Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5747 and Pf1N1B4_5748 are separated by 30 nucleotides Pf1N1B4_5748 and Pf1N1B4_5749 are separated by 262 nucleotides Pf1N1B4_5749 and Pf1N1B4_5750 are separated by 240 nucleotides Pf1N1B4_5750 and Pf1N1B4_5751 are separated by 78 nucleotides
Pf1N1B4_5747 - Molybdopterin biosynthesis protein MoeA, at 188,759 to 189,538
_5747
Pf1N1B4_5748 - hypothetical protein, at 189,569 to 189,727
_5748
Pf1N1B4_5749 - Molybdenum cofactor biosynthesis protein MoaB, at 189,990 to 190,544
_5749
Pf1N1B4_5750 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 190,785 to 191,432
_5750
Pf1N1B4_5751 - Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR, at 191,511 to 193,040
_5751
Position (kb) 189
190
191 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 189.009 kb on - strand, within Pf1N1B4_5747 at 189.316 kb on - strand, within Pf1N1B4_5747 at 189.378 kb on + strand at 189.378 kb on + strand, within Pf1N1B4_5747 at 189.378 kb on + strand, within Pf1N1B4_5747 at 189.379 kb on - strand, within Pf1N1B4_5747 at 189.593 kb on - strand, within Pf1N1B4_5748 at 190.158 kb on - strand, within Pf1N1B4_5749 at 190.379 kb on - strand, within Pf1N1B4_5749 at 190.415 kb on + strand, within Pf1N1B4_5749 at 190.415 kb on + strand, within Pf1N1B4_5749 at 190.415 kb on + strand, within Pf1N1B4_5749 at 190.416 kb on - strand, within Pf1N1B4_5749 at 190.416 kb on - strand, within Pf1N1B4_5749 at 190.416 kb on - strand, within Pf1N1B4_5749 at 190.624 kb on - strand at 190.640 kb on - strand at 190.773 kb on + strand at 190.813 kb on - strand at 190.813 kb on - strand at 191.057 kb on - strand, within Pf1N1B4_5750 at 191.188 kb on + strand, within Pf1N1B4_5750 at 191.189 kb on - strand, within Pf1N1B4_5750 at 191.399 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 189,009 - Pf1N1B4_5747 0.32 +0.0 189,316 - Pf1N1B4_5747 0.71 -2.5 189,378 + +0.0 189,378 + Pf1N1B4_5747 0.79 -2.2 189,378 + Pf1N1B4_5747 0.79 +0.0 189,379 - Pf1N1B4_5747 0.79 -2.1 189,593 - Pf1N1B4_5748 0.15 -0.9 190,158 - Pf1N1B4_5749 0.30 -2.8 190,379 - Pf1N1B4_5749 0.70 +0.0 190,415 + Pf1N1B4_5749 0.77 +0.0 190,415 + Pf1N1B4_5749 0.77 -3.4 190,415 + Pf1N1B4_5749 0.77 +0.0 190,416 - Pf1N1B4_5749 0.77 -4.2 190,416 - Pf1N1B4_5749 0.77 +0.0 190,416 - Pf1N1B4_5749 0.77 -1.8 190,624 - +0.0 190,640 - -2.6 190,773 + +0.0 190,813 - +0.0 190,813 - +0.0 191,057 - Pf1N1B4_5750 0.42 +0.0 191,188 + Pf1N1B4_5750 0.62 +0.0 191,189 - Pf1N1B4_5750 0.62 -1.7 191,399 - +0.0
Or see this region's nucleotide sequence