb on - strand at 411.248 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.268 kb on - strand, within Pf1N1B4_5962 at 411.394 kb on + strand at 411.435 kb on - strand at 411.490 kb on - strand at 411.490 kb on - strand at 411.614 kb on + strand at 411.614 kb on + strand at 411.877 kb on + strand, within Pf1N1B4_5963 at 411.878 kb on - strand, within Pf1N1B4_5963 at 412.062 kb on - strand, within Pf1N1B4_5963 at 412.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 410,201 + Pf1N1B4_5961 0.39 -2.3 410,232 - Pf1N1B4_5961 0.41 +0.0 410,248 + Pf1N1B4_5961 0.42 -4.1 410,249 - Pf1N1B4_5961 0.42 +0.0 410,405 + Pf1N1B4_5961 0.51 -2.5 410,420 + Pf1N1B4_5961 0.52 +0.0 410,470 + Pf1N1B4_5961 0.55 -3.4 410,582 + +0.0 410,777 + Pf1N1B4_5961 0.73 +0.0 410,777 + Pf1N1B4_5961 0.73 -2.6 410,778 - +0.0 410,778 - Pf1N1B4_5961 0.73 +0.0 410,778 - Pf1N1B4_5961 0.73 -2.5 410,778 - Pf1N1B4_5961 0.73 +0.0 410,778 - Pf1N1B4_5961 0.73 -1.7 410,937 + Pf1N1B4_5961 0.83 -0.8 410,937 + Pf1N1B4_5961 0.83 -2.0 410,937 + Pf1N1B4_5961 0.83 +0.0 410,938 - +0.0 410,938 - Pf1N1B4_5961 0.83 +0.0 410,938 - +0.0 410,938 - Pf1N1B4_5961 0.83 +0.0 410,938 - Pf1N1B4_5961 0.83 -2.0 410,938 - Pf1N1B4_5961 0.83 -2.4 410,996 - Pf1N1B4_5961 0.86 -1.7 410,996 - Pf1N1B4_5961 0.86 -2.5 411,205 - +0.0 411,248 - Pf1N1B4_5962 0.41 -3.0 411,250 - Pf1N1B4_5962 0.43 -3.0 411,250 - Pf1N1B4_5962 0.43 -3.4 411,250 - Pf1N1B4_5962 0.43 -2.4 411,250 - Pf1N1B4_5962 0.43 -2.2 411,268 - Pf1N1B4_5962 0.54 +0.0 411,394 + +0.0 411,435 - -2.1 411,490 - +0.0 411,490 - +0.0 411,614 + -4.3 411,614 + +0.0 411,877 + Pf1N1B4_5963 0.45 -5.5 411,878 - Pf1N1B4_5963 0.45 +0.0 412,062 - Pf1N1B4_5963 0.72 +0.0 412,281 - -1.3
Or see this region's nucleotide sequence