Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5735

Experiment: Nitrate reduction, replicate A t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5734 and Pf1N1B4_5735 are separated by 1 nucleotidesPf1N1B4_5735 and Pf1N1B4_5736 are separated by 68 nucleotides Pf1N1B4_5734 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 173,287 to 174,825 _5734 Pf1N1B4_5735 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 174,827 to 178,600 _5735 Pf1N1B4_5736 - Nitrate/nitrite transporter, at 178,669 to 180,066 _5736 Position (kb) 174 175 176 177 178 179Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 173.838 kb on - strand, within Pf1N1B4_5734at 174.132 kb on - strand, within Pf1N1B4_5734at 174.224 kb on + strand, within Pf1N1B4_5734at 174.293 kb on + strand, within Pf1N1B4_5734at 174.293 kb on + strandat 174.293 kb on + strand, within Pf1N1B4_5734at 174.294 kb on - strand, within Pf1N1B4_5734at 174.294 kb on - strand, within Pf1N1B4_5734at 174.294 kb on - strand, within Pf1N1B4_5734at 174.525 kb on - strand, within Pf1N1B4_5734at 174.528 kb on - strand, within Pf1N1B4_5734at 174.714 kb on - strandat 174.955 kb on - strandat 174.957 kb on + strandat 174.958 kb on - strandat 174.958 kb on - strandat 174.958 kb on - strandat 175.101 kb on + strandat 175.102 kb on - strandat 175.284 kb on + strand, within Pf1N1B4_5735at 175.285 kb on - strand, within Pf1N1B4_5735at 175.522 kb on - strand, within Pf1N1B4_5735at 175.902 kb on + strandat 176.412 kb on + strand, within Pf1N1B4_5735at 176.412 kb on + strand, within Pf1N1B4_5735at 176.412 kb on + strand, within Pf1N1B4_5735at 176.544 kb on + strand, within Pf1N1B4_5735at 176.544 kb on + strand, within Pf1N1B4_5735at 176.545 kb on - strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.655 kb on + strand, within Pf1N1B4_5735at 176.754 kb on + strand, within Pf1N1B4_5735at 176.859 kb on + strand, within Pf1N1B4_5735at 177.105 kb on + strand, within Pf1N1B4_5735at 177.294 kb on + strand, within Pf1N1B4_5735at 177.615 kb on + strand, within Pf1N1B4_5735at 177.615 kb on + strand, within Pf1N1B4_5735at 177.627 kb on + strand, within Pf1N1B4_5735at 177.750 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strandat 177.858 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strand, within Pf1N1B4_5735at 177.858 kb on + strand, within Pf1N1B4_5735at 177.859 kb on - strand, within Pf1N1B4_5735at 177.871 kb on - strand, within Pf1N1B4_5735at 177.951 kb on + strand, within Pf1N1B4_5735at 177.952 kb on - strand, within Pf1N1B4_5735at 177.952 kb on - strand, within Pf1N1B4_5735at 177.988 kb on - strand, within Pf1N1B4_5735at 178.063 kb on - strand, within Pf1N1B4_5735at 178.075 kb on + strand, within Pf1N1B4_5735at 178.076 kb on - strand, within Pf1N1B4_5735at 178.077 kb on + strand, within Pf1N1B4_5735at 178.077 kb on + strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.078 kb on - strand, within Pf1N1B4_5735at 178.156 kb on + strand, within Pf1N1B4_5735at 178.159 kb on - strand, within Pf1N1B4_5735at 178.159 kb on - strand, within Pf1N1B4_5735at 178.290 kb on + strandat 178.291 kb on - strandat 178.296 kb on + strandat 178.296 kb on + strandat 178.414 kb on - strandat 178.589 kb on + strandat 178.590 kb on - strandat 178.733 kb on + strandat 178.751 kb on + strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 178.752 kb on - strandat 179.406 kb on + strand, within Pf1N1B4_5736at 179.406 kb on + strand, within Pf1N1B4_5736at 179.407 kb on - strand, within Pf1N1B4_5736at 179.407 kb on - strand, within Pf1N1B4_5736at 179.407 kb on - strandat 179.505 kb on + strand, within Pf1N1B4_5736

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A t1
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173,838 - Pf1N1B4_5734 0.36 +0.0
174,132 - Pf1N1B4_5734 0.55 +0.0
174,224 + Pf1N1B4_5734 0.61 -3.7
174,293 + Pf1N1B4_5734 0.65 +0.0
174,293 + -1.0
174,293 + Pf1N1B4_5734 0.65 -1.8
174,294 - Pf1N1B4_5734 0.65 +0.0
174,294 - Pf1N1B4_5734 0.65 +0.0
174,294 - Pf1N1B4_5734 0.65 +0.0
174,525 - Pf1N1B4_5734 0.80 -2.6
174,528 - Pf1N1B4_5734 0.81 -3.4
174,714 - -4.2
174,955 - +0.0
174,957 + -2.8
174,958 - -2.5
174,958 - -2.9
174,958 - +0.0
175,101 + -4.5
175,102 - +0.0
175,284 + Pf1N1B4_5735 0.12 -2.8
175,285 - Pf1N1B4_5735 0.12 -4.2
175,522 - Pf1N1B4_5735 0.18 +0.0
175,902 + +0.0
176,412 + Pf1N1B4_5735 0.42 -4.9
176,412 + Pf1N1B4_5735 0.42 -3.3
176,412 + Pf1N1B4_5735 0.42 +0.0
176,544 + Pf1N1B4_5735 0.45 -2.9
176,544 + Pf1N1B4_5735 0.45 -0.9
176,545 - Pf1N1B4_5735 0.46 -4.8
176,655 + Pf1N1B4_5735 0.48 -2.5
176,655 + Pf1N1B4_5735 0.48 -4.0
176,655 + Pf1N1B4_5735 0.48 -3.9
176,655 + Pf1N1B4_5735 0.48 +0.0
176,754 + Pf1N1B4_5735 0.51 +0.0
176,859 + Pf1N1B4_5735 0.54 -3.8
177,105 + Pf1N1B4_5735 0.60 -2.7
177,294 + Pf1N1B4_5735 0.65 -3.0
177,615 + Pf1N1B4_5735 0.74 -2.5
177,615 + Pf1N1B4_5735 0.74 -1.9
177,627 + Pf1N1B4_5735 0.74 -3.0
177,750 + Pf1N1B4_5735 0.77 -3.3
177,858 + -4.7
177,858 + Pf1N1B4_5735 0.80 +0.0
177,858 + Pf1N1B4_5735 0.80 +0.0
177,858 + Pf1N1B4_5735 0.80 +0.0
177,859 - Pf1N1B4_5735 0.80 -4.3
177,871 - Pf1N1B4_5735 0.81 +0.0
177,951 + Pf1N1B4_5735 0.83 +0.0
177,952 - Pf1N1B4_5735 0.83 -2.9
177,952 - Pf1N1B4_5735 0.83 -3.4
177,988 - Pf1N1B4_5735 0.84 -3.6
178,063 - Pf1N1B4_5735 0.86 -3.4
178,075 + Pf1N1B4_5735 0.86 +0.0
178,076 - Pf1N1B4_5735 0.86 +0.0
178,077 + Pf1N1B4_5735 0.86 -3.0
178,077 + Pf1N1B4_5735 0.86 +0.0
178,078 - Pf1N1B4_5735 0.86 +0.0
178,078 - Pf1N1B4_5735 0.86 +0.0
178,078 - Pf1N1B4_5735 0.86 -4.6
178,156 + Pf1N1B4_5735 0.88 -3.6
178,159 - Pf1N1B4_5735 0.88 +0.0
178,159 - Pf1N1B4_5735 0.88 -2.8
178,290 + -2.3
178,291 - -4.0
178,296 + -3.7
178,296 + -3.0
178,414 - +0.0
178,589 + +0.0
178,590 - -4.1
178,733 + +0.0
178,751 + +0.0
178,752 - +0.0
178,752 - +0.0
178,752 - -2.4
178,752 - -2.5
178,752 - -2.2
178,752 - -3.7
178,752 - +0.0
178,752 - +0.0
179,406 + Pf1N1B4_5736 0.53 +0.0
179,406 + Pf1N1B4_5736 0.53 +0.0
179,407 - Pf1N1B4_5736 0.53 +0.0
179,407 - Pf1N1B4_5736 0.53 -3.1
179,407 - +0.0
179,505 + Pf1N1B4_5736 0.60 -2.9

Or see this region's nucleotide sequence