Experiment: Nitrate reduction, replicate A t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5744 and Pf1N1B4_5745 overlap by 7 nucleotides Pf1N1B4_5745 and Pf1N1B4_5746 are separated by 10 nucleotides Pf1N1B4_5746 and Pf1N1B4_5747 are separated by 132 nucleotides Pf1N1B4_5747 and Pf1N1B4_5748 are separated by 30 nucleotides
Pf1N1B4_5744 - FIG138517: Putative lipid carrier protein, at 186,266 to 186,736
_5744
Pf1N1B4_5745 - FIG139928: Putative protease, at 186,730 to 187,620
_5745
Pf1N1B4_5746 - FIG139552: Putative protease, at 187,631 to 188,626
_5746
Pf1N1B4_5747 - Molybdopterin biosynthesis protein MoeA, at 188,759 to 189,538
_5747
Pf1N1B4_5748 - hypothetical protein, at 189,569 to 189,727
_5748
Position (kb) 187
188
189 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 186.840 kb on - strand, within Pf1N1B4_5745 at 187.094 kb on + strand, within Pf1N1B4_5745 at 187.263 kb on - strand, within Pf1N1B4_5745 at 187.788 kb on + strand, within Pf1N1B4_5746 at 187.788 kb on + strand at 188.085 kb on + strand, within Pf1N1B4_5746 at 188.086 kb on - strand at 188.123 kb on + strand, within Pf1N1B4_5746 at 188.123 kb on + strand, within Pf1N1B4_5746 at 188.124 kb on - strand, within Pf1N1B4_5746 at 188.124 kb on - strand at 188.180 kb on + strand, within Pf1N1B4_5746 at 188.181 kb on - strand, within Pf1N1B4_5746 at 188.241 kb on + strand, within Pf1N1B4_5746 at 188.242 kb on - strand, within Pf1N1B4_5746 at 188.322 kb on + strand, within Pf1N1B4_5746 at 188.322 kb on + strand, within Pf1N1B4_5746 at 188.322 kb on + strand, within Pf1N1B4_5746 at 188.553 kb on + strand at 188.554 kb on - strand at 188.944 kb on - strand, within Pf1N1B4_5747 at 189.009 kb on - strand, within Pf1N1B4_5747 at 189.316 kb on - strand, within Pf1N1B4_5747 at 189.378 kb on + strand at 189.378 kb on + strand, within Pf1N1B4_5747 at 189.378 kb on + strand, within Pf1N1B4_5747 at 189.379 kb on - strand, within Pf1N1B4_5747 at 189.593 kb on - strand, within Pf1N1B4_5748
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1 remove 186,840 - Pf1N1B4_5745 0.12 -3.3 187,094 + Pf1N1B4_5745 0.41 -2.5 187,263 - Pf1N1B4_5745 0.60 -3.3 187,788 + Pf1N1B4_5746 0.16 -2.9 187,788 + -3.1 188,085 + Pf1N1B4_5746 0.46 +0.0 188,086 - +0.0 188,123 + Pf1N1B4_5746 0.49 -3.1 188,123 + Pf1N1B4_5746 0.49 -3.0 188,124 - Pf1N1B4_5746 0.49 +0.0 188,124 - +0.0 188,180 + Pf1N1B4_5746 0.55 +0.0 188,181 - Pf1N1B4_5746 0.55 +0.0 188,241 + Pf1N1B4_5746 0.61 -3.8 188,242 - Pf1N1B4_5746 0.61 +0.0 188,322 + Pf1N1B4_5746 0.69 -3.6 188,322 + Pf1N1B4_5746 0.69 +0.0 188,322 + Pf1N1B4_5746 0.69 -2.2 188,553 + +0.0 188,554 - +0.0 188,944 - Pf1N1B4_5747 0.24 -1.6 189,009 - Pf1N1B4_5747 0.32 +0.0 189,316 - Pf1N1B4_5747 0.71 -2.5 189,378 + +0.0 189,378 + Pf1N1B4_5747 0.79 -2.2 189,378 + Pf1N1B4_5747 0.79 +0.0 189,379 - Pf1N1B4_5747 0.79 -2.1 189,593 - Pf1N1B4_5748 0.15 -0.9
Or see this region's nucleotide sequence