Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5935 and Pf1N1B4_5936 are separated by 210 nucleotides Pf1N1B4_5936 and Pf1N1B4_5937 are separated by 426 nucleotides Pf1N1B4_5937 and Pf1N1B4_5938 overlap by 4 nucleotides Pf1N1B4_5938 and Pf1N1B4_5939 are separated by 45 nucleotides
Pf1N1B4_5935 - Polysaccharide export lipoprotein Wza, at 380,161 to 381,150
_5935
Pf1N1B4_5936 - hypothetical protein, at 381,361 to 381,633
_5936
Pf1N1B4_5937 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 382,060 to 382,599
_5937
Pf1N1B4_5938 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 382,596 to 383,474
_5938
Pf1N1B4_5939 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 383,520 to 384,587
_5939
Position (kb) 382
383 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 381.363 kb on - strand at 381.363 kb on - strand at 381.363 kb on - strand at 381.659 kb on - strand at 381.666 kb on - strand at 381.675 kb on - strand at 381.675 kb on - strand at 381.675 kb on - strand at 381.706 kb on - strand at 381.706 kb on - strand at 381.742 kb on - strand at 381.742 kb on - strand at 381.772 kb on - strand at 381.774 kb on - strand at 381.774 kb on - strand at 381.945 kb on - strand at 381.986 kb on - strand at 381.986 kb on - strand at 381.986 kb on - strand at 382.012 kb on - strand at 382.012 kb on - strand at 382.037 kb on - strand at 382.037 kb on - strand at 382.041 kb on - strand at 382.041 kb on - strand at 382.043 kb on - strand at 382.043 kb on - strand at 382.051 kb on - strand at 382.118 kb on - strand, within Pf1N1B4_5937 at 382.194 kb on - strand, within Pf1N1B4_5937 at 382.194 kb on - strand, within Pf1N1B4_5937 at 382.194 kb on - strand, within Pf1N1B4_5937 at 382.257 kb on - strand, within Pf1N1B4_5937 at 382.355 kb on - strand, within Pf1N1B4_5937 at 382.512 kb on - strand, within Pf1N1B4_5937 at 382.568 kb on - strand at 382.614 kb on - strand at 382.865 kb on - strand, within Pf1N1B4_5938 at 382.865 kb on - strand, within Pf1N1B4_5938 at 382.949 kb on - strand, within Pf1N1B4_5938 at 382.949 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 383.141 kb on - strand, within Pf1N1B4_5938 at 383.174 kb on + strand, within Pf1N1B4_5938 at 383.175 kb on - strand, within Pf1N1B4_5938 at 383.322 kb on - strand, within Pf1N1B4_5938 at 383.322 kb on - strand at 383.329 kb on - strand, within Pf1N1B4_5938 at 383.356 kb on - strand at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.390 kb on - strand at 383.413 kb on - strand at 383.427 kb on - strand at 383.597 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 381,363 - -3.4 381,363 - +0.0 381,363 - +0.0 381,659 - -1.3 381,666 - +0.0 381,675 - +0.0 381,675 - -4.0 381,675 - -3.1 381,706 - -1.6 381,706 - +0.0 381,742 - +0.0 381,742 - -1.4 381,772 - -1.5 381,774 - +0.0 381,774 - +0.0 381,945 - -1.6 381,986 - -1.4 381,986 - -2.1 381,986 - -1.7 382,012 - -2.0 382,012 - -1.7 382,037 - +0.0 382,037 - -1.5 382,041 - +0.0 382,041 - -0.4 382,043 - +0.0 382,043 - -0.5 382,051 - +0.0 382,118 - Pf1N1B4_5937 0.11 -2.1 382,194 - Pf1N1B4_5937 0.25 +0.0 382,194 - Pf1N1B4_5937 0.25 -1.7 382,194 - Pf1N1B4_5937 0.25 +0.0 382,257 - Pf1N1B4_5937 0.36 -2.5 382,355 - Pf1N1B4_5937 0.55 +0.0 382,512 - Pf1N1B4_5937 0.84 -2.7 382,568 - -0.6 382,614 - -2.2 382,865 - Pf1N1B4_5938 0.31 +0.0 382,865 - Pf1N1B4_5938 0.31 +0.0 382,949 - Pf1N1B4_5938 0.40 -1.6 382,949 - Pf1N1B4_5938 0.40 -2.4 382,955 - Pf1N1B4_5938 0.41 -0.4 382,955 - Pf1N1B4_5938 0.41 +0.0 382,955 - Pf1N1B4_5938 0.41 +0.0 383,141 - Pf1N1B4_5938 0.62 +0.0 383,174 + Pf1N1B4_5938 0.66 +0.0 383,175 - Pf1N1B4_5938 0.66 -1.3 383,322 - Pf1N1B4_5938 0.83 -2.3 383,322 - -1.5 383,329 - Pf1N1B4_5938 0.83 +0.0 383,356 - -1.4 383,372 - Pf1N1B4_5938 0.88 +0.0 383,372 - Pf1N1B4_5938 0.88 -2.3 383,372 - Pf1N1B4_5938 0.88 +0.0 383,390 - +0.0 383,413 - +0.0 383,427 - -1.1 383,597 - +0.0
Or see this region's nucleotide sequence