Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5781 and Pf1N1B4_5782 are separated by 55 nucleotides Pf1N1B4_5782 and Pf1N1B4_5783 are separated by 76 nucleotides
Pf1N1B4_5781 - Nitrous-oxide reductase (EC 1.7.99.6), at 217,864 to 219,783
_5781
Pf1N1B4_5782 - Nitrous oxide reductase maturation protein NosR, at 219,839 to 222,052
_5782
Pf1N1B4_5783 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 222,129 to 223,517
_5783
Position (kb) 219
220
221
222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 218.997 kb on - strand, within Pf1N1B4_5781 at 219.212 kb on + strand, within Pf1N1B4_5781 at 219.213 kb on - strand, within Pf1N1B4_5781 at 219.213 kb on - strand, within Pf1N1B4_5781 at 219.213 kb on - strand, within Pf1N1B4_5781 at 219.221 kb on + strand at 219.222 kb on - strand, within Pf1N1B4_5781 at 219.351 kb on - strand at 219.351 kb on - strand at 219.806 kb on - strand at 219.806 kb on - strand at 219.951 kb on + strand at 220.141 kb on - strand, within Pf1N1B4_5782 at 220.149 kb on + strand, within Pf1N1B4_5782 at 220.323 kb on + strand, within Pf1N1B4_5782 at 220.324 kb on - strand, within Pf1N1B4_5782 at 220.599 kb on + strand at 220.632 kb on + strand, within Pf1N1B4_5782 at 220.632 kb on + strand, within Pf1N1B4_5782 at 220.632 kb on + strand, within Pf1N1B4_5782 at 220.633 kb on - strand, within Pf1N1B4_5782 at 220.834 kb on - strand, within Pf1N1B4_5782 at 221.101 kb on - strand, within Pf1N1B4_5782 at 221.113 kb on + strand, within Pf1N1B4_5782 at 221.116 kb on - strand, within Pf1N1B4_5782 at 221.244 kb on + strand, within Pf1N1B4_5782 at 221.245 kb on - strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.684 kb on + strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.689 kb on - strand, within Pf1N1B4_5782 at 222.056 kb on - strand at 222.493 kb on + strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.655 kb on + strand, within Pf1N1B4_5783 at 222.776 kb on - strand, within Pf1N1B4_5783 at 222.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 218,997 - Pf1N1B4_5781 0.59 -3.1 219,212 + Pf1N1B4_5781 0.70 -2.3 219,213 - Pf1N1B4_5781 0.70 +0.1 219,213 - Pf1N1B4_5781 0.70 +0.1 219,213 - Pf1N1B4_5781 0.70 -1.8 219,221 + +0.1 219,222 - Pf1N1B4_5781 0.71 -3.3 219,351 - +0.1 219,351 - -1.9 219,806 - -1.4 219,806 - -0.9 219,951 + -2.8 220,141 - Pf1N1B4_5782 0.14 -2.4 220,149 + Pf1N1B4_5782 0.14 +0.0 220,323 + Pf1N1B4_5782 0.22 -2.4 220,324 - Pf1N1B4_5782 0.22 +0.0 220,599 + -3.0 220,632 + Pf1N1B4_5782 0.36 -2.6 220,632 + Pf1N1B4_5782 0.36 +0.0 220,632 + Pf1N1B4_5782 0.36 +0.0 220,633 - Pf1N1B4_5782 0.36 +0.0 220,834 - Pf1N1B4_5782 0.45 +0.0 221,101 - Pf1N1B4_5782 0.57 +0.0 221,113 + Pf1N1B4_5782 0.58 +0.0 221,116 - Pf1N1B4_5782 0.58 -3.6 221,244 + Pf1N1B4_5782 0.63 +0.0 221,245 - -3.0 221,684 + Pf1N1B4_5782 0.83 +0.0 221,684 + +0.0 221,684 + Pf1N1B4_5782 0.83 -2.3 221,688 + Pf1N1B4_5782 0.84 +0.0 221,688 + Pf1N1B4_5782 0.84 +0.0 221,688 + Pf1N1B4_5782 0.84 +0.0 221,688 + Pf1N1B4_5782 0.84 -2.9 221,688 + Pf1N1B4_5782 0.84 +0.0 221,688 + Pf1N1B4_5782 0.84 +0.0 221,688 + Pf1N1B4_5782 0.84 -2.5 221,688 + Pf1N1B4_5782 0.84 -2.3 221,689 - Pf1N1B4_5782 0.84 +0.0 222,056 - -1.3 222,493 + Pf1N1B4_5783 0.26 -2.1 222,494 - Pf1N1B4_5783 0.26 +0.1 222,494 - Pf1N1B4_5783 0.26 -1.9 222,649 + Pf1N1B4_5783 0.37 +0.1 222,649 + Pf1N1B4_5783 0.37 +0.1 222,650 - Pf1N1B4_5783 0.38 -1.4 222,650 - Pf1N1B4_5783 0.38 +0.1 222,650 - Pf1N1B4_5783 0.38 -0.2 222,655 + Pf1N1B4_5783 0.38 -1.5 222,776 - Pf1N1B4_5783 0.47 +0.1 222,781 + -1.4
Or see this region's nucleotide sequence